Designer recombinases for efficient and safe genome surgery

Informazioni relative al progetto

GENSURGE

ID dell’accordo di sovvenzione: 742133

DOI 10.3030/742133

Progetto chiuso

Data della firma CE 27 Giugno 2017

Data di avvio 1 Gennaio 2018

Data di completamento 30 Giugno 2024

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale € 2 380 425,00

Contributo UE € 2 380 425,00

Coordinato da

TECHNISCHE UNIVERSITAET DRESDEN
Germany

Pubblicazioni

Peer reviewed articles (7)

Conformational dynamics promotes disordered regions from function-dispensable to essential in evolved site-specific DNA recombinases

Autori: Carla Guillén-Pingarrón, Pedro M Guillem-Gloria, Anjali Soni, Gloria Ruiz-Gómez, Martina Augsburg, Frank Buchholz, Massimiliano Anselmi, M Teresa Pisabarro
Pubblicato in: Computational and Structural Biotechnology Journal, Numero Volume 20, 2022, Pagina/e 989-1001, ISSN 2001-0370
Editore: Research Network of Computational and Structural
DOI: 10.1016/j.csbj.2022.01.010

Pairing of single mutations yields obligate Cre-type site-specific recombinases

Autori: Jenna Hoersten, Gloria Ruiz-Gómez, Felix Lansing, Teresa Rojo-Romanos, Lukas Theo Schmitt, Jan Sonntag, M Teresa Pisabarro, Frank Buchholz
Pubblicato in: Nucleic Acids Research, Numero Volume 50 Numero 2, 2022, Pagina/e 1174–1186, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkab1240

A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination

Autori: Madina Karimova, Oliver Baker, Aylin Camgoz, Ronald Naumann, Frank Buchholz, Konstantinos Anastassiadis
Pubblicato in: Scientific Reports, Numero 8/1, 2018, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-32802-7

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

Autori: Duran Sürün, Aksana Schneider, Jovan Mircetic, Katrin Neumann, Felix Lansing, Maciej Paszkowski-Rogacz, Vanessa Hänchen, Min Ae Lee-Kirsch, Frank Buchholz
Pubblicato in: Genes, Numero 11/5, 2020, Pagina/e 511, ISSN 2073-4425
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes11050511

A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus

Autori: Felix Lansing, Maciej Paszkowski-Rogacz, Lukas Theo Schmitt, Paul Martin Schneider, Teresa Rojo Romanos, Jan Sonntag, Frank Buchholz
Pubblicato in: Nucleic Acids Research, Numero 48/1, 2019, Pagina/e 472-485, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkz1078

Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases

Autori: Anjali Soni, Martina Augsburg, Frank Buchholz, M. Teresa Pisabarro
Pubblicato in: Scientific Reports, Numero 10/1, 2020, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-020-70867-5

Correction of a Factor VIII genomic inversion with designer-recombinases

Autori: Felix Lansing, Liliya Mukhametzyanova, Teresa Rojo-Romanos, Kentaro Iwasawa, Masaki Kimura, Maciej Paszkowski-Rogacz, Janet Karpinski, Tobias Grass, Jan Sonntag, Paul Martin Schneider, Ceren Günes, Jenna Hoersten, Lukas Theo Schmitt, Natalia Rodriguez-Muela, Ralf Knöfler, Takanori Takebe & Frank Buchholz
Pubblicato in: Nature Communications, Numero 422 (2022), 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-28080-7