Yeast Biotechnology Doctoral Training Programme

Leistungen

UCC will manage the advertisement of positions on EURAXESS. Each beneficiary will also advertise through their own channels and contacts.

At least 6 joint research publications in high impact journals

Joint research publications in high impact journals will be a measure of the success of the project and the consortium. The aim is to publish 6 such joint publications during the lifetime of the project.

An appraisal of the potential application of novel, flavour-producing yeast strains for production of fermented beverages.

YEASTDOC will apply advanced genetic tools to understand how different genes and alleles interact with each other and the environment to produce flavours and aromas in fermented and distilled beverages The potential for new hybrids and process conditions to be used in industry will be evaluated in conjunction with industrial partners Where appropriate plans for advancing the research to a higher TRL will be developed

A report describing a set of genes or alleles that individually or in combination enhance the industrial potential of yeast strains.

Genes and alleles that contribute to the improved aroma and flavour of fermented beverages will be identified in this project Strains that cope with both substrate and productinduced cellular stress and that have the ability to grow on inexpensive substrates and efficiently secrete the product will also be identified A report will be compiled describing the genes that contribute to these beneficial traits

A communication and engagement plan

A communication and engagement plan describing each activity, personnel involved, timeline for completion, and Key Performance Indicators (KPIs) for assessing delivery and impact, will be drawn up. This plan will be reviewed and updated annually.

An appraisal of whether the engineering strategies identified for improving industrial yeasts are effective and suitable for the improvement of diverse yeast cell factories

Engineering strategies such as genome engineering ALE reverse engineering will be utilized in the project This report will evaluate the effectiveness of such strategies

A report detailing the effectiveness of public communication and engagement strategy

A detailed public engagement plan will have been prepared at the beginning of the project This report will evaluate how successful this strategy was at engaging with the target audiences and at influencing perceptions both in the ESRs and the target audiences

A report on the effectiveness of the Industry engagement activities

The first route to exploitation of project outcomes naturally involves the Partner Organisations from YEASTDOC but there will also be broadly applicable outcomes that can benefit industry more widely Hence the dissemination plan will have specific components to promote engagement with additional potential industry endusers This includes interaction with industry platforms and societies This report will examine how effective these engagement strategies have been

A review of the application of advanced genetics for strain selection in beverage yeasts

A new methodology developed by ULEIC that involves mating diploids to overcome hybrid sterility followed by multigenerational crosses will be employed in YEASTDOC This leads to a huge increase in gene assortment relative to simple crosses and a large increase in the diversity of the hybrid population These populations will then be used in a screening and genetics programme to identify hybrids with novel flavouraroma characteristics and the genes responsible for this The project will also develop QTL approaches to map complex traits applying these methods to identify the basis of several important fermentation traits This report will evaluate the effectiveness of these methodologies

A public access website

A public website detailing the consortium and the research & training activities of YEASTDOC will be created.

Veröffentlichungen

Interdependence between lignocellulosic biomasses, enzymatic hydrolysis and yeast cell factories in biorefineries.

Autoren: Bertacchi, S., Jayaprakash, P., Morrissey, J.P., Branduardi, P.
Veröffentlicht in: Microbial Biotechnology, Ausgabe 17517915, 2021, ISSN 1751-7915
Herausgeber: Wiley
DOI: 10.1111/1751-7915.13886

The Dicarboxylate Transporters from the AceTr Family and Dct-02 Oppositely Affect Succinic Acid

Autoren: Rendulić T, Mendonça Bahia F, Soares-Silva I, Nevoigt E, Casal M.
Veröffentlicht in: Journal of Fungi, Ausgabe 2309608X, 2022, ISSN 2309-608X
Herausgeber: MDPI
DOI: 10.3390/jof8080822

The evolution and role of the periplasmic asparaginase Asp3 in yeast. . 22, 1–11. https://doi.org/10.1093/femsyr/foac044

Autoren: Coral-Medina, A., Fenton, D.A., Varela, J., Baranov, P. V, Camarasa, C., Morrissey, J.P.
Veröffentlicht in: FEMS Yeast Research, Ausgabe 15671356, 2022, Seite(n) 1 - 11, ISSN 1567-1356
Herausgeber: Blackwell
DOI: 10.1093/femsyr/foac044

Carbon dioxide fixation via production of succinic acid from glycerol in engineered Saccharomyces cerevisiae

Autoren: Zahabiya Malubhoy; Frederico Mendonça Bahia; Sophie Claire de Valk; Erik de Hulster; Toni Rendulić; Juan Paulo Ragas Ortiz; Joeline Xiberras; Mathias Klein; Robert Mans; Elke Nevoigt
Veröffentlicht in: Microbial Cell Factories, Ausgabe 14752859, 2022, ISSN 1475-2859
Herausgeber: BioMed Central
DOI: 10.1186/s12934-022-01817-1

Yeast Plasma Membrane Fungal Oligopeptide Transporters Display Distinct Substrate Preferences despite Their High Sequence Identity.

Autoren: Becerra-Rodríguez, C., Taghouti, G., Portier, P., Dequin, S., Casal, M., Paiva, S., et al.
Veröffentlicht in: Journal of Fungi, Ausgabe 2309608X, 2021, ISSN 2309-608X
Herausgeber: MDPI
DOI: 10.3390/jof7110963

Identification of novel pentose transporters in Kluyveromyces marxianus using a new screening platform.

Autoren: Lorena Donzella; Javier A. Varela; Maria João Sousa; John P. Morrissey
Veröffentlicht in: FEMS Yeast Research, Ausgabe 15671356, 2021, ISSN 1567-1356
Herausgeber: Blackwell
DOI: 10.1093/femsyr/foab026

Hexose transport in Torulaspora delbrueckii: identification of Igt1, a new dual-affinity transporter

Autoren: Andreia Pacheco, Lorena Donzella, Maria Jose Hernandez-Lopez, Maria Judite Almeida, Jose Antonio Prieto, Francisca Randez-Gil, John P Morrissey, Maria João Sousa
Veröffentlicht in: FEMS Yeast Research, Ausgabe 20/1, 2020, ISSN 1567-1356
Herausgeber: Blackwell
DOI: 10.1093/femsyr/foaa004

Diversity of Oligopeptide Transport in Yeast and Its Impact on Adaptation to Winemaking Conditions

Autoren: Carmen Becerra-Rodríguez, Souhir Marsit, Virginie Galeote
Veröffentlicht in: Frontiers in Genetics, Ausgabe 11, 2020, ISSN 1664-8021
Herausgeber: Frontiers Media
DOI: 10.3389/fgene.2020.00602

Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications

Autoren: I Soares-Silva, D Ribas, M Sousa-Silva, J Azevedo-Silva, T Rendulić, M Casal
Veröffentlicht in: FEMS Microbiology Letters, 2020, ISSN 0378-1097
Herausgeber: Blackwell
DOI: 10.1093/femsle/fnaa118

Beyond Saccharomyces pastorianus for modern lager brews: Exploring non-cerevisiae Saccharomyces hybrids with heterotic maltotriose consumption and novel aroma profile.

Autoren: Gyurchev, N.Y., Coral-Medina, Á., Weening, S.M., Almayouf, S., Kuijpers, N.G.A., Nevoigt, E., Louis, E.J.
Veröffentlicht in: Frontiers Microbiology, Ausgabe 1664302X, 2022, ISSN 1664-302X
Herausgeber: Frontiers Media
DOI: 10.3389/fmicb.2022.1025132

Impact of ergosterol content on acetic and lactic acids toxicity to Saccharomyces cerevisiae

Autoren: Luís Ferraz, Karola Vorauer-Uhl, Michael Sauer, Maria J. Sousa, Paola Branduardi
Veröffentlicht in: Yeast, 2022, ISSN 1097-0061
Herausgeber: John Wiley & Sons Ltd
DOI: 10.1002/yea.3828

The growth and metabolome of Saccharomyces uvarum in wine fermentations is strongly influenced by the route of nitrogen assimilation.

Autoren: Coral-Medina, A., Morrissey, J.P., Camarasa, C.
Veröffentlicht in: J. Ind. Microbiol. Biotechnol., Ausgabe 14765535, 2022, ISSN 1476-5535
Herausgeber: Oxford University Press
DOI: 10.1093/jimb/kuac025

New insights into the acetate uptake transporter (AceTr) family: Unveiling amino acid residues critical for specificity and activity.

Autoren: Rendulić, T. Alves, J., Azevedo-Silva,J. Soares-Silva,I., Casal,M.
Veröffentlicht in: Computational and Structural Biotechnology Journal, Ausgabe 20010370, 2021, ISSN 2001-0370
Herausgeber: Elsevier
DOI: 10.1016/j.csbj.2021.08.002

Insights on life cycle and cell identity regulatory circuits for unlocking genetic improvement in Zygosaccharomyces and Kluyveromyces yeasts.

Autoren: Lisa Solieri; Stefano Cassanelli; Franziska Huff; Franziska Huff; Liliane Barroso; Liliane Barroso; Paola Branduardi; Edward J. Louis; John P. Morrissey
Veröffentlicht in: FEMS Yeast Research, Ausgabe 15671356, 2021, ISSN 1567-1356
Herausgeber: Blackwell
DOI: 10.1093/femsyr/foab058

The plasma Membrane at the cornerstone between flexibility and adaptability: implications for Saccharomyces cerevisiae as a cell factory.

Autoren: Ferraz, L., Sauer, M., Sousa, M.J., Branduardi, P.
Veröffentlicht in: Frontiers Microbiology, Ausgabe 1664302x, 2021, ISSN 1664-302x
Herausgeber: Frontiers Media
DOI: 10.3389/fmicb.2021.715891

Regulated use of alternative Transcription Start Sites controls the production of cytosolic or mitochondrial forms of branched-chain aminotransferase in Kluyveromyces marxianus

Autoren: Angela Coral-Medina, Darren A. Fenton, Javier Varela, Carole Camarasa, Pavel V. Baranov, John P. Morrissey
Veröffentlicht in: bioRxiv, Ausgabe 26928205, 2022, ISSN 2692-8205
Herausgeber: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.04.27.489738

Leistungen

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