TArgeting Platelet Adhesion receptors in thrombosiS

Risultati finali

Progress report

Progress report of the project.

Publication of e-Newsletters

D73 Publication of eNewsletters M9 21 33 42

Setup of TAPAS website (updates every 6 months)

D6.2 Setup of TAPAS website setup (M3) with updates every 6 months

Execution of dissemination and Outreach activities for ITN

D72 Execution of dissemination and outreach activities for ITN M17152739

Organisation of first European Summer School

D5.4: Organisation of European Summer School (M11)

Organsation of Training Workshops and Conferences

Organisation of Training Workshops and Conferences M817232939

Recruitment of 15 ESRs (with statistics of applications)

D6.3 Recruited 15 ESRs (with statistics of applications) (M9)

Dissemination and Outreach activity plan completed

D7.1 Dissemination and Outreach activity plan completed (M10)

15 PhD theses with awarded EJD degrees

D74 15 PhD theses with awarded EJD degrees M48

Organisation of second European Summer School

Organisation of second European Summer School M31

Organisation of Training Workshops and Conferences

D5.3: Organisation of Training Workshops and Conferences (M8,17,23,29,39

Pubblicazioni

Multiparameter microfluidics assay of thrombus formation reveals increased sensitivity to contraction and antiplatelet agents at physiological temperature

Autori: Linda Herfs, Frauke Swieringa, Natalie Jooss, Mike Kozlowski, Floor C.J. Heubel-Moenen, René van Oerle, Patric Machiels, Yvonne Henskens, Johan W.M. Heemskerk
Pubblicato in: Thrombosis Research, Numero 203, 2021, Pagina/e 46-56, ISSN 0049-3848
Editore: Pergamon Press
DOI: 10.1016/j.thromres.2021.04.014

Targeted Phosphoinositides Analysis Using High-Performance Ion Chromatography-Coupled Selected Reaction Monitoring Mass Spectrometry

Autori: Hilaire Yam Fung Cheung, Cristina Coman, Philipp Westhoff, Mailin Manke, Albert Sickmann, Oliver Borst, Meinrad Gawaz, Steve P. Watson, Johan W. M. Heemskerk, Robert Ahrends
Pubblicato in: Journal of Proteome Research, Numero 20/6, 2021, Pagina/e 3114-3123, ISSN 1535-3893
Editore: American Chemical Society
DOI: 10.1021/acs.jproteome.1c00017

Effects of platelet agonists and priming on 1 the formation of platelet populations

Autori: Veninga A, Baaten C, De Simone I, Tullemans B, Kuijpers M, Heemskerk J, van der Meijden P
Pubblicato in: Thrombosis and Haemostasis, 2021, ISSN 0340-6245
Editore: Schattauer
DOI: 10.1055/s-0041-1735972

Rac Inhibition Causes Impaired GPVI Signalling in Human Platelets through GPVI Shedding and Reduction in PLCγ2 Phosphorylation

Autori: Neagoe, R.A.I.; Gardiner, E.E.; Stegner, D.; Nieswandt, B.; Watson, S.P.; Poulter, N.S.
Pubblicato in: International Journal of Molecular Sciences, Numero 23(7):3746, 2022, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23073746

Analysis of platelets from a diet-induced obesity rat model: elucidating platelet dysfunction in obesity

Autori: María N. Barrachina, Luis A. Morán, Irene Izquierdo, Felipe F. Casanueva, María Pardo, Ángel García
Pubblicato in: Scientific Reports, Numero 10/1, 2020, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-020-70162-3

Platelet calcium signaling by G-protein coupled and ITAM-linked receptors regulating anoctamin-6 and procoagulant activity

Autori: Delia I. Fernández, Marijke J. E. Kuijpers, Johan W. M. Heemskerk
Pubblicato in: Platelets, Numero 32/7, 2021, Pagina/e 863-871, ISSN 0953-7104
Editore: Taylor & Francis
DOI: 10.1080/09537104.2020.1859103

Targeting platelet inhibition receptors for novel therapies: PECAM-1 and G6b-B

Autori: Soriano Jerez, E.M., J.M. Gibbins, and C.E. Hughes
Pubblicato in: Platelets, 2021, ISSN 0953-7104
Editore: Taylor & Francis
DOI: 10.1080/09537104.2021.1882668

Phosphoproteomic Analysis of Platelets in Severe Obesity Uncovers Platelet Reactivity and Signaling Pathways Alterations

Autori: María N. Barrachina, Lidia Hermida-Nogueira, Luis A. Moran, Vanessa Casas, Sarah M. Hicks, Aurelio M. Sueiro, Ying Di, Robert K. Andrews, Steve P. Watson, Elizabeth E. Gardiner, Joaquin Abian, Montserrat Carrascal, María Pardo, Ángel García
Pubblicato in: Arteriosclerosis, Thrombosis, and Vascular Biology, 2020, ISSN 1079-5642
Editore: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/atvbaha.120.314485

Structure-function relationship of the platelet glycoprotein VI (GPVI) receptor: does it matter if it is a dimer or monomer?

Autori: Joanne C. Clark, Foteini-Nafsika Damaskinaki, Yam Fung Hilaire Cheung, Alexandre Slater, Steve P. Watson
Pubblicato in: Platelets, 2021, Pagina/e 1-8, ISSN 0953-7104
Editore: Taylor & Francis
DOI: 10.1080/09537104.2021.1887469

Platelet-primed interactions of coagulation and anticoagulation pathways in flow-dependent thrombus formation

Autori: Sanne L. N. Brouns, Johanna P. van Geffen, Elena Campello, Frauke Swieringa, Luca Spiezia, René van Oerle, Isabella Provenzale, Remco Verdoold, Richard W. Farndale, Kenneth J. Clemetson, Henri M. H. Spronk, Paola E. J. van der Meijden, Rachel Cavill, Marijke J. E. Kuijpers, Elisabetta Castoldi, Paolo Simioni, Johan W. M. Heemskerk
Pubblicato in: Scientific Reports, Numero 10/1, 2020, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-020-68438-9

The PI3Kδ Inhibitor Idelalisib Diminishes Platelet Function and Shows Antithrombotic Potential

Autori: María N. Barrachina, Irene Izquierdo, Lidia Hermida-Nogueira, Luis A. Morán, Amparo Pérez, Ana B. Arroyo, Nuria García-Barberá, Rocío González-Conejero, Sara Troitiño, Johannes A. Eble, José Rivera, Constantino Martínez, María I. Loza, Eduardo Domínguez, Ángel García
Pubblicato in: International Journal of Molecular Sciences, Numero 22/7, 2021, Pagina/e 3304, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22073304

Overcoming challenges in developing small molecule inhibitors for GPVI and CLEC-2

Autori: Foteini-Nafsika Damaskinaki, Luis A. Moran, Angel Garcia, Barrie Kellam, Steve P. Watson
Pubblicato in: Platelets, 2021, Pagina/e 1-9, ISSN 0953-7104
Editore: Taylor & Francis
DOI: 10.1080/09537104.2020.1863939

Role of Platelet Glycoprotein VI and Tyrosine Kinase Syk in Thrombus Formation on Collagen-Like Surfaces

Autori: Natalie J. Jooss, Ilaria De Simone, Isabella Provenzale, Delia I. Fernández, Sanne L.N. Brouns, Richard W. Farndale, Yvonne M.C. Henskens, Marijke J.E. Kuijpers, Hugo ten Cate, Paola E.J. van der Meijden, Rachel Cavill, Johan W.M. Heemskerk
Pubblicato in: International Journal of Molecular Sciences, Numero 20/11, 2019, Pagina/e 2788, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20112788

Interspecies differences in protein expression do not impact the spatiotemporal regulation of GPVI mediated activation

Autori: Joanne L. Dunster, Amanda J. Unsworth, Alexander P. Bye, Elizabeth J Haining, Marcin A. Sowa, Ying Di, Tanya Sage, Chiara Pallini, Jeremy A. Pike, Alexander.T. Hardy, Bernhard Nieswandt, Ángel García, Steve P. Watson, Natalie S. Poulter, Jonathan M. Gibbins, Alice Y. Pollitt
Pubblicato in: Journal of Thrombosis and Haemostasis, 2019, ISSN 1538-7933
Editore: Blackwell Publishing Inc.
DOI: 10.1111/jth.14673

Whole Blood Based Multiparameter Assessment of Thrombus Formation in Standard Microfluidic Devices to Proxy In Vivo Haemostasis and Thrombosis

Autori: Isabella Provenzale, Sanne L. N. Brouns, Paola E. J. van der Meijden, Frauke Swieringa, Johan W. M. Heemskerk
Pubblicato in: Micromachines, Numero 10/11, 2019, Pagina/e 787, ISSN 2072-666X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi10110787

A Comprehensive Tyrosine Phosphoproteomic Analysis Reveals Novel Components of the Platelet CLEC-2 Signaling Cascade

Autori: Irene Izquierdo, María N. Barrachina, Lidia Hermida-Nogueira, Vanessa Casas, Luis A. Morán, Serena Lacerenza, Roberto Pinto-Llorente, Johannes A. Eble, Vivian de los Ríos, Eduardo Domínguez, María I. Loza, José Ignacio Casal, Montserrat Carrascal, Joaquín Abián, Angel García
Pubblicato in: Thrombosis and Haemostasis, Numero 120/02, 2020, Pagina/e 262-276, ISSN 0340-6245
Editore: Schattauer
DOI: 10.1055/s-0039-3400295

Localised endothelial‐based control of platelet aggregation and coagulation under flow: a proof‐of‐principle vessel‐on‐a‐chip study

Autori: Sanne L.N. Brouns, Isabella Provenzale, Johanna P. van Geffen, Paola E. van der Meijden, Johan W.M. Heemskerk
Pubblicato in: Journal of Thrombosis and Haemostasis, 2019, ISSN 1538-7933
Editore: Blackwell Publishing Inc.
DOI: 10.1111/jth.14719

Thrombo-Inflammation in Cardiovascular Disease: An Expert Consensus Document from the Third Maastricht Consensus Conference on Thrombosis

Autori: Elisa d’Alessandro, Christian Becker, Wolfgang Bergmeier, Christoph Bode, Joshua H. Bourne, Helena Brown, Harry R. Buller, Arina J. ten Cate-Hoek, Vincent ten Cate, Yvonne J. M. van Cauteren, Yam F. H. Cheung, Audrey Cleuren, Danielle Coenen, Harry J. G. M. Crijns, Ilaria de Simone, Sophie C. Dolleman, Christine Espinola Klein, Delia I. Fernandez, Lianne Granneman, Arnoud van t Hof, Peter Henke,
Pubblicato in: Thrombosis and Haemostasis, Numero 120/04, 2020, Pagina/e 538-564, ISSN 0340-6245
Editore: Schattauer
DOI: 10.1055/s-0040-1708035

Temporal Roles of Platelet and Coagulation Pathways in Collagen- and Tissue Factor-Induced Thrombus Formation

Autori: Navarro S, Stegner D, Nieswandt B, Heemskerk JWM, Kuijpers MJE
Pubblicato in: International Journal of Molecular Sciences, 2022, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23010358

Katacine is a new ligand of CLEC-2 that acts as a platelet agonist

Autori: Morán LA, Di Y, Sowa MA, Hermida-Nogueira L, Barrachina MN, Martin E, Mize TH, Clark JC, Eble JA, Moreira D, Pollitt AY, Loza MI, Domínguez E, Watson SP, Garcia A
Pubblicato in: Thrombosis and Haemostasis, 2022, ISSN 0340-6245
Editore: Schattauer
DOI: 10.1055/a-1772-1069

Evidence that GPVI is Expressed as a Mixture of Monomers and Dimers, and that the D2 Domain is not Essential for GPVI Activation

Autori: Joanne C. Clark, Raluca A. I. Neagoe, Malou Zuidscherwoude, Deirdre M. Kavanagh, Alexandre Slater, Eleyna M. Martin, Mark Soave, David Stegner, Bernhard Nieswandt, Natalie S. Poulter, Johan Hummert, Dirk-Peter Herten, Michael G. Tomlinson, Stephen J. Hill, Steve P. Watson
Pubblicato in: Thrombosis and Haemostasis, 2021, ISSN 0340-6245
Editore: Schattauer
DOI: 10.1055/a-1401-5014

Tyrosine Kinase Inhibitor Sunitinib Delays Platelet-Induced Coagulation: Additive Effects of Aspirin

Autori: Delia I. Fernández, Alicia Veninga, Bibian M. E. Tullemans, Constance C. F. M. J. Baaten, Linsey J. F. Peters, Maureen J. B. Aarts, Johannes A. Eble, Elena Campello, Luca Spiezia, Paolo Simioni, Emiel P. C. van der Vorst, Paola E. J. van der Meijden, Johan W. M. Heemskerk, Marijke J. E. Kuijpers
Pubblicato in: Thrombosis and Haemostasis, 2021, ISSN 0340-6245
Editore: Schattauer
DOI: 10.1055/s-0041-1730312

A large-scale histological investigation gives insight into the structure of ischemic stroke thrombi

Autori: Natalie J. Jooss, Natalie S. Poulter
Pubblicato in: Platelets, 2021, Pagina/e 1-4, ISSN 0953-7104
Editore: Taylor & Francis
DOI: 10.1080/09537104.2020.1869713

Clonal hematopoietic mutations linked to platelet traits and the risk of thrombosis or bleeding

Autori: Alicia Veninga, Ilaria De Simone, Johan W.M. Heemskerk, Hugo ten Cate, Paola E.J. van der Meijden
Pubblicato in: Haematologica, Numero 105/8, 2020, Pagina/e 2020-2031, ISSN 0390-6078
Editore: Ferrata Storti Foundation
DOI: 10.3324/haematol.2019.235994

Assessment of a complete and classified platelet proteome from genome-wide transcripts of human platelets and megakaryocytes covering platelet functions

Autori: Jingnan Huang, Frauke Swieringa, Fiorella A. Solari, Isabella Provenzale, Luigi Grassi, Ilaria De Simone, Constance C. F. M. J. Baaten, Rachel Cavill, Albert Sickmann, Mattia Frontini, Johan W. M. Heemskerk
Pubblicato in: Scientific Reports, Numero 11/1, 2021, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-021-91661-x

Nonredundant Roles of Platelet Glycoprotein VI and Integrin αIIbβ3 in Fibrin-Mediated Microthrombus Formation

Autori: Gina Perrella, Jingnan Huang, Isabella Provenzale, Frauke Swieringa, Floor C.J.I. Heubel-Moenen, Richard W. Farndale, Mark Roest, Paola E.J. van der Meijden, Mark Thomas, Robert A.S. Ariëns, Martine Jandrot-Perrus, Steve P. Watson, Johan W.M. Heemskerk
Pubblicato in: Arteriosclerosis, Thrombosis, and Vascular Biology, Numero 41/2, 2021, ISSN 1079-5642
Editore: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/atvbaha.120.314641

Ultra-high-throughput Ca2+ assay in platelets to distinguish ITAM-linked and G-protein-coupled receptor activation

Autori: Fernández, D. I., Provenzale, I., Cheung, H. Y. F., van Groningen, J., Tullemans, B. M. E., Veninga, A., Dunster, J. L., Honarnejad, S., van den Hurk, H., Kuijpers, M. J. E., & Heemskerk, J. W. M.
Pubblicato in: iScience, 2021, ISSN 2589-0042
Editore: Elsevier
DOI: 10.1016/j.isci.2021.103718

Structural characterization of a novel GPVI-nanobody complex reveals a biologically active domain-swapped GPVI dimer

Autori: Alexandre Slater, Ying Di, Joanne C. Clark, Natalie J. Jooss, Eleyna M. Martin, Fawaz Alenazy, Mark R. Thomas, Robert A. S. Ariëns, Andrew B. Herr, Natalie S. Poulter, Jonas Emsley, Steve P. Watson
Pubblicato in: Blood, Numero 137/24, 2021, Pagina/e 3443-3453, ISSN 0006-4971
Editore: American Society of Hematology
DOI: 10.1182/blood.2020009440

Multiparameter Evaluation of the Platelet-Inhibitory Effects of Tyrosine Kinase Inhibitors Used for Cancer Treatment

Autori: Tullemans BME, Veninga A, Fernandez DI, Aarts MJB, Eble JA, van der Meijden PEJ, Heemskerk JWM, Kuijpers MJE
Pubblicato in: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222011199

Antibody-mediated depletion of human CLEC-2 in a novel humanised mouse model

Autori: Helena C Brown, Sarah Beck, Stefano Navarro, Ying Di, Eva M Soriano Jerez, Jana Kaczmarzyk, Steven G. Thomas, Valbona Mirakaj, Steve P Watson, Bernhard Nieswandt, David Stegner
Pubblicato in: bioRxiv, 2021
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.10.03.462933

Risultati finali

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