The role of cell-to-cell variability in pneumococcal virulence and antibiotic resistance

Project Information

PneumoCaTChER

Grant agreement ID: 771534

DOI 10.3030/771534

Project closed

EC signature date 13 April 2018

Start date 1 November 2018

End date 31 October 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 999 735,00

EU contribution € 1 999 735,00

Coordinated by

UNIVERSITE DE LAUSANNE
Switzerland

Publications

Peer reviewed articles (17)

Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq

Author(s): Liselot Dewachter, Julien Dénéréaz, Xue Liu, Vincent de Bakker, Charlotte Costa, Mara Baldry, Jean-Claude Sirard, Jan-Willem Veening
Published in: eLife, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.75607

Harnessing CRISPR-Cas9 for Genome Editing in Streptococcus pneumoniae D39V

Author(s): Dimitra Synefiaridou, Jan-Willem Veening
Published in: Applied and Environmental Microbiology, Issue 87/6, 2021, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.02762-20

CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication by modulating the activity of DnaA

Author(s): Clement Gallay, Stefano Sanselicio, Mary E. Anderson, Young Min Soh, Xue Liu, Gro A. Stamsås, Simone Pelliciari, Renske van Raaphorst, Julien Dénéréaz, Morten Kjos, Heath Murray, Stephan Gruber, Alan D. Grossman, Jan-Willem Veening
Published in: Nature Microbiology, Issue 6/9, 2021, Page(s) 1175-1187, ISSN 2058-5276
Publisher: NPG
DOI: 10.1038/s41564-021-00949-1

Switching off: The phenotypic transition to the uninduced state of the lactose uptake pathway

Author(s): Prasanna M Bhogale, Robin A Sorg, Jan-Willem Veening, Johannes Berg
Published in: Biophys J ., 2022, ISSN 0006-3495
Publisher: Biophysical Society
DOI: 10.1016/j.bpj.2021.12.027

Exploration of Bacterial Bottlenecks and Streptococcus pneumoniae Pathogenesis by CRISPRi-Seq

Author(s): Xue Liu, Jacqueline M Kimmey, Laura Matarazzo, Vincent de Bakker, Laurye Van Maele, Jean-Claude Sirard, Victor Nizet, Jan-Willem Veening
Published in: Cell Host & Microbe, 2021, ISSN 1931-3128
Publisher: Cell Press
DOI: 10.1016/j.chom.2020.10.001

The acquisition of clinically relevant amoxicillin resistance in Streptococcus pneumoniae requires ordered horizontal gene transfer of four loci

Author(s): Paddy S Gibson, Evan Bexkens, Sylvia Zuber, Lauren A Cowley , Jan-Willem Veening
Published in: Plos Pathogens, 2022, ISSN 1553-7366
Publisher: Public Library of Science
DOI: 10.1371/journal.ppat.1010727

2FAST2Q: a general-purpose sequence search and counting program for FASTQ files

Author(s): Afonso M Bravo, Athanasios Typas, Jan-Willem Veening
Published in: PeerJ, 2022, ISSN 2167-8359
Publisher: PeerJ
DOI: 10.7717/peerj.14041

Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events

Author(s): Jun Kurushima, Nathalie Campo, Renske van Raaphorst, Guillaume Cerckel, Patrice Polard, Jan-Willem Veening
Published in: eLife, Issue 9, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.58771

Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification

Author(s): Natalie Bärland, Anne-Stéphanie Rueff, Gonzalo Cebrero, Cedric A J Hutter, Markus A Seeger, Jan-Willem Veening, Camilo Perez
Published in: Science Advances, 2022, ISSN 2375-2548
Publisher: American Association for the Advancement of Science
DOI: 10.1126/sciadv.abm1122

RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae

Author(s): Chryslène Mercy, Adrien Ducret, Jelle Slager, Jean-Pierre Lavergne, Céline Freton, Sathya Narayanan Nagarajan, Pierre Simon Garcia, Marie-Francoise Noirot-Gros, Nelly Dubarry, Julien Nourikyan, Jan-Willem Veening, Christophe Grangeasse
Published in: Nature Microbiology, 2019, ISSN 2058-5276
Publisher: Nature
DOI: 10.1038/s41564-019-0472-z

Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae

Author(s): Lance E. Keller, Anne-Stéphanie Rueff, Jun Kurushima, Jan-Willem Veening
Published in: Genes, Issue 10/5, 2019, Page(s) 394, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10050394

Refining the Pneumococcal Competence Regulon by RNA Sequencing

Author(s): Jelle Slager, Rieza Aprianto, Jan-Willem Veening
Published in: Journal of Bacteriology, Issue 201/13, 2019, ISSN 0021-9193
Publisher: American Society for Microbiology
DOI: 10.1128/jb.00780-18

BactMAP: An R package for integrating, analyzing and visualizing bacterial microscopy data

Author(s): Renske Raaphorst, Morten Kjos, Jan‐Willem Veening
Published in: Molecular Microbiology, Issue 113/1, 2019, Page(s) 297-308, ISSN 0950-382X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14417

Structure of a proton-dependent lipid transporter involved in lipoteichoic acids biosynthesis

Author(s): Bing Zhang, Xue Liu, Elisabeth Lambert, Guillaume Mas, Sebastian Hiller, Jan-Willem Veening, Camilo Perez
Published in: Nature Structural & Molecular Biology, 2020, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-020-0425-5

Gaps in the wall: understanding cell wall biology to tackle amoxicillin resistance in Streptococcus pneumoniae

Author(s): Paddy S Gibson, Jan-Willem Veening
Published in: Curr Opin Microbiol., 2023, ISSN 1369-5274
Publisher: Elsevier BV
DOI: 10.1016/j.mib.2022.102261

Synthetic genetic oscillators demonstrate the functional importance of phenotypic variation in pneumococcal-host interactions

Author(s): Anne-Stéphanie Rueff, Renske van Raaphorst, Surya D Aggarwal, Javier Santos-Moreno, Géraldine Laloux, Yolanda Schaerli, Jeffrey N Weiser, Jan-Willem Veening
Published in: Nature Communications, Issue 14(1):7454, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-43241-y

Competence remodels the pneumococcal cell wall exposing key surface virulence factors that mediate increased host adherence

Author(s): Vikrant Minhas, Arnau Domenech, Dimitra Synefiaridou, Daniel Straume, Max Brendel, Gonzalo Cebrero, Xue Liu, Charlotte Costa, Mara Baldry, Jean-Claude Sirard, Camilo Perez, Nicolas Gisch, Sven Hammerschmidt, Leiv Sigve Håvarstein, Jan-Willem Veening
Published in: Plos Biology, 2023, ISSN 1544-9173
Publisher: Public Library of Science
DOI: 10.1371/journal.pbio.3001990