Preservation of microorganisms by understanding the protective mechanisms of oligosaccharides

Project Information

PREMIUM

Grant agreement ID: 777657

DOI

10.3030/777657

Project closed

EC signature date 21 November 2017

Start date 1 January 2018

End date 30 June 2023

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 634 500,00

EU contribution

€ 634 500,00

Coordinated by

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
France

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (3)

Protocol of samples handling

Protocol of samples handling (rehydration, media composition, freezing, storage…)

Physical properties of LAB media

Report on the glass transitions, viscosity and ice nucleation temperatures of LAB media

Logo and flyers

PREMIUM’s logo and flyers

Websites, patent fillings, videos etc. (1)

Website

PREMIUM’s website

Publications

Peer reviewed articles (29)

Use of Legume Wastewater Extracts on the Storage Stability of Freeze-Dried Lactiplantibacillus plantarum WCFS1

Author(s): Gonçalo Nuno Martins, María Guerrero Sánchez, Angela Daniela Carboni, Stéphanie Cenard, Fernanda Fonseca, Andrea Gómez-Zavaglia, Paula Cristina Castilho
Published in: Food and Bioprocess Technology, 2024, ISSN 1935-5130
Publisher: Springer Pub. Co.,
DOI: 10.1007/s11947-024-03554-2

A critical transfer temperature from slow cooling to cryogenic storage for optimal recovery across a range of cryopreserved mammalian cells

Author(s): Julie Meneghel, Peter Kilbride, Fernanda Fonseca, John Morris
Published in: Cryobiology, Issue 109, 2023, Page(s) 28, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2022.11.088

Insights into the mechanisms of L. salivarius CECT5713 resistance to freeze-dried storage

Author(s): Maria Guerrero Sanchez, Stéphanie Passot, Sarrah Ghorbal, Sonia Campoy, Monica Olivares, Fernanda Fonseca
Published in: Cryobiology, 2023, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2023.104556

Dynamic Modeling of Carnobacterium maltaromaticum CNCM I-3298 Growth and Metabolite Production and Model-Based Process Optimization

Author(s): Cristian Puentes, Amélie Girardeau, Stephanie Passot, Fernanda Fonseca, Ioan-Cristian Trelea
Published in: Foods, Issue 10/8, 2021, Page(s) 1922, ISSN 2304-8158
Publisher: MDPI
DOI: 10.3390/foods10081922

Dataset on the Life Cycle Assessment of fructo- and galacto-oligosaccharides (FOS and GOS) produced by synthesis or hydrolysis.

Author(s): Esteban Gerbino, Camille Quentier, Caroline Pénicaud
Published in: Data in Brief, 2022, ISSN 2352-3409
Publisher: Elsevier BV
DOI: 10.1016/j.dib.2022.108478

Fructose derived oligosaccharides prevent lipid membrane destabilization and DNA conformational alterations during vacuum-drying of Lactobacillus delbrueckii subsp. bulgaricus

Author(s): Nelson Romano, Monica Marro, Maria Marsal, Pablo Loza-Álvarez, Andrea Gomez-Zavaglia
Published in: Food Research International, Issue 143, 2021, Page(s) 110235, ISSN 0963-9969
Publisher: Elsevier BV
DOI: 10.1016/j.foodres.2021.110235

Effect of protective agents on the storage stability of freeze-dried Ligilactobacillus salivarius CECT5713

Author(s): Guerrero Sanchez, M., Passot, S., Campoy, S., Ollivares, M., Fonseca, F.
Published in: Applied Microbiology and Biotechnology, Issue 01757598, 2022, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-022-12201-9

Recent advances in β-galactosidase and fructosyltransferase immobilization technology

Author(s): Maria Micaela Ureta, Gonçalo Nuno Martins, Onofre Figueira, Pedro Filipe Pires, Paula Cristina Castilho, Andrea Gomez-Zavaglia
Published in: Critical Reviews in Food Science and Nutrition, 2020, Page(s) 1-32, ISSN 1040-8398
Publisher: Taylor & Francis
DOI: 10.1080/10408398.2020.1783639

Molecular Simulation: a remarkable tool to study mechanisms of cell membrane preservation in probiotic bacteria

Author(s): T. H. Ferreira, P. Maximiano, M. Ureta, A. Gomez-Zavaglia, and P. N. Simões
Published in: Current Opinion in Food Science, Issue 50, 2023, Page(s) 100985, ISSN 2214-7993
Publisher: Elsevier BV
DOI: 10.1016/j.cofs.2022.100985

The transfer temperature from slow cooling to cryogenic storage is critical for optimal recovery of cryopreserved mammalian cells

Author(s): Peter Kilbride, Julie Meneghel, Fernanda Fonseca, John Morris
Published in: PLOS ONE, Issue 16/11, 2021, Page(s) e0259571, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0259571

Lactic Acid Bacteria in the Production of Traditional Fermented Foods and Beverages of Latin America

Author(s): Angela D. Carboni, Gonçalo N. Martins, Andrea Gómez-Zavaglia, Paula C. Castilho
Published in: Fermentation, Issue 9, 2023, Page(s) 315, ISSN 2311-5637
Publisher: MDPI
DOI: 10.3390/fermentation9040315

Membrane lipid composition of Carnobacterium maltaromaticum CNCM I-3298, a highly cryoresistant lactic bacterium

Author(s): HP. Ta, C. Clarisse, E. Maes, N. Yamakawa, Y. Guérardel, F. Krzewinski, W. Zarzycka, D. Touboul, A. Girardeau, F. Fonseca, A. Kermarrec, M. Viau, A. Riaublanc, MH. Ropers
Published in: Chemistry and Physics of Lipids, 2023, ISSN 0009-3084
Publisher: Elsevier BV
DOI: 10.1016/j.chemphyslip.2023.105326

Chickpeas’ cooking wastewater as an alternative source of galacto-oligosaccharides for improving the freeze-dried resistance of lactic acid bacteria

Author(s): Passot S., Cenard S., Lieben P., Ghorbal S., Martins G.N., Castilho P.C., Fonseca F.
Published in: ACS Food Science & Technology, 2024, ISSN 2692-1944
Publisher: American Chemical Society Publications
DOI: 10.1021/acsfoodscitech.4c00120

Dataset on the life cycle assessment of the production of stabilized lactic acid bacteria

Author(s): Maite Gagneten, Camille Quentier, Stéphanie Passot, Stéphanie Cenard, Fernanda Fonseca, Caroline Pénicaud
Published in: Data in Brief, Issue 60, 2025, Page(s) 111541, ISSN 2352-3409
Publisher: Elsevier BV
DOI: 10.1016/j.dib.2025.111541

Infrared spectroscopy as an alternative methodology to evaluate the effect of structural features on the physical-chemical properties of inulins

Author(s): Nelson Romano, Cuauhtémoc Araujo-Andrade, Javier Lecot, Pablo Mobili, Andrea Gómez-Zavaglia
Published in: Food Research International, Issue 109, 2018, Page(s) 223-231, ISSN 0963-9969
Publisher: Elsevier BV
DOI: 10.1016/j.foodres.2018.04.032

Development and characterization of iron-pectin beads as a novel system for iron delivery to intestinal cells

Author(s): F. Ghibaudo, E. Gerbino, A.A. Hugo, M.G. Simões, P. Alves, B.F.O. Costa, V. Campo Dallˊ Orto, A. Gómez-Zavaglia, P.N. Simões
Published in: Colloids and Surfaces B: Biointerfaces, Issue 170, 2018, Page(s) 538-543, ISSN 0927-7765
Publisher: Elsevier BV
DOI: 10.1016/j.colsurfb.2018.06.052

Physical events occurring during the cryopreservation of immortalized human T cells

Author(s): Julie Meneghel, Peter Kilbride, John G. Morris, Fernanda Fonseca
Published in: PLOS ONE, Issue 14/5, 2019, Page(s) e0217304, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0217304

Long term stability and interaction with epithelial cells of freeze-dried pH-responsive liposomes functionalized with cholesterol-poly(acrylic acid)

Author(s): M.G. Simões, A. Hugo, P. Alves, P.F. Pérez, A. Gómez-Zavaglia, P.N. Simões
Published in: Colloids and Surfaces B: Biointerfaces, Issue 164, 2018, Page(s) 50-57, ISSN 0927-7765
Publisher: Elsevier BV
DOI: 10.1016/j.colsurfb.2018.01.018

Technological Aspects of the Production of Fructo and Galacto-Oligosaccharides. Enzymatic Synthesis and Hydrolysis

Author(s): Gonçalo N. Martins, Maria Micaela Ureta, E. Elizabeth Tymczyszyn, Paula C. Castilho, Andrea Gomez-Zavaglia
Published in: Frontiers in Nutrition, Issue 6, 2019, ISSN 2296-861X
Publisher: Frontiers
DOI: 10.3389/fnut.2019.00078

Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Author(s): Fernanda Fonseca, Caroline Pénicaud, E. Elizabeth Tymczyszyn, Andrea Gómez-Zavaglia, Stéphanie Passot
Published in: Applied Microbiology and Biotechnology, 2019, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-019-10002-1

Influence of culture conditions on the technological properties of Carnobacterium maltaromaticum CNCM I‐3298 starters

Author(s): A. Girardeau, C. Puentes, S. Keravec, P. Peteuil, I.C. Trelea, F. Fonseca
Published in: Journal of Applied Microbiology, Issue 126/5, 2019, Page(s) 1468-1479, ISSN 1364-5072
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/jam.14223

Nutritional and technological properties of a quinoa (Chenopodium quinoa Willd.) spray-dried powdered extract

Author(s): Nelson Romano, María Micaela Ureta, María Guerrero-Sánchez, Andrea Gómez-Zavaglia
Published in: Food Research International, Issue 129, 2020, Page(s) 108884, ISSN 0963-9969
Publisher: Elsevier BV
DOI: 10.1016/j.foodres.2019.108884

Mechanistic study of the differences in lactic acid bacteria resistance to freeze- or spray-drying and storage

Author(s): Gagneten M., Passot S., Cenard S., Lieben P., Ghorbal S., Schebor C., Fonseca F.
Published in: Applied Microbiology and Biotechnology, 2024, ISSN 1432-0614
Publisher: Springer
DOI: 10.1007/s00253-024-13186-3

Chickpeas’ and lentils' soaking and cooking wastewaters repurposed for growing lactic acid bacteria.

Author(s): Martins GN, Carboni AD, Hugo AA, Castilho PC, Gómez-Zavaglia A.
Published in: Foods, 2023, ISSN 2304-8158
Publisher: MDPI
DOI: 10.3390/foods12122324

Multi-objective optimization of frozen and freeze-dried Lactobacillus delbrueckii subsp. bulgaricus CFL1 production via the modification of fermentation conditions

Author(s): Maria de L TovillaCoutiño; Stéphanie Passot; Ioan-Cristian Trelea; Marie-Hélène Ropers; Yann Gohon; Fernanda Fonseca
Published in: Journal of Applied Microbiology, Issue 3, 2023, ISSN 0021-8847
Publisher: Oxford University Press
DOI: 10.1093/jambio/lxad003

Joining environmental impacts and product quality in Life Cycle Assessment: The case of the production and storage of lactic acid bacteria concentrates

Author(s): Maite Gagneten, Camille Quentier, Stéphanie Passot, Stéphanie Cenard, Fernanda Fonseca, Caroline Pénicaud
Published in: Cleaner Environmental Systems, Issue 15, 2024, Page(s) 100245, ISSN 2666-7894
Publisher: Elsevier BV
DOI: 10.1016/j.cesys.2024.100245

Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

Author(s): M. Guerrero Sanchez; S. Passot; S. Campoy; M. Olivares; F. Fonseca
Published in: Applied Microbiology and Biotechnology, Issue 6, 2021, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-021-11694-0

Insights into lactic acid bacteria cryoresistance using FTIR microspectroscopy

Author(s): Amélie Girardeau; Stéphanie Passot; Julie Meneghel; Stéphanie Cenard; Pascale Lieben; Ioan-Cristian Trelea; Fernanda Fonseca
Published in: Analytical and Bioanalytical Chemistry, Issue 5, 2022, Page(s) 1425-1443, ISSN 1618-2642
Publisher: Springer Verlag
DOI: 10.1007/s00216-021-03774-x

Influence of Thermal Treatment and Granulometry on Physicochemical, Techno-Functional and Nutritional Properties of Lentil Flours

Author(s): Angela Daniela Carboni, Gonçalo Nuno Martins, Paula Cristina Castilho, María Cecilia Puppo, Cristina Ferrero
Published in: Foods, Issue 13, 2024, Page(s) 2744, ISSN 2304-8158
Publisher: MDPI
DOI: 10.3390/foods13172744

Conference proceedings (10)

Methodological approach towards environmentally friendly processes for preserving lactic acid bacteria.Oral presentation

Author(s): Caroline Pénicaud, Bruno Perret, Stéphanie Passot, Camille Quentier, Fernanda Fonseca.
Published in: CBL 2019 - 22. Colloque du Club des Bactéries Lactiques-12-14 June, 2019
Publisher: Université de Caen

The Critical Thermal Range For Controlled Rate Cooling For Mammalian Cell Cryopreservation

Author(s): Julie Meneghel, Peter Kilbride, Fernanda Fonseca, John Morris
Published in: Cryobiology, Issue 91, 2019, Page(s) 175, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2019.10.118

Preserving bacteria with oligosaccharides and eco-friendly processes (Premium)

Author(s): Andrea Gomez-Zavaglia, Stéphanie Passot, Caroline Pénicaud, Paula Castilho, Pedro Nuno Simões, Marie Hélène Ropers, Bruno Perret, Alberto Garach Domech, Pablo Loza-Alvarez, John Morris, Peter Kilbride, Sophie Keravec, Fernanda Fonseca
Published in: Cryobiology, Issue 85, 2018, Page(s) 172-173, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2018.10.203

Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation. Invited lecture

Author(s): Fernanda Fonseca, Julie Meneghel, Peter Kilbride, Stéphanie Passot, G. John Morris
Published in: 9. International Symposium on Lyophilization of Pharmaceuticals ISLFD, Issue 15, 2019
Publisher: Université Paris-Saclay

Avancées récentes sur la production et la stabilisation de bactéries lactiques à haute fonctionnalité

Author(s): Stéphanie Passot; Catherine Beal; Fernanda Fonseca
Published in: CBL 2019 - 22. Colloque du Club des Bactéries Lactiques, Jun 2019, Caen, France, Issue 6, 2019
Publisher: CBL proceedings

Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation

Author(s): Fernanda Fonseca; Julie Meneghel; Peter Kilbride; Stéphanie Passot; John Morris, G.
Published in: HAL, Issue 1, 2019
Publisher: Université Paris-Saclay

Complementary Analytical Approaches Improving Knowledge On Lactic Acid Bacteria Cryoresistance

Author(s): Amelie Girardeau, Julie Meneghel, Ioan-Cristian Trelea, Stephanie Passot, Fernanda Fonseca
Published in: Cryobiology, Issue 91, 2019, Page(s) 166-167, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2019.10.086

Development Of A Simplified Lipid Model Of Bacterial Membrane To Investigate Specific Interactions With Oligosaccharides

Author(s): Fernanda Fonseca, Michele Da Silva Pinto, Julie Meneghel, Pascale Lieben, Stephanie Passot, Yann Gohon, Marie Helene Ropers
Published in: Cryobiology, Issue 91, 2019, Page(s) 173, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2019.10.109

Differential scanning calorimetry applied to the preservation of bacteria and mammalian cells

Author(s): Fonseca, Fernanda; Passot, Stéphanie
Published in: https://hal.science/hal-04395225, Issue 19, 2023
Publisher: Université Paris-Saclay

Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation. Invited lecture

Author(s): Fernanda Fonseca, Julie Meneghel, Peter Kilbride, Stéphanie Passot, G. John Morris
Published in: . 9. International Symposium on Lyophilization of Pharmaceuticals ISLFD, 2019
Publisher: Ghent University

Other (3)

Website

Author(s): PREMIUM
Published in: 2018
Publisher: INRAE

Flyer

Author(s): PREMIUM
Published in: 2020
Publisher: INRAE

Promotional video

Author(s): PREMIUM
Published in: 2020
Publisher: INRAE

Thesis and dissertations (4)

Improving the cryo-resistance of a lactic acid bacterium by modulating fermentation and stabilization conditions: consequences on the properties of the lipid membrane

Author(s): Tovilla Coutino, Maria de Lourdes
Published in: https://pastel.hal.science/tel-03942745, Issue 11, 2022
Publisher: HAL

Study of C. maltaromaticum CNCM I-3298 cellular characteristics to improve performance of biological time-temperature integrators and gain more insight into lactic acid bacteria cryoresistance

Author(s): Amélie Girardeau
Published in: 2021
Publisher: Université Paris-Saclay, INRAE, AgroParisTech, UMR SayFood

Improvements of Ligilatobacillus salivarius CECT5713 resistance to freeze-drying and storage modifying the production conditions and studying the mechanisms involved

Author(s): Maria Guerrero Sanchez
Published in: 2022
Publisher: Universidad de Granada

Improving the cryo-resistance of a lactic acid bacterium by modulating fermentation and stabilization conditions: consequences on the properties of the lipid membrane

Author(s): Maria de Lourdes Tovilla-Coutiño
Published in: 2022
Publisher: Université Paris-Saclay, INRAE, AgroParisTech, UMR SayFood

Book chapters (3)

Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties

Author(s): Fernanda Fonseca, Amélie Girardeau, Stéphanie Passot
Published in: Cryopreservation and Freeze-Drying Protocols, Issue 2180, 2021, Page(s) 703-719, ISBN 978-1-0716-0782-4
Publisher: Springer US
DOI: 10.1007/978-1-0716-0783-1_38

Scale-Up of Layer-by-Layer Microencapsulation and Freeze-Drying of Probiotic Bacteria

Author(s): Nelson Romano, Gina Tavares, Stéphanie Passot, Fernanda Fonseca, Sonia Campoy, Maria Guerrero, Patrícia Alves, Patrícia Coimbra, Andrea Gomez-Zavaglia, Pedro Nuno Simões
Published in: Methods and Protocols in Food Science, Basic Protocols in Encapsulation of Food Ingredients, 2024, Page(s) 57-67
Publisher: Springer US
DOI: 10.1007/978-1-0716-4148-4_7

Application of Lactic Acid Bacteria in Time-Temperature Integrators

Author(s): Amélie Girardeau, Vanessa Biscola, Sophie Keravec, Georges Corrieu, Fernanda Fonseca
Published in: Lactic Acid Bacteria - A Functional Approach, 2020, Page(s) 241-260, ISBN 9780-429422591
Publisher: CRC Press
DOI: 10.1201/9780429422591-14