Mechanistic analysis of DNA damage signaling and bypass upon replication of damaged DNA template in human cells.

Projektinformationen

DNAcheck

ID Finanzhilfevereinbarung: 794054

DOI

10.3030/794054

Projekt abgeschlossen

EK-Unterschriftsdatum 15 März 2018

Startdatum 1 Oktober 2018

Enddatum 30 September 2020

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten

€ 170 121,60

EU-Beitrag

€ 170 121,60

Koordiniert durch

UNIVERSIDAD DE SEVILLA
Spain

Periodic Reporting for period 1 - DNAcheck (Mechanistic analysis of DNA damage signaling and bypass upon replication of damaged DNA template in human cells.)

Berichtszeitraum: 2018-10-01 bis 2020-09-30

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

The genetic information encoded by DNA is constantly threatened by endogenous or exogenous sources of damage. To safeguard the genome against these insults, cells have evolved DNA damage checkpoints that sense the presence of damaged DNA, block cell cycle progression and ensure that DNA is fully repaired before resuming the cell cycle. However, in many cases, unrepaired lesions remain in the DNA when cells enter S-phase. In this scenario, cells employ DNA damage tolerance mechanisms to complete genome replication and prevent fork breakage. Importantly, these pathways are not restricted to the site of stalling but can also function behind the fork at single-stranded(ss)DNA gaps originated by re-priming of DNA synthesis downstream of lesions.
While it is well known that the accumulation of single-stranded DNA (ssDNA) is the signal that riggers the checkpoint response, it is less clear how and where ssDNA actually arises. Generally, it is assumed to accumulate at stalled replication forks by an uncoupling between replicative helicase and polymerase movement. However, recent evidence found in budding yeast support a model where, in response to polymerase-blocking lesions, replication forks do not stall but recover efficiently by re-priming downstream of the lesions, thus leaving ssDNA gaps behind the fork. It is currently unknown whether this model also applies to vertebrate cells. Hence, this project aimed to address the fundamental question of how DNA damage is sensed during genome replication in human cells.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

In the course of this project, we have identified a remarkable mechanism that promote the robust activation of the replication checkpoint in human cell lines, which involves the action of different nucleases at ssDNA gaps generated behind replication forks in the presence of lesions on the DNA template. Remarkably, this mechanism did not apply when lesion-less replication stress was induced. Thus, these results have shown further evidence of a dichotomy between the cellular response to damaged DNA template versus replisome problems.
The results of the project have been presented to the scientific community at multiple conferences and workshops and are expected to be published within a year.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

The project results have helped to better understand how human cells sense and deal with replicative stress, a process that is highly relevant to human disease such as cancer. Interestingly, deregulation of nuclease levels has been reported in different types of cancer. Hence, these nucleases may offer a target for cancer therapeutic development.

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