Descrizione del progetto
Svelare la neurobiologia dell’alimentazione incontrollata indotta dallo stress
I disturbi alimentari e l’obesità minacciano la salute in modi diversi. Il disturbo da alimentazione incontrollata è stato collegato a disturbi dei circuiti neurali, ma sono necessarie ulteriori indagini per trovare opzioni di trattamento efficaci per questa condizione. Il progetto ReCoDE, finanziato dall’UE, si concentrerà sul modo in cui lo stress altera la forza delle connessioni cerebrali coinvolte nel processo decisionale e sul modo in cui può innescare un comportamento alimentare impulsivo. Inoltre, cercherà modi per rimodellare questo processo attraverso la manipolazione specifica dell’attività cerebrale. Verranno applicate l’elettrofisiologia, l’optogenetica e il tracciamento neurale, per scoprire se l’alimentazione incontrollata si verifica a causa di cambiamenti nel controllo della corteccia prefrontale sui circuiti dell’ipotalamo laterale che regolano l’assunzione di cibo. I risultati potrebbero rivelarsi promettenti per il trattamento futuro dei disturbi alimentari.
Obiettivo
Obesity and eating disorders are critical problems in society. Many patients with these brain diseases cope with stress by ravenous food intake (binge eating), which engenders new stress and maintains the pathology. Evidence-based treatments for this are urgently needed, but their implementation is hindered by a knowledge gap on: (i) which stress-driven neural disruptions cause binge eating, and (ii) whether these neural circuit changes can be normalized for therapeutic gain.
Studies in humans and rodents link binge eating to dysfunction of the prefrontal cortex (PFC), a brain region orchestrating the stress response. However, it is unknown how effects of stress on PFC output cause binge eating. The PFC prominently innervates the lateral hypothalamus (LHA), a region with a crucial role in managing food intake, yet little is known about the function of PFC regulation of the LHA. I predict that stress-induced binge eating requires a functional reorganization of prefrontal cortical control over lateral hypothalamus feeding circuits, and that this control can be restored to limit binge eating.
I propose a cutting-edge threefold strategy to address these hypotheses in mouse models:
1. I will unravel the make-up of PFC-LHA circuitry, combining electrophysiology, optogenetics and neural tracing. I will assess how stress functionally alters this complex network.
2. I will determine the concurrent activity at multiple sites within PFC-LHA circuitry as mice engage in stress-driven binge eating, using fiber photometric calcium recordings.
3. I will assess if normalizing stress-altered PFC-LHA synapses rebalances this circuitry in vivo and limits binge eating. For this I will combine optogenetic plasticity protocols, with fiber photometric measurements in freely moving mice.
Overall, this challenging project aims to unravel the unclear neurobiology of stress-induced binge eating. If successful, this would provide a key advance in understanding binge eating pathologies.
Campo scientifico
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
ERC-STG - Starting GrantIstituzione ospitante
3584 CX Utrecht
Paesi Bassi