Synthetic biology of carbohydrate-binding proteins: engineering protein-carbohydrate interactions for diagnostics and cell targeting

Project Information

synBIOcarb

Grant agreement ID: 814029

DOI

10.3030/814029

Project closed

EC signature date 20 August 2018

Start date 1 October 2018

End date 31 March 2023

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 4 093 660,08

EU contribution

€ 4 093 660,08

Coordinated by

UNIVERSITY OF LEEDS
United Kingdom

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Other (1)

ESR project vacancies advertised

Websites, patent fillings, videos etc. (6)

E-newsletter 2 published

Enewsletter 2 published

International Conference on Synthetic Glycobiology
E-newsletter 1 published

Enewsletter 1 published

E-newsletter3 published

Enewsletter3 published

synBIOcarb website running (public section)

synBIOcarb website running public section

Report on one day contact meeting with industry

Publications

Other (2)

Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids

Author(s): Sarah Wehrum; Daniel J Williamson; Lina Siukstaite; Thomas R Branson; Taras Sych; Josef Madl; Gemma C Wildsmith; Wenyue Dai; James F Ross; Maren Thomsen; Michael E Webb; Winfried Römer; W Bruce Turnbull
Published in: ChemRxiv, 2021
Publisher: Cambridge University Press
DOI: 10.26434/chemrxiv-2021-j9stg

A pore-forming β-trefoil lectin with specificity for the tumor-related glycosphingolipid Gb3

Author(s): Simona Notova, François Bonnardel, Francesca Rosato, Lina Siukstaite, Jessica Schwaiger, Nicolai Bovin, Annabelle Varrot, Winfried Römer, Frédérique Lisacek, Anne Imberty
Published in: BioRxiv, 2022
Publisher: Cold Harbour Spring Laboratory
DOI: 10.1101/2022.02.10.479907

Peer reviewed articles (27)

Tuning specificity and topology of lectins through synthetic biology

Author(s): Simona Notova, Anne Imberty
Published in: Current Opinion on Chemical Biology, Issue 73, 2023, Page(s) 102275, ISSN 1367-5931
Publisher: Elsevier BV
DOI: 10.1016/j.cbpa.2023.102275

The Two Sweet Sides of Janus Lectin Drive Crosslinking of Liposomes to Cancer Cells and Material Uptake

Author(s): Lina Siukstaite, Francesca Rosato, Anna Mitrovic, Peter Fritz Müller, Katharina Kraus, Simona Notova, Anne Imberty, Winfried Römer
Published in: Toxins, Issue 13/11, 2021, Page(s) 792, ISSN 2072-6651
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins13110792

Structural Diversities of Lectins Binding to the Glycosphingolipid Gb3

Author(s): Lina Siukstaite, Anne Imberty, Winfried Römer
Published in: Frontiers in Molecular Biosciences, Issue 8, 2021, ISSN 2296-889X
Publisher: Frontiers in Molecular Biosciences
DOI: 10.3389/fmolb.2021.704685

Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray

Author(s): Tomas Berto, Aniko Bertokova, Eduard Jane, Michal Hires, Juvissan Aguedo, Maria Potocarova, Ludovit Lukac, Alica Vikartovska, Peter Kasak, Lubor Borsig and Jan Tkac
Published in: Frontiers in Oncology, Issue 11, 2021, Page(s) 735338, ISSN 2234-943X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fonc.2021.735338

Structure and engineering of tandem repeat lectins

Author(s): Simona Notova, François Bonnardel, Frédérique Lisacek, Annabelle Varrot, Anne Imberty
Published in: Current Opinion in Structural Biology, Issue 62, 2020, Page(s) 39-47, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2019.11.006

Electrochemical Impedance Spectroscopy on 2D Nanomaterial MXene Modified Interfaces: Application as a Characterization and Transducing Tool

Author(s): Juvissan Aguedo, Lenka Lorencova, Marek Barath, Pavol Farkas and Jan Tkac
Published in: Chemosensors, Issue 8/4, 2020, Page(s) 127, ISSN 2227-9040
Publisher: MDPI
DOI: 10.3390/chemosensors8040127

Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids

Author(s): Sarah Wehrum, Lina Siukstaite, Daniel J. Williamson, Thomas R. Branson, Taras Sych, Josef Madl, Gemma C. Wildsmith, Wenyue Dai, Erik Kempmann, James F. Ross, Maren Thomsen, Michael E. Webb, Winfried Römer, and W. Bruce Turnbull
Published in: ACS Synthetic Biology, Issue 11 (12), 2022, Page(s) 3929–3938, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.2c00266

Genetic code expansion in E. coli enables production of a functional‘ready-to-click’ T cell receptor-specific scFv

Author(s): Rajeev Pasupuleti, Francesca Rosato, Dajana Kolanovic, Olga N. Makshakova, Winfried Römer and Birgit Wiltschi
Published in: New Biotechnology, Issue 76, 2023, Page(s) 127-137, ISSN 1871-6784
Publisher: Elsevier BV
DOI: 10.1016/j.nbt.2023.05.007

Building an Artificial Plant Cell Wall on a Lipid Bilayer by Assembling Polysaccharides and Engineered Proteins

Author(s): Simona Notova; Nathan Cannac; Luca Rabagliati; Maeva Touzard; Josselin Mante; Yotam Navon; Liliane Coche-Guérente; Olivier Lerouxel; Laurent Heux; Anne Imberty
Published in: ACS Synthetic Biology, Issue 11/10, 2022, Page(s) 3516–3528, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.2c00404

Extending Janus lectins architecture: Characterization and application to protocells

Author(s): Simona Notova, Lina Siukstaite, Francesca Rosato, Federica Vena, Aymeric Audfray, Nicolai Bovin, Ludovic Landemarre, Winfried Römer, Anne Imberty
Published in: Computational and Structural Biotechnology Journal, Issue 20, 2022, Page(s) 6108-6119, ISSN 2001-0370
Publisher: Elsevier
DOI: 10.1016/j.csbj.2022.11.005

Homo-and Heterovalent Neoglycoproteins as Ligands for Bacterial Lectins

Author(s): David Goyard, Benoît Roubinet, Federica Vena, Ludovic Landemarre, Olivier Renaudet
Published in: ChemPlusChem, Issue 87/2, 2022, ISSN 2192-6506
Publisher: Wiley-VCH Verlag
DOI: 10.1002/cplu.202100481

Microbial carbohydrate-binding toxins – From etiology to biotechnological application

Author(s): Natalia Danielewicz; Francesca Rosato; Wenyue Dai; Winfried Römer; W. Bruce Turnbull; Juergen Mairhofer
Published in: Biotechnology Advances, Issue 59, 2022, ISSN 0734-9750
Publisher: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107951

A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

Author(s): Paras H. Kundalia , Lucia Pažitná, Kristína Kianičková, Eduard Jáné, Lenka Lorencová, Jaroslav Katrlík
Published in: Sensors, Issue 23(12), 2023, ISSN 1424-8220
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s23125362

Enzymatic Glyco-Modification of Synthetic Membrane Systems

Author(s): Dylan Jabeguero, Lina Siukstaite, Chunyue Wang, Anna Mitrovic, Serge Pérez, Olga Makshakova, Ralf P. Richter, Winfried Römer and Christelle Breton
Published in: Biomolecules, Issue 13(2), 2023, Page(s) 335, ISSN 2218-273X
Publisher: MDPI
DOI: 10.3390/biom13020335

Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy

Author(s): Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Published in: ACS Omega, Issue 8 (17), 2023, Page(s) 15406–15421, ISSN 2470-1343
Publisher: American Chemical Society
DOI: 10.1021/acsomega.3c00667

Targeted delivery of oligonucleotides using multivalent protein–carbohydrate interactions

Author(s): Vajinder Kumar and W. Bruce Turnbull
Published in: Chemical Society Reviews, Issue 52, 2023, Page(s) 1273-1287, ISSN 1460-4744
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2cs00788f

Neutralizing the Impact of the Virulence Factor LecA from <i>Pseudomonas aeruginosa</i> on Human Cells with New Glycomimetic Inhibitors

Author(s): Eva Zahorska; Francesca Rosato; Kai Stober; Sakonwan Kuhaudomlarp; Joscha Meiers; Dirk Hauck; Dorina Reith; Emilie Gillon; Katharina Rox; Anne Imberty; Winfried Römer; Alexander Titz
Published in: Angewandte Chemie International Edition, Issue 62, 2023, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202215535

The Lectin LecA Sensitizes the Human Stretch-Activated Channel TREK-1 but Not Piezo1 and Binds Selectively to Cardiac Non-myocytes

Author(s): Elisa Darkow, Eva A. Rog-Zielinska, Josef Madl, Annette Brandel, Lina Siukstaite, Ramin Omidvar, Peter Kohl, Ursula Ravens, Winfried Römer, Rémi Peyronnet
Published in: Frontiers in Physiology, Issue 11, 2020, ISSN 1664-042X
Publisher: Frontiers Research Foundation
DOI: 10.3389/fphys.2020.00457

In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli

Author(s): Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Published in: Toxins, 2022, ISSN 2072-6651
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins14060396

MXene as a novel cartridge for N-glycan enrichment

Author(s): Juvissan Aguedo, Zuzana Pakanova, Lenka Lorencova, Marek Nemcovic, Peter Kasak, Marek Barath, Pavol Farkas, JanTkac
Published in: Analytica Chimica Acta, Issue 00032670, 2022, Page(s) Volume 1234, ISSN 0003-2670
Publisher: Elsevier BV
DOI: 10.1016/j.aca.2022.340512

A bispecific, crosslinking lectibody activates cytotoxic T cells and induces cancer cell death

Author(s): Francesca Rosato, Rajeev Pasupuleti, Jana Tomisch, Ana Valeria Meléndez, Dajana Kolanovic, Olga N. Makshakova, Birgit Wiltschi & Winfried Römer
Published in: Journal of Translational Medicine, Issue 20/578, 2022, ISSN 1479-5876
Publisher: BioMed Central
DOI: 10.1186/s12967-022-03794-w

Stabilization of membrane topologies by proteinaceous remorin scaffolds

Author(s): Chao Su, Marta Rodriguez-Franco, Beatrice Lace, Nils Nebel, Casandra Hernandez-Reyes, Pengbo Liang, Eija Schulze, Evgeny V Mymrikov, Nikolas M Gross, Julian Knerr, Hong Wang, Lina Siukstaite, Jean Keller, Cyril Libourel, Alexandra AM Fischer, Katharina E Gabor, Eric Mark, Claudia Popp, Carola Hunte, Wilfried Weber, Petra Wendler, Thomas Stanislas, Pierre-Marc Delaux, Oliver Einsle, Robert Grosse,
Published in: Nature Comunications, Issue 14/323, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-35976-5

Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells

Author(s): Ana Valeria Meléndez, Rubí M.-H. Velasco Cárdenas, Simon Lagies, Juliane Strietz, Lina Siukstaite, Oliver S. Thomas, Jana Tomisch, Wilfried Weber, Bernd Kammerer, Winfried Römer, Susana Minguet
Published in: Cellular and Molecular Life Sciences, Issue 1420682X, 2022, ISSN 1420-682X
Publisher: Birkhauser Verlag
DOI: 10.1007/s00018-022-04524-7

Piggybacking on the Cholera Toxin: Identification of a CTB-Binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons.

Author(s): Matthew R. Balmforth; Jessica L. Haigh; Vajinder Kumar; Vajinder Kumar; Wenyue Dai; Christian Tiede; Darren C. Tomlinson; Jim Deuchars; Michael E. Webb; W. Bruce Turnbull
Published in: Bioconjugate Chemistry, Issue 32 (10), 2021, Page(s) 2205–2212, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.1c00373

The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3

Author(s): Simona Notova; François Bonnardel; Francesca Rosato; Lina Siukstaite; Jessica Schwaiger; Jia Hui Lim; Nicolai Bovin; Annabelle Varrot; Yu Ogawa; Winfried Römer; Frédérique Lisacek; Anne Imberty
Published in: Communications Biology, Issue 5/954, 2022, ISSN 2399-3642
Publisher: Nature Publising Group
DOI: 10.1038/s42003-022-03869-w

Polyvalent Transition-State Analogues of Sialyl Substrates Strongly Inhibit Bacterial Sialidases

Author(s): Coralie Assailly, Clarisse Bridot, Dr. Amélie Saumonneau, Paul Lottin, Dr. Benoit Roubinet, Dr. Eva-Maria Krammer, Francesca François, Federica Vena, Dr. Ludovic Landemarre, Dr. Dimitri Alvarez Dorta, Dr. David Deniaud, Dr. Cyrille Grandjean, Dr. Charles Tellier, Dr. Sagrario Pascual, Dr. Véronique Montembault, Dr. Laurent Fontaine, Dr. Franck Daligault, Dr. Julie Bouckaert, Dr. Sébastien G. G
Published in: Chemistry – A European Journal, Issue 27, 2021, Page(s) 3142-3150, ISSN 1521-3765
Publisher: Wiley-VCH
DOI: 10.1002/chem.202004672

Involvement of N‐glycans in binding of Photorhabdus luminescens Tc toxin

Author(s): Peter Njenga Ng'ang'a, Lina Siukstaite, Alexander E. Lang, Hans Bakker, Winfried Römer, Klaus Aktories, Gudula Schmidt
Published in: Cellular Microbiology, Issue Monthly, 2021, ISSN 1462-5814
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/cmi.13326