Synthetic biology of carbohydrate-binding proteins: engineering protein-carbohydrate interactions for diagnostics and cell targeting

Información del proyecto

synBIOcarb

Identificador del acuerdo de subvención: 814029

DOI

10.3030/814029

Proyecto cerrado

Fecha de la firma de la CE 20 Agosto 2018

Fecha de inicio 1 Octubre 2018

Fecha de finalización 31 Marzo 2023

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 4 093 660,08

Aportación de la UE

€ 4 093 660,08

Coordinado por

UNIVERSITY OF LEEDS
United Kingdom

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Other (1)

ESR project vacancies advertised

Websites, patent fillings, videos etc. (6)

E-newsletter 2 published

Enewsletter 2 published

International Conference on Synthetic Glycobiology
E-newsletter 1 published

Enewsletter 1 published

E-newsletter3 published

Enewsletter3 published

synBIOcarb website running (public section)

synBIOcarb website running public section

Report on one day contact meeting with industry

Publicaciones

Other (2)

Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids

Autores: Sarah Wehrum; Daniel J Williamson; Lina Siukstaite; Thomas R Branson; Taras Sych; Josef Madl; Gemma C Wildsmith; Wenyue Dai; James F Ross; Maren Thomsen; Michael E Webb; Winfried Römer; W Bruce Turnbull
Publicado en: ChemRxiv, 2021
Editor: Cambridge University Press
DOI: 10.26434/chemrxiv-2021-j9stg

A pore-forming β-trefoil lectin with specificity for the tumor-related glycosphingolipid Gb3

Autores: Simona Notova, François Bonnardel, Francesca Rosato, Lina Siukstaite, Jessica Schwaiger, Nicolai Bovin, Annabelle Varrot, Winfried Römer, Frédérique Lisacek, Anne Imberty
Publicado en: BioRxiv, 2022
Editor: Cold Harbour Spring Laboratory
DOI: 10.1101/2022.02.10.479907

Peer reviewed articles (27)

Tuning specificity and topology of lectins through synthetic biology

Autores: Simona Notova, Anne Imberty
Publicado en: Current Opinion on Chemical Biology, Edición 73, 2023, Página(s) 102275, ISSN 1367-5931
Editor: Elsevier BV
DOI: 10.1016/j.cbpa.2023.102275

The Two Sweet Sides of Janus Lectin Drive Crosslinking of Liposomes to Cancer Cells and Material Uptake

Autores: Lina Siukstaite, Francesca Rosato, Anna Mitrovic, Peter Fritz Müller, Katharina Kraus, Simona Notova, Anne Imberty, Winfried Römer
Publicado en: Toxins, Edición 13/11, 2021, Página(s) 792, ISSN 2072-6651
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins13110792

Structural Diversities of Lectins Binding to the Glycosphingolipid Gb3

Autores: Lina Siukstaite, Anne Imberty, Winfried Römer
Publicado en: Frontiers in Molecular Biosciences, Edición 8, 2021, ISSN 2296-889X
Editor: Frontiers in Molecular Biosciences
DOI: 10.3389/fmolb.2021.704685

Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray

Autores: Tomas Berto, Aniko Bertokova, Eduard Jane, Michal Hires, Juvissan Aguedo, Maria Potocarova, Ludovit Lukac, Alica Vikartovska, Peter Kasak, Lubor Borsig and Jan Tkac
Publicado en: Frontiers in Oncology, Edición 11, 2021, Página(s) 735338, ISSN 2234-943X
Editor: Frontiers Media S. A.
DOI: 10.3389/fonc.2021.735338

Structure and engineering of tandem repeat lectins

Autores: Simona Notova, François Bonnardel, Frédérique Lisacek, Annabelle Varrot, Anne Imberty
Publicado en: Current Opinion in Structural Biology, Edición 62, 2020, Página(s) 39-47, ISSN 0959-440X
Editor: Elsevier BV
DOI: 10.1016/j.sbi.2019.11.006

Electrochemical Impedance Spectroscopy on 2D Nanomaterial MXene Modified Interfaces: Application as a Characterization and Transducing Tool

Autores: Juvissan Aguedo, Lenka Lorencova, Marek Barath, Pavol Farkas and Jan Tkac
Publicado en: Chemosensors, Edición 8/4, 2020, Página(s) 127, ISSN 2227-9040
Editor: MDPI
DOI: 10.3390/chemosensors8040127

Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids

Autores: Sarah Wehrum, Lina Siukstaite, Daniel J. Williamson, Thomas R. Branson, Taras Sych, Josef Madl, Gemma C. Wildsmith, Wenyue Dai, Erik Kempmann, James F. Ross, Maren Thomsen, Michael E. Webb, Winfried Römer, and W. Bruce Turnbull
Publicado en: ACS Synthetic Biology, Edición 11 (12), 2022, Página(s) 3929–3938, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.2c00266

Genetic code expansion in E. coli enables production of a functional‘ready-to-click’ T cell receptor-specific scFv

Autores: Rajeev Pasupuleti, Francesca Rosato, Dajana Kolanovic, Olga N. Makshakova, Winfried Römer and Birgit Wiltschi
Publicado en: New Biotechnology, Edición 76, 2023, Página(s) 127-137, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2023.05.007

Building an Artificial Plant Cell Wall on a Lipid Bilayer by Assembling Polysaccharides and Engineered Proteins

Autores: Simona Notova; Nathan Cannac; Luca Rabagliati; Maeva Touzard; Josselin Mante; Yotam Navon; Liliane Coche-Guérente; Olivier Lerouxel; Laurent Heux; Anne Imberty
Publicado en: ACS Synthetic Biology, Edición 11/10, 2022, Página(s) 3516–3528, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.2c00404

Extending Janus lectins architecture: Characterization and application to protocells

Autores: Simona Notova, Lina Siukstaite, Francesca Rosato, Federica Vena, Aymeric Audfray, Nicolai Bovin, Ludovic Landemarre, Winfried Römer, Anne Imberty
Publicado en: Computational and Structural Biotechnology Journal, Edición 20, 2022, Página(s) 6108-6119, ISSN 2001-0370
Editor: Elsevier
DOI: 10.1016/j.csbj.2022.11.005

Homo-and Heterovalent Neoglycoproteins as Ligands for Bacterial Lectins

Autores: David Goyard, Benoît Roubinet, Federica Vena, Ludovic Landemarre, Olivier Renaudet
Publicado en: ChemPlusChem, Edición 87/2, 2022, ISSN 2192-6506
Editor: Wiley-VCH Verlag
DOI: 10.1002/cplu.202100481

Microbial carbohydrate-binding toxins – From etiology to biotechnological application

Autores: Natalia Danielewicz; Francesca Rosato; Wenyue Dai; Winfried Römer; W. Bruce Turnbull; Juergen Mairhofer
Publicado en: Biotechnology Advances, Edición 59, 2022, ISSN 0734-9750
Editor: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107951

A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

Autores: Paras H. Kundalia , Lucia Pažitná, Kristína Kianičková, Eduard Jáné, Lenka Lorencová, Jaroslav Katrlík
Publicado en: Sensors, Edición 23(12), 2023, ISSN 1424-8220
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s23125362

Enzymatic Glyco-Modification of Synthetic Membrane Systems

Autores: Dylan Jabeguero, Lina Siukstaite, Chunyue Wang, Anna Mitrovic, Serge Pérez, Olga Makshakova, Ralf P. Richter, Winfried Römer and Christelle Breton
Publicado en: Biomolecules, Edición 13(2), 2023, Página(s) 335, ISSN 2218-273X
Editor: MDPI
DOI: 10.3390/biom13020335

Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy

Autores: Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Publicado en: ACS Omega, Edición 8 (17), 2023, Página(s) 15406–15421, ISSN 2470-1343
Editor: American Chemical Society
DOI: 10.1021/acsomega.3c00667

Targeted delivery of oligonucleotides using multivalent protein–carbohydrate interactions

Autores: Vajinder Kumar and W. Bruce Turnbull
Publicado en: Chemical Society Reviews, Edición 52, 2023, Página(s) 1273-1287, ISSN 1460-4744
Editor: Royal Society of Chemistry
DOI: 10.1039/d2cs00788f

Neutralizing the Impact of the Virulence Factor LecA from <i>Pseudomonas aeruginosa</i> on Human Cells with New Glycomimetic Inhibitors

Autores: Eva Zahorska; Francesca Rosato; Kai Stober; Sakonwan Kuhaudomlarp; Joscha Meiers; Dirk Hauck; Dorina Reith; Emilie Gillon; Katharina Rox; Anne Imberty; Winfried Römer; Alexander Titz
Publicado en: Angewandte Chemie International Edition, Edición 62, 2023, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202215535

The Lectin LecA Sensitizes the Human Stretch-Activated Channel TREK-1 but Not Piezo1 and Binds Selectively to Cardiac Non-myocytes

Autores: Elisa Darkow, Eva A. Rog-Zielinska, Josef Madl, Annette Brandel, Lina Siukstaite, Ramin Omidvar, Peter Kohl, Ursula Ravens, Winfried Römer, Rémi Peyronnet
Publicado en: Frontiers in Physiology, Edición 11, 2020, ISSN 1664-042X
Editor: Frontiers Research Foundation
DOI: 10.3389/fphys.2020.00457

In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli

Autores: Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Publicado en: Toxins, 2022, ISSN 2072-6651
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins14060396

MXene as a novel cartridge for N-glycan enrichment

Autores: Juvissan Aguedo, Zuzana Pakanova, Lenka Lorencova, Marek Nemcovic, Peter Kasak, Marek Barath, Pavol Farkas, JanTkac
Publicado en: Analytica Chimica Acta, Edición 00032670, 2022, Página(s) Volume 1234, ISSN 0003-2670
Editor: Elsevier BV
DOI: 10.1016/j.aca.2022.340512

A bispecific, crosslinking lectibody activates cytotoxic T cells and induces cancer cell death

Autores: Francesca Rosato, Rajeev Pasupuleti, Jana Tomisch, Ana Valeria Meléndez, Dajana Kolanovic, Olga N. Makshakova, Birgit Wiltschi & Winfried Römer
Publicado en: Journal of Translational Medicine, Edición 20/578, 2022, ISSN 1479-5876
Editor: BioMed Central
DOI: 10.1186/s12967-022-03794-w

Stabilization of membrane topologies by proteinaceous remorin scaffolds

Autores: Chao Su, Marta Rodriguez-Franco, Beatrice Lace, Nils Nebel, Casandra Hernandez-Reyes, Pengbo Liang, Eija Schulze, Evgeny V Mymrikov, Nikolas M Gross, Julian Knerr, Hong Wang, Lina Siukstaite, Jean Keller, Cyril Libourel, Alexandra AM Fischer, Katharina E Gabor, Eric Mark, Claudia Popp, Carola Hunte, Wilfried Weber, Petra Wendler, Thomas Stanislas, Pierre-Marc Delaux, Oliver Einsle, Robert Grosse,
Publicado en: Nature Comunications, Edición 14/323, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-35976-5

Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells

Autores: Ana Valeria Meléndez, Rubí M.-H. Velasco Cárdenas, Simon Lagies, Juliane Strietz, Lina Siukstaite, Oliver S. Thomas, Jana Tomisch, Wilfried Weber, Bernd Kammerer, Winfried Römer, Susana Minguet
Publicado en: Cellular and Molecular Life Sciences, Edición 1420682X, 2022, ISSN 1420-682X
Editor: Birkhauser Verlag
DOI: 10.1007/s00018-022-04524-7

Piggybacking on the Cholera Toxin: Identification of a CTB-Binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons.

Autores: Matthew R. Balmforth; Jessica L. Haigh; Vajinder Kumar; Vajinder Kumar; Wenyue Dai; Christian Tiede; Darren C. Tomlinson; Jim Deuchars; Michael E. Webb; W. Bruce Turnbull
Publicado en: Bioconjugate Chemistry, Edición 32 (10), 2021, Página(s) 2205–2212, ISSN 1043-1802
Editor: American Chemical Society
DOI: 10.1021/acs.bioconjchem.1c00373

The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3

Autores: Simona Notova; François Bonnardel; Francesca Rosato; Lina Siukstaite; Jessica Schwaiger; Jia Hui Lim; Nicolai Bovin; Annabelle Varrot; Yu Ogawa; Winfried Römer; Frédérique Lisacek; Anne Imberty
Publicado en: Communications Biology, Edición 5/954, 2022, ISSN 2399-3642
Editor: Nature Publising Group
DOI: 10.1038/s42003-022-03869-w

Polyvalent Transition-State Analogues of Sialyl Substrates Strongly Inhibit Bacterial Sialidases

Autores: Coralie Assailly, Clarisse Bridot, Dr. Amélie Saumonneau, Paul Lottin, Dr. Benoit Roubinet, Dr. Eva-Maria Krammer, Francesca François, Federica Vena, Dr. Ludovic Landemarre, Dr. Dimitri Alvarez Dorta, Dr. David Deniaud, Dr. Cyrille Grandjean, Dr. Charles Tellier, Dr. Sagrario Pascual, Dr. Véronique Montembault, Dr. Laurent Fontaine, Dr. Franck Daligault, Dr. Julie Bouckaert, Dr. Sébastien G. G
Publicado en: Chemistry – A European Journal, Edición 27, 2021, Página(s) 3142-3150, ISSN 1521-3765
Editor: Wiley-VCH
DOI: 10.1002/chem.202004672

Involvement of N‐glycans in binding of Photorhabdus luminescens Tc toxin

Autores: Peter Njenga Ng'ang'a, Lina Siukstaite, Alexander E. Lang, Hans Bakker, Winfried Römer, Klaus Aktories, Gudula Schmidt
Publicado en: Cellular Microbiology, Edición Monthly, 2021, ISSN 1462-5814
Editor: Blackwell Publishing Inc.
DOI: 10.1111/cmi.13326