Synthetic biology of carbohydrate-binding proteins: engineering protein-carbohydrate interactions for diagnostics and cell targeting

Informacje na temat projektu

synBIOcarb

Identyfikator umowy o grant: 814029

DOI

10.3030/814029

Projekt został zamknięty

Data podpisania przez KE 20 Sierpnia 2018

Data rozpoczęcia 1 Października 2018

Data zakończenia 31 Marca 2023

Finansowanie w ramach

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Koszt całkowity

€ 4 093 660,08

Wkład UE

€ 4 093 660,08

Koordynowany przez

UNIVERSITY OF LEEDS
United Kingdom

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Rezultaty

Other (1)

ESR project vacancies advertised

Websites, patent fillings, videos etc. (6)

E-newsletter 2 published

Enewsletter 2 published

International Conference on Synthetic Glycobiology
E-newsletter 1 published

Enewsletter 1 published

E-newsletter3 published

Enewsletter3 published

synBIOcarb website running (public section)

synBIOcarb website running public section

Report on one day contact meeting with industry

Publikacje

Other (2)

Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids

Autorzy: Sarah Wehrum; Daniel J Williamson; Lina Siukstaite; Thomas R Branson; Taras Sych; Josef Madl; Gemma C Wildsmith; Wenyue Dai; James F Ross; Maren Thomsen; Michael E Webb; Winfried Römer; W Bruce Turnbull
Opublikowane w: ChemRxiv, 2021
Wydawca: Cambridge University Press
DOI: 10.26434/chemrxiv-2021-j9stg

A pore-forming β-trefoil lectin with specificity for the tumor-related glycosphingolipid Gb3

Autorzy: Simona Notova, François Bonnardel, Francesca Rosato, Lina Siukstaite, Jessica Schwaiger, Nicolai Bovin, Annabelle Varrot, Winfried Römer, Frédérique Lisacek, Anne Imberty
Opublikowane w: BioRxiv, 2022
Wydawca: Cold Harbour Spring Laboratory
DOI: 10.1101/2022.02.10.479907

Peer reviewed articles (27)

Tuning specificity and topology of lectins through synthetic biology

Autorzy: Simona Notova, Anne Imberty
Opublikowane w: Current Opinion on Chemical Biology, Numer 73, 2023, Strona(/y) 102275, ISSN 1367-5931
Wydawca: Elsevier BV
DOI: 10.1016/j.cbpa.2023.102275

The Two Sweet Sides of Janus Lectin Drive Crosslinking of Liposomes to Cancer Cells and Material Uptake

Autorzy: Lina Siukstaite, Francesca Rosato, Anna Mitrovic, Peter Fritz Müller, Katharina Kraus, Simona Notova, Anne Imberty, Winfried Römer
Opublikowane w: Toxins, Numer 13/11, 2021, Strona(/y) 792, ISSN 2072-6651
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins13110792

Structural Diversities of Lectins Binding to the Glycosphingolipid Gb3

Autorzy: Lina Siukstaite, Anne Imberty, Winfried Römer
Opublikowane w: Frontiers in Molecular Biosciences, Numer 8, 2021, ISSN 2296-889X
Wydawca: Frontiers in Molecular Biosciences
DOI: 10.3389/fmolb.2021.704685

Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray

Autorzy: Tomas Berto, Aniko Bertokova, Eduard Jane, Michal Hires, Juvissan Aguedo, Maria Potocarova, Ludovit Lukac, Alica Vikartovska, Peter Kasak, Lubor Borsig and Jan Tkac
Opublikowane w: Frontiers in Oncology, Numer 11, 2021, Strona(/y) 735338, ISSN 2234-943X
Wydawca: Frontiers Media S. A.
DOI: 10.3389/fonc.2021.735338

Structure and engineering of tandem repeat lectins

Autorzy: Simona Notova, François Bonnardel, Frédérique Lisacek, Annabelle Varrot, Anne Imberty
Opublikowane w: Current Opinion in Structural Biology, Numer 62, 2020, Strona(/y) 39-47, ISSN 0959-440X
Wydawca: Elsevier BV
DOI: 10.1016/j.sbi.2019.11.006

Electrochemical Impedance Spectroscopy on 2D Nanomaterial MXene Modified Interfaces: Application as a Characterization and Transducing Tool

Autorzy: Juvissan Aguedo, Lenka Lorencova, Marek Barath, Pavol Farkas and Jan Tkac
Opublikowane w: Chemosensors, Numer 8/4, 2020, Strona(/y) 127, ISSN 2227-9040
Wydawca: MDPI
DOI: 10.3390/chemosensors8040127

Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids

Autorzy: Sarah Wehrum, Lina Siukstaite, Daniel J. Williamson, Thomas R. Branson, Taras Sych, Josef Madl, Gemma C. Wildsmith, Wenyue Dai, Erik Kempmann, James F. Ross, Maren Thomsen, Michael E. Webb, Winfried Römer, and W. Bruce Turnbull
Opublikowane w: ACS Synthetic Biology, Numer 11 (12), 2022, Strona(/y) 3929–3938, ISSN 2161-5063
Wydawca: American Chemical Society
DOI: 10.1021/acssynbio.2c00266

Genetic code expansion in E. coli enables production of a functional‘ready-to-click’ T cell receptor-specific scFv

Autorzy: Rajeev Pasupuleti, Francesca Rosato, Dajana Kolanovic, Olga N. Makshakova, Winfried Römer and Birgit Wiltschi
Opublikowane w: New Biotechnology, Numer 76, 2023, Strona(/y) 127-137, ISSN 1871-6784
Wydawca: Elsevier BV
DOI: 10.1016/j.nbt.2023.05.007

Building an Artificial Plant Cell Wall on a Lipid Bilayer by Assembling Polysaccharides and Engineered Proteins

Autorzy: Simona Notova; Nathan Cannac; Luca Rabagliati; Maeva Touzard; Josselin Mante; Yotam Navon; Liliane Coche-Guérente; Olivier Lerouxel; Laurent Heux; Anne Imberty
Opublikowane w: ACS Synthetic Biology, Numer 11/10, 2022, Strona(/y) 3516–3528, ISSN 2161-5063
Wydawca: American Chemical Society
DOI: 10.1021/acssynbio.2c00404

Extending Janus lectins architecture: Characterization and application to protocells

Autorzy: Simona Notova, Lina Siukstaite, Francesca Rosato, Federica Vena, Aymeric Audfray, Nicolai Bovin, Ludovic Landemarre, Winfried Römer, Anne Imberty
Opublikowane w: Computational and Structural Biotechnology Journal, Numer 20, 2022, Strona(/y) 6108-6119, ISSN 2001-0370
Wydawca: Elsevier
DOI: 10.1016/j.csbj.2022.11.005

Homo-and Heterovalent Neoglycoproteins as Ligands for Bacterial Lectins

Autorzy: David Goyard, Benoît Roubinet, Federica Vena, Ludovic Landemarre, Olivier Renaudet
Opublikowane w: ChemPlusChem, Numer 87/2, 2022, ISSN 2192-6506
Wydawca: Wiley-VCH Verlag
DOI: 10.1002/cplu.202100481

Microbial carbohydrate-binding toxins – From etiology to biotechnological application

Autorzy: Natalia Danielewicz; Francesca Rosato; Wenyue Dai; Winfried Römer; W. Bruce Turnbull; Juergen Mairhofer
Opublikowane w: Biotechnology Advances, Numer 59, 2022, ISSN 0734-9750
Wydawca: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107951

A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

Autorzy: Paras H. Kundalia , Lucia Pažitná, Kristína Kianičková, Eduard Jáné, Lenka Lorencová, Jaroslav Katrlík
Opublikowane w: Sensors, Numer 23(12), 2023, ISSN 1424-8220
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s23125362

Enzymatic Glyco-Modification of Synthetic Membrane Systems

Autorzy: Dylan Jabeguero, Lina Siukstaite, Chunyue Wang, Anna Mitrovic, Serge Pérez, Olga Makshakova, Ralf P. Richter, Winfried Römer and Christelle Breton
Opublikowane w: Biomolecules, Numer 13(2), 2023, Strona(/y) 335, ISSN 2218-273X
Wydawca: MDPI
DOI: 10.3390/biom13020335

Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy

Autorzy: Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Opublikowane w: ACS Omega, Numer 8 (17), 2023, Strona(/y) 15406–15421, ISSN 2470-1343
Wydawca: American Chemical Society
DOI: 10.1021/acsomega.3c00667

Targeted delivery of oligonucleotides using multivalent protein–carbohydrate interactions

Autorzy: Vajinder Kumar and W. Bruce Turnbull
Opublikowane w: Chemical Society Reviews, Numer 52, 2023, Strona(/y) 1273-1287, ISSN 1460-4744
Wydawca: Royal Society of Chemistry
DOI: 10.1039/d2cs00788f

Neutralizing the Impact of the Virulence Factor LecA from <i>Pseudomonas aeruginosa</i> on Human Cells with New Glycomimetic Inhibitors

Autorzy: Eva Zahorska; Francesca Rosato; Kai Stober; Sakonwan Kuhaudomlarp; Joscha Meiers; Dirk Hauck; Dorina Reith; Emilie Gillon; Katharina Rox; Anne Imberty; Winfried Römer; Alexander Titz
Opublikowane w: Angewandte Chemie International Edition, Numer 62, 2023, ISSN 1433-7851
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202215535

The Lectin LecA Sensitizes the Human Stretch-Activated Channel TREK-1 but Not Piezo1 and Binds Selectively to Cardiac Non-myocytes

Autorzy: Elisa Darkow, Eva A. Rog-Zielinska, Josef Madl, Annette Brandel, Lina Siukstaite, Ramin Omidvar, Peter Kohl, Ursula Ravens, Winfried Römer, Rémi Peyronnet
Opublikowane w: Frontiers in Physiology, Numer 11, 2020, ISSN 1664-042X
Wydawca: Frontiers Research Foundation
DOI: 10.3389/fphys.2020.00457

In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli

Autorzy: Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Opublikowane w: Toxins, 2022, ISSN 2072-6651
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins14060396

MXene as a novel cartridge for N-glycan enrichment

Autorzy: Juvissan Aguedo, Zuzana Pakanova, Lenka Lorencova, Marek Nemcovic, Peter Kasak, Marek Barath, Pavol Farkas, JanTkac
Opublikowane w: Analytica Chimica Acta, Numer 00032670, 2022, Strona(/y) Volume 1234, ISSN 0003-2670
Wydawca: Elsevier BV
DOI: 10.1016/j.aca.2022.340512

A bispecific, crosslinking lectibody activates cytotoxic T cells and induces cancer cell death

Autorzy: Francesca Rosato, Rajeev Pasupuleti, Jana Tomisch, Ana Valeria Meléndez, Dajana Kolanovic, Olga N. Makshakova, Birgit Wiltschi & Winfried Römer
Opublikowane w: Journal of Translational Medicine, Numer 20/578, 2022, ISSN 1479-5876
Wydawca: BioMed Central
DOI: 10.1186/s12967-022-03794-w

Stabilization of membrane topologies by proteinaceous remorin scaffolds

Autorzy: Chao Su, Marta Rodriguez-Franco, Beatrice Lace, Nils Nebel, Casandra Hernandez-Reyes, Pengbo Liang, Eija Schulze, Evgeny V Mymrikov, Nikolas M Gross, Julian Knerr, Hong Wang, Lina Siukstaite, Jean Keller, Cyril Libourel, Alexandra AM Fischer, Katharina E Gabor, Eric Mark, Claudia Popp, Carola Hunte, Wilfried Weber, Petra Wendler, Thomas Stanislas, Pierre-Marc Delaux, Oliver Einsle, Robert Grosse,
Opublikowane w: Nature Comunications, Numer 14/323, 2023, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-023-35976-5

Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells

Autorzy: Ana Valeria Meléndez, Rubí M.-H. Velasco Cárdenas, Simon Lagies, Juliane Strietz, Lina Siukstaite, Oliver S. Thomas, Jana Tomisch, Wilfried Weber, Bernd Kammerer, Winfried Römer, Susana Minguet
Opublikowane w: Cellular and Molecular Life Sciences, Numer 1420682X, 2022, ISSN 1420-682X
Wydawca: Birkhauser Verlag
DOI: 10.1007/s00018-022-04524-7

Piggybacking on the Cholera Toxin: Identification of a CTB-Binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons.

Autorzy: Matthew R. Balmforth; Jessica L. Haigh; Vajinder Kumar; Vajinder Kumar; Wenyue Dai; Christian Tiede; Darren C. Tomlinson; Jim Deuchars; Michael E. Webb; W. Bruce Turnbull
Opublikowane w: Bioconjugate Chemistry, Numer 32 (10), 2021, Strona(/y) 2205–2212, ISSN 1043-1802
Wydawca: American Chemical Society
DOI: 10.1021/acs.bioconjchem.1c00373

The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3

Autorzy: Simona Notova; François Bonnardel; Francesca Rosato; Lina Siukstaite; Jessica Schwaiger; Jia Hui Lim; Nicolai Bovin; Annabelle Varrot; Yu Ogawa; Winfried Römer; Frédérique Lisacek; Anne Imberty
Opublikowane w: Communications Biology, Numer 5/954, 2022, ISSN 2399-3642
Wydawca: Nature Publising Group
DOI: 10.1038/s42003-022-03869-w

Polyvalent Transition-State Analogues of Sialyl Substrates Strongly Inhibit Bacterial Sialidases

Autorzy: Coralie Assailly, Clarisse Bridot, Dr. Amélie Saumonneau, Paul Lottin, Dr. Benoit Roubinet, Dr. Eva-Maria Krammer, Francesca François, Federica Vena, Dr. Ludovic Landemarre, Dr. Dimitri Alvarez Dorta, Dr. David Deniaud, Dr. Cyrille Grandjean, Dr. Charles Tellier, Dr. Sagrario Pascual, Dr. Véronique Montembault, Dr. Laurent Fontaine, Dr. Franck Daligault, Dr. Julie Bouckaert, Dr. Sébastien G. G
Opublikowane w: Chemistry – A European Journal, Numer 27, 2021, Strona(/y) 3142-3150, ISSN 1521-3765
Wydawca: Wiley-VCH
DOI: 10.1002/chem.202004672

Involvement of N‐glycans in binding of Photorhabdus luminescens Tc toxin

Autorzy: Peter Njenga Ng'ang'a, Lina Siukstaite, Alexander E. Lang, Hans Bakker, Winfried Römer, Klaus Aktories, Gudula Schmidt
Opublikowane w: Cellular Microbiology, Numer Monthly, 2021, ISSN 1462-5814
Wydawca: Blackwell Publishing Inc.
DOI: 10.1111/cmi.13326