Synthetic biology of carbohydrate-binding proteins: engineering protein-carbohydrate interactions for diagnostics and cell targeting

Informazioni relative al progetto

synBIOcarb

ID dell’accordo di sovvenzione: 814029

DOI

10.3030/814029

Progetto chiuso

Data della firma CE 20 Agosto 2018

Data di avvio 1 Ottobre 2018

Data di completamento 31 Marzo 2023

Finanziato da

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Costo totale

€ 4 093 660,08

Contributo UE

€ 4 093 660,08

Coordinato da

UNIVERSITY OF LEEDS
United Kingdom

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Other (1)

ESR project vacancies advertised

Websites, patent fillings, videos etc. (6)

E-newsletter 2 published

Enewsletter 2 published

International Conference on Synthetic Glycobiology
E-newsletter 1 published

Enewsletter 1 published

E-newsletter3 published

Enewsletter3 published

synBIOcarb website running (public section)

synBIOcarb website running public section

Report on one day contact meeting with industry

Pubblicazioni

Other (2)

Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids

Autori: Sarah Wehrum; Daniel J Williamson; Lina Siukstaite; Thomas R Branson; Taras Sych; Josef Madl; Gemma C Wildsmith; Wenyue Dai; James F Ross; Maren Thomsen; Michael E Webb; Winfried Römer; W Bruce Turnbull
Pubblicato in: ChemRxiv, 2021
Editore: Cambridge University Press
DOI: 10.26434/chemrxiv-2021-j9stg

A pore-forming β-trefoil lectin with specificity for the tumor-related glycosphingolipid Gb3

Autori: Simona Notova, François Bonnardel, Francesca Rosato, Lina Siukstaite, Jessica Schwaiger, Nicolai Bovin, Annabelle Varrot, Winfried Römer, Frédérique Lisacek, Anne Imberty
Pubblicato in: BioRxiv, 2022
Editore: Cold Harbour Spring Laboratory
DOI: 10.1101/2022.02.10.479907

Peer reviewed articles (27)

Tuning specificity and topology of lectins through synthetic biology

Autori: Simona Notova, Anne Imberty
Pubblicato in: Current Opinion on Chemical Biology, Numero 73, 2023, Pagina/e 102275, ISSN 1367-5931
Editore: Elsevier BV
DOI: 10.1016/j.cbpa.2023.102275

The Two Sweet Sides of Janus Lectin Drive Crosslinking of Liposomes to Cancer Cells and Material Uptake

Autori: Lina Siukstaite, Francesca Rosato, Anna Mitrovic, Peter Fritz Müller, Katharina Kraus, Simona Notova, Anne Imberty, Winfried Römer
Pubblicato in: Toxins, Numero 13/11, 2021, Pagina/e 792, ISSN 2072-6651
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins13110792

Structural Diversities of Lectins Binding to the Glycosphingolipid Gb3

Autori: Lina Siukstaite, Anne Imberty, Winfried Römer
Pubblicato in: Frontiers in Molecular Biosciences, Numero 8, 2021, ISSN 2296-889X
Editore: Frontiers in Molecular Biosciences
DOI: 10.3389/fmolb.2021.704685

Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray

Autori: Tomas Berto, Aniko Bertokova, Eduard Jane, Michal Hires, Juvissan Aguedo, Maria Potocarova, Ludovit Lukac, Alica Vikartovska, Peter Kasak, Lubor Borsig and Jan Tkac
Pubblicato in: Frontiers in Oncology, Numero 11, 2021, Pagina/e 735338, ISSN 2234-943X
Editore: Frontiers Media S. A.
DOI: 10.3389/fonc.2021.735338

Structure and engineering of tandem repeat lectins

Autori: Simona Notova, François Bonnardel, Frédérique Lisacek, Annabelle Varrot, Anne Imberty
Pubblicato in: Current Opinion in Structural Biology, Numero 62, 2020, Pagina/e 39-47, ISSN 0959-440X
Editore: Elsevier BV
DOI: 10.1016/j.sbi.2019.11.006

Electrochemical Impedance Spectroscopy on 2D Nanomaterial MXene Modified Interfaces: Application as a Characterization and Transducing Tool

Autori: Juvissan Aguedo, Lenka Lorencova, Marek Barath, Pavol Farkas and Jan Tkac
Pubblicato in: Chemosensors, Numero 8/4, 2020, Pagina/e 127, ISSN 2227-9040
Editore: MDPI
DOI: 10.3390/chemosensors8040127

Membrane Fusion Mediated by Non-covalent Binding of Re-engineered Cholera Toxin Assemblies to Glycolipids

Autori: Sarah Wehrum, Lina Siukstaite, Daniel J. Williamson, Thomas R. Branson, Taras Sych, Josef Madl, Gemma C. Wildsmith, Wenyue Dai, Erik Kempmann, James F. Ross, Maren Thomsen, Michael E. Webb, Winfried Römer, and W. Bruce Turnbull
Pubblicato in: ACS Synthetic Biology, Numero 11 (12), 2022, Pagina/e 3929–3938, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.2c00266

Genetic code expansion in E. coli enables production of a functional‘ready-to-click’ T cell receptor-specific scFv

Autori: Rajeev Pasupuleti, Francesca Rosato, Dajana Kolanovic, Olga N. Makshakova, Winfried Römer and Birgit Wiltschi
Pubblicato in: New Biotechnology, Numero 76, 2023, Pagina/e 127-137, ISSN 1871-6784
Editore: Elsevier BV
DOI: 10.1016/j.nbt.2023.05.007

Building an Artificial Plant Cell Wall on a Lipid Bilayer by Assembling Polysaccharides and Engineered Proteins

Autori: Simona Notova; Nathan Cannac; Luca Rabagliati; Maeva Touzard; Josselin Mante; Yotam Navon; Liliane Coche-Guérente; Olivier Lerouxel; Laurent Heux; Anne Imberty
Pubblicato in: ACS Synthetic Biology, Numero 11/10, 2022, Pagina/e 3516–3528, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.2c00404

Extending Janus lectins architecture: Characterization and application to protocells

Autori: Simona Notova, Lina Siukstaite, Francesca Rosato, Federica Vena, Aymeric Audfray, Nicolai Bovin, Ludovic Landemarre, Winfried Römer, Anne Imberty
Pubblicato in: Computational and Structural Biotechnology Journal, Numero 20, 2022, Pagina/e 6108-6119, ISSN 2001-0370
Editore: Elsevier
DOI: 10.1016/j.csbj.2022.11.005

Homo-and Heterovalent Neoglycoproteins as Ligands for Bacterial Lectins

Autori: David Goyard, Benoît Roubinet, Federica Vena, Ludovic Landemarre, Olivier Renaudet
Pubblicato in: ChemPlusChem, Numero 87/2, 2022, ISSN 2192-6506
Editore: Wiley-VCH Verlag
DOI: 10.1002/cplu.202100481

Microbial carbohydrate-binding toxins – From etiology to biotechnological application

Autori: Natalia Danielewicz; Francesca Rosato; Wenyue Dai; Winfried Römer; W. Bruce Turnbull; Juergen Mairhofer
Pubblicato in: Biotechnology Advances, Numero 59, 2022, ISSN 0734-9750
Editore: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.107951

A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

Autori: Paras H. Kundalia , Lucia Pažitná, Kristína Kianičková, Eduard Jáné, Lenka Lorencová, Jaroslav Katrlík
Pubblicato in: Sensors, Numero 23(12), 2023, ISSN 1424-8220
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s23125362

Enzymatic Glyco-Modification of Synthetic Membrane Systems

Autori: Dylan Jabeguero, Lina Siukstaite, Chunyue Wang, Anna Mitrovic, Serge Pérez, Olga Makshakova, Ralf P. Richter, Winfried Römer and Christelle Breton
Pubblicato in: Biomolecules, Numero 13(2), 2023, Pagina/e 335, ISSN 2218-273X
Editore: MDPI
DOI: 10.3390/biom13020335

Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy

Autori: Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Pubblicato in: ACS Omega, Numero 8 (17), 2023, Pagina/e 15406–15421, ISSN 2470-1343
Editore: American Chemical Society
DOI: 10.1021/acsomega.3c00667

Targeted delivery of oligonucleotides using multivalent protein–carbohydrate interactions

Autori: Vajinder Kumar and W. Bruce Turnbull
Pubblicato in: Chemical Society Reviews, Numero 52, 2023, Pagina/e 1273-1287, ISSN 1460-4744
Editore: Royal Society of Chemistry
DOI: 10.1039/d2cs00788f

Neutralizing the Impact of the Virulence Factor LecA from <i>Pseudomonas aeruginosa</i> on Human Cells with New Glycomimetic Inhibitors

Autori: Eva Zahorska; Francesca Rosato; Kai Stober; Sakonwan Kuhaudomlarp; Joscha Meiers; Dirk Hauck; Dorina Reith; Emilie Gillon; Katharina Rox; Anne Imberty; Winfried Römer; Alexander Titz
Pubblicato in: Angewandte Chemie International Edition, Numero 62, 2023, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202215535

The Lectin LecA Sensitizes the Human Stretch-Activated Channel TREK-1 but Not Piezo1 and Binds Selectively to Cardiac Non-myocytes

Autori: Elisa Darkow, Eva A. Rog-Zielinska, Josef Madl, Annette Brandel, Lina Siukstaite, Ramin Omidvar, Peter Kohl, Ursula Ravens, Winfried Römer, Rémi Peyronnet
Pubblicato in: Frontiers in Physiology, Numero 11, 2020, ISSN 1664-042X
Editore: Frontiers Research Foundation
DOI: 10.3389/fphys.2020.00457

In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli

Autori: Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael E. Webb, Gerald Striedner,Winfried Römer,W. Bruce Turnbull, Juergen Mairhofer
Pubblicato in: Toxins, 2022, ISSN 2072-6651
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins14060396

MXene as a novel cartridge for N-glycan enrichment

Autori: Juvissan Aguedo, Zuzana Pakanova, Lenka Lorencova, Marek Nemcovic, Peter Kasak, Marek Barath, Pavol Farkas, JanTkac
Pubblicato in: Analytica Chimica Acta, Numero 00032670, 2022, Pagina/e Volume 1234, ISSN 0003-2670
Editore: Elsevier BV
DOI: 10.1016/j.aca.2022.340512

A bispecific, crosslinking lectibody activates cytotoxic T cells and induces cancer cell death

Autori: Francesca Rosato, Rajeev Pasupuleti, Jana Tomisch, Ana Valeria Meléndez, Dajana Kolanovic, Olga N. Makshakova, Birgit Wiltschi & Winfried Römer
Pubblicato in: Journal of Translational Medicine, Numero 20/578, 2022, ISSN 1479-5876
Editore: BioMed Central
DOI: 10.1186/s12967-022-03794-w

Stabilization of membrane topologies by proteinaceous remorin scaffolds

Autori: Chao Su, Marta Rodriguez-Franco, Beatrice Lace, Nils Nebel, Casandra Hernandez-Reyes, Pengbo Liang, Eija Schulze, Evgeny V Mymrikov, Nikolas M Gross, Julian Knerr, Hong Wang, Lina Siukstaite, Jean Keller, Cyril Libourel, Alexandra AM Fischer, Katharina E Gabor, Eric Mark, Claudia Popp, Carola Hunte, Wilfried Weber, Petra Wendler, Thomas Stanislas, Pierre-Marc Delaux, Oliver Einsle, Robert Grosse,
Pubblicato in: Nature Comunications, Numero 14/323, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-35976-5

Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells

Autori: Ana Valeria Meléndez, Rubí M.-H. Velasco Cárdenas, Simon Lagies, Juliane Strietz, Lina Siukstaite, Oliver S. Thomas, Jana Tomisch, Wilfried Weber, Bernd Kammerer, Winfried Römer, Susana Minguet
Pubblicato in: Cellular and Molecular Life Sciences, Numero 1420682X, 2022, ISSN 1420-682X
Editore: Birkhauser Verlag
DOI: 10.1007/s00018-022-04524-7

Piggybacking on the Cholera Toxin: Identification of a CTB-Binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons.

Autori: Matthew R. Balmforth; Jessica L. Haigh; Vajinder Kumar; Vajinder Kumar; Wenyue Dai; Christian Tiede; Darren C. Tomlinson; Jim Deuchars; Michael E. Webb; W. Bruce Turnbull
Pubblicato in: Bioconjugate Chemistry, Numero 32 (10), 2021, Pagina/e 2205–2212, ISSN 1043-1802
Editore: American Chemical Society
DOI: 10.1021/acs.bioconjchem.1c00373

The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3

Autori: Simona Notova; François Bonnardel; Francesca Rosato; Lina Siukstaite; Jessica Schwaiger; Jia Hui Lim; Nicolai Bovin; Annabelle Varrot; Yu Ogawa; Winfried Römer; Frédérique Lisacek; Anne Imberty
Pubblicato in: Communications Biology, Numero 5/954, 2022, ISSN 2399-3642
Editore: Nature Publising Group
DOI: 10.1038/s42003-022-03869-w

Polyvalent Transition-State Analogues of Sialyl Substrates Strongly Inhibit Bacterial Sialidases

Autori: Coralie Assailly, Clarisse Bridot, Dr. Amélie Saumonneau, Paul Lottin, Dr. Benoit Roubinet, Dr. Eva-Maria Krammer, Francesca François, Federica Vena, Dr. Ludovic Landemarre, Dr. Dimitri Alvarez Dorta, Dr. David Deniaud, Dr. Cyrille Grandjean, Dr. Charles Tellier, Dr. Sagrario Pascual, Dr. Véronique Montembault, Dr. Laurent Fontaine, Dr. Franck Daligault, Dr. Julie Bouckaert, Dr. Sébastien G. G
Pubblicato in: Chemistry – A European Journal, Numero 27, 2021, Pagina/e 3142-3150, ISSN 1521-3765
Editore: Wiley-VCH
DOI: 10.1002/chem.202004672

Involvement of N‐glycans in binding of Photorhabdus luminescens Tc toxin

Autori: Peter Njenga Ng'ang'a, Lina Siukstaite, Alexander E. Lang, Hans Bakker, Winfried Römer, Klaus Aktories, Gudula Schmidt
Pubblicato in: Cellular Microbiology, Numero Monthly, 2021, ISSN 1462-5814
Editore: Blackwell Publishing Inc.
DOI: 10.1111/cmi.13326