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Understanding host cellular systems that drive an endosymbiotic interaction

Descrizione del progetto

Meccanismi molecolari di endosimbiosi

L’endosimbiosi è il fenomeno di integrazione di una cellula all’interno di un’altra in modo da diventare un componente fondamentale del beneficiario, modificando le sue caratteristiche e creando una nuova traiettoria evoluzionista. Il Paramecium bursaria è un protista unicellulare con diverse alghe verdi al suo interno che formano un’endosimbiosi fototrofica. Tale rapporto è emergente e i partner possono essere separati e crescere separatamente con il successivo nuovo inizio di endosimbiosi. Il progetto CELL-in-CELL, finanziato dall’UE, per la prima volta individuerà le funzioni del gene responsabili dell’interazione endosimbiotica stabile. Per individuare i geni ospiti che stabiliscono l’endosimbiosi nel P. bursaria, i ricercatori hanno sviluppato una tecnologia dell’interferenza basata su RNA (RNAi) per il silenziamento. Essi condurranno il sequenziamento genomico, seguito da un esperimento di screening di knockdown tramite RNAi su larga scala per individuare i geni ospiti e le proteine coinvolte nel processo di endosimbiosi.

Obiettivo

Endosymbiosis is a key phenomenon that has played a critical role in shaping biological diversity, driving gene transfer and generating cellular complexity. During the process of endosymbiosis, one cell is integrated within another to become a critical component of the recipient, changing its characteristics and allowing it to chart a distinct evolutionary trajectory. Endosymbiosis was fundamentally important to the origin and evolution of eukaryotic cellular complexity, because an endosymbiotic event roots the diversification of all known eukaryotes and endosymbiosis has continually driven the diversification of huge sections of the eukaryotic tree of life. Little is known about how nascent endosymbioses are established or how they go on to form novel cellular compartments known as endosymbiotic organelles. Paramecium bursaria is a single celled protist that harbours multiple green algae within to form a phototrophic endosymbiosis. This relationship is nascent as the partners can be separated, grown separately, and the endosymbiosis reinitiated. This project will identify, for the first time, the gene functions that enable one cell to incubate another within to form a stable endosymbiotic interaction. To identify and explore which host genes control endosymbiosis in P. bursaria we have developed RNAi silencing technology. In the proposed project we will conduct genome sequencing, followed by a large-scale RNAi knockdown screening experiment, to identify host genes that when silenced perturb the endosymbiont population. Having identified candidate genes, we will investigate the localisation and function of the host encoded proteins. This project will significantly change our current understanding of the evolutionary phenomenon of endosymbiosis by identifying the cellular adaptations that drive these interactions, advancing our understanding of how these important moments in evolution occur and how core cellular systems can diversify in function.

Meccanismo di finanziamento

ERC-COG - Consolidator Grant

Istituzione ospitante

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
Contribution nette de l'UE
€ 2 397 385,76
Indirizzo
WELLINGTON SQUARE UNIVERSITY OFFICES
OX1 2JD Oxford
Regno Unito

Mostra sulla mappa

Regione
South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire
Tipo di attività
Higher or Secondary Education Establishments
Collegamenti
Costo totale
€ 2 397 385,76

Beneficiari (3)