Fostering Synthetic Biology standardisation through international collaboration

Risultati finali

Website and social media accounts The project website and the Social Media accounts will contain information on the aims partners work progress and current state of the project Month of Delivery M3 responsible partner DARWIN

Establishment of SB chassis of reference

Establishment of a limited number of SB chassis of reference Identification of target sequences genomewide analysis of relevant genetic determinants of the above mentioned species for specific performance scenarios and definition of streamlined genomes optimally adapted to each setting Accreditation of a minimum of 46 chassis extensible to about one dozen as hosts of engineered pathways for bioproduction or bioremediation The deliverable will make explicit the lowhanging fruits that can be reached out during the Project lifetime in terms of standardized platforms that can be established and which will become a benchmark for future additions to the collection Month of delivery M12 Responsible partner CSIC

Standards and ownership report

Standards and ownership report In this report we provide a detailed description of the activities carried out in WP5 and a discussion on responsibility in relation to knowledge infrastructures for synthetic biology Month of delivery M35 Responsible partner UiO

Review of standards in SB: detailing the gaps, challenges and opportunities

Review of standards in synthetic biology detailing the gaps challenges and opportunities Month of delivery M20 Responsible partner IMPERIAL

Online repository of existing and novel biocontainment strategies.

Online repository of existing and novel biocontainment strategies Month of Delivery M34 Responsible partner Biofaction

White paper of the BIOROBOOST project

White paper of the BIOROBOOST project The white paper will summarize all the advances conclusions and recommendations resulting from the project and will especially target the policy community Month of Delivery M34 responsible partner UVEG

White paper on exemplar metadata standards for use in biological metrology

D12 White paper on exemplar metadata standards and biological interdependencies for use in biological metrology Month of delivery M24 Responsible partner IMPERIAL EDIN ETH

Standardisation and multi-cellular systems report

Standardisation and multicellular systems report Month of delivery M34 responsible partner UEDINThe deliverables of this WP will include an overview of the modelling performed as well as recommendations

Standardisation and mammalian systems report

Standardisation and mammalian systems report Month of delivery M30 responsible partner UEDINThe deliverables of this WP will include an overview of the modelling performed as well as recommendations

Standardisation and yeast systems report

Standardisation and yeast systems report Month of delivery M28 responsible partner UEDIN The deliverables of this WP3 will include an overview of the modelling performed as well as recommendations

Report about incorporation efforts of safety relevant data and information in SB standards

Report about incorporation efforts of safety relevant data and information in SB standards Month of Delivery M24 Responsible partner Biofaction

A conceptual analysis of standards

A conceptual analysis of standards The first deliverable of WP4 is a description of an analytic framework for studying standards within the scope of the project The description will explain core theories how those together conceptualise standardisation and how the framework lends itself to the empirical research that will characterise WP4 and WP5 This document will serve all of those involved in these two work packages but it will also offer the rest of the project team a different perspective on the type of work that they are carrying out As such it plays both a functional role and a critical role Month of delivery M21 Responsible partner UEDIN

Workshop report about the initiation of the interface between science, industry and risk assessment authorities

Workshop report about the initiation of the interface between science industry and risk assessment authorities Month of Delivery Month of the first workshop plus 3 Months Responsible partner EMBO

A community map

A community map WP4 will also deliver a detailed description of relevant parties involved in the ongoing design and use of the standard The communities listed and described will form a representative set of people objects institutions and practices The map will describe key relationships between the different communities The map will be constructed using methods commonly employed in the social sciences Specifically the projects participants will serve as the first actors identified and then by investigating their connections the map will be expanded in steps Short qualitative interviews may be used to acquire greater detail about relations when necessaryThe map will serve several ends First overviews of social groups and their relationships are crucial to social scientific research It will support the rest of work carried out in WP4 which focuses on the social infrastructure that supports standards including relationships between people and their institutions Second it will contribute to WP5 and help link both packages We place value in working together in order to sustain an extended social scientific perspective Third it will function as a basis against with which to track changes in the network something which has both social scientific benefits and can help scientific practitioners to comprehend how their community is developing and what vital parties may be missing This deliverable will be made possible by the empirical data gathered which will also be taken up and expanded in WP5 Month of delivery M24 Responsible partner UEDIN

Upgraded SEVA platform

Upgraded SEVA platform with genetic tools for the set of chassis adopted in BIOROBOOST and made accessible to the community through an automated on line requestretrievaldelivery system operating on an Open Source basis Month of delivery M12 Responsible partner CSIC

Tools for modelling chassis-implant and chassis-niche interplay

Tools for modelling chassisimplant and chassisniche interplay Implementation of complete genomic and metabolic models of each of the chassis and the interactions with implants and with selected environmental parameters specific of each application setting SBML will be used to record such parameter sets And also the extension of SBOL to include the description of chassis bacterial compartments that is not possible with current SBOL version Month of delivery M12 Responsible partner UNEW

Pubblicazioni

Concomitant prediction of environmental fate and toxicity of chemical compounds

Autori: Juan Antonio Garcia-Martin, Max Chavarría, Victor de Lorenzo, Florencio Pazos
Pubblicato in: Biology Methods and Protocols, Numero 5/1, 2020, ISSN 2396-8923
Editore: Oxford University Press
DOI: 10.1093/biomethods/bpaa025

Targeted Depletion of Bacteria from Mixed Populations by Programmable Adhesion with Antagonistic Competitor Cells

Autori: See-Yeun Ting, Esteban Martínez-García, Shuo Huang, Savannah K. Bertolli, Katherine A. Kelly, Kevin J. Cutler, Elizabeth D. Su, Hui Zhi, Qing Tang, Matthew C. Radey, Manuela Raffatellu, S. Brook Peterson, Víctor de Lorenzo, Joseph D. Mougous
Pubblicato in: Cell Host & Microbe, Numero 28/2, 2020, Pagina/e 313-321.e6, ISSN 1931-3128
Editore: Cell Press
DOI: 10.1016/j.chom.2020.05.006

Out of the Abyss: Genome and Metagenome Mining Reveals Unexpected Environmental Distribution of Abyssomicins

Autori: Alba Iglesias, Adriel Latorre-Pérez, James E. M. Stach, Manuel Porcar, Javier Pascual
Pubblicato in: Frontiers in Microbiology, Numero 11, 2020, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2020.00645

The long journey towards standards for engineering biosystems

Autori: Jacob Beal, Angel Goñi‐Moreno, Chris Myers, Ariel Hecht, María del Carmen Vicente, Maria Parco, Markus Schmidt, Kenneth Timmis, Geoff Baldwin, Steffi Friedrichs, Paul Freemont, Daisuke Kiga, Elena Ordozgoiti, Maja Rennig, Leonardo Rios, Kristie Tanner, Víctor Lorenzo, Manuel Porcar
Pubblicato in: EMBO reports, Numero 21/5, 2020, ISSN 1469-221X
Editore: Nature Publishing Group
DOI: 10.15252/embr.202050521

Predictive design of sigma factor-specific promoters

Autori: Maarten Van Brempt, Jim Clauwaert, Friederike Mey, Michiel Stock, Jo Maertens, Willem Waegeman, Marjan De Mey
Pubblicato in: Nature Communications, Numero 11/1, 2020, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-020-19446-w

A Bifan Motif Shaped by ArsR1, ArsR2, and Their Cognate Promoters Frames Arsenic Tolerance of Pseudomonas putida

Autori: Gonzalo Durante-Rodríguez, David Páez-Espino, Víctor de Lorenzo
Pubblicato in: Frontiers in Microbiology, Numero 12, 2021, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2021.641440

Exploiting geometric similarity for statistical quantification of fluorescence spatial patterns in bacterial colonies

Autori: David R. Espeso, Elena Algar, Esteban Martínez-García, Víctor de Lorenzo
Pubblicato in: BMC Bioinformatics, Numero 21/1, 2020, ISSN 1471-2105
Editore: BioMed Central
DOI: 10.1186/s12859-020-3490-1

The long and winding road: Accidents and tinkering in software standardization

Autori: Sergi Valverde
Pubblicato in: Mètode Revista de difusió de la investigació, Numero 11, 2020, ISSN 2174-9221
Editore: University of Valencia
DOI: 10.7203/metode.11.16112

ArsH protects Pseudomonas putida from oxidative damage caused by exposure to arsenic

Autori: A. David Páez‐Espino, Pablo I. Nikel, Max Chavarría, Víctor Lorenzo
Pubblicato in: Environmental Microbiology, Numero 22/6, 2020, Pagina/e 2230-2242, ISSN 1462-2912
Editore: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14991

Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2

Autori: Juhyun Kim, Angel Goñi-Moreno, Víctor de Lorenzo
Pubblicato in: mBio, Numero 12/1, 2021, ISSN 2150-7511
Editore: ASM Journals
DOI: 10.1128/mbio.03685-20

Biotransformation of d ‐xylose to d ‐xylonate coupled to medium‐chain‐length polyhydroxyalkanoate production in cellobiose‐grown Pseudomonas putida EM42

Autori: Pavel Dvořák, Jozef Kováč, Víctor Lorenzo
Pubblicato in: Microbial Biotechnology, Numero 13/4, 2020, Pagina/e 1273-1283, ISSN 1751-7915
Editore: Wiley_Society for applied microbiology
DOI: 10.1111/1751-7915.13574

The wasted chewing gum bacteriome

Autori: Leila Satari, Alba Guillén, Àngela Vidal-Verdú, Manuel Porcar
Pubblicato in: Scientific Reports, Numero 10/1, 2020, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-020-73913-4

Linking Engineered Cells to Their Digital Twins: A Version Control System for Strain Engineering

Autori: Jonathan Tellechea-Luzardo, Charles Winterhalter, Paweł Widera, Jerzy Kozyra, Víctor de Lorenzo, Natalio Krasnogor
Pubblicato in: ACS Synthetic Biology, Numero 9/3, 2020, Pagina/e 536-545, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.9b00400

Engineering and standardization of posttranscriptional biocircuitry in Saccharomyces cerevisiae

Autori: John McCarthy
Pubblicato in: Integrative Biology, Numero 13/8, 2021, Pagina/e 210-220, ISSN 1757-9708
Editore: Oxford University Press
DOI: 10.1093/intbio/zyab013

High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

Autori: Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo
Pubblicato in: iScience, Numero 23/3, 2020, Pagina/e 100946, ISSN 2589-0042
Editore: Elsevier
DOI: 10.1016/j.isci.2020.100946

Chemically Stressed Bacterial Communities in Anaerobic Digesters Exhibit Resilience and Ecological Flexibility

Autori: Benjamin Schwan, Christian Abendroth, Adriel Latorre-Pérez, Manuel Porcar, Cristina Vilanova, Christina Dornack
Pubblicato in: Frontiers in Microbiology, Numero 11, 2020, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2020.00867

An automated DIY framework for experimental evolution of Pseudomonas putida

Autori: David R. Espeso, Pavel Dvořák, Tomás Aparicio, Víctor Lorenzo
Pubblicato in: Microbial Biotechnology, 2020, ISSN 1751-7915
Editore: John Wiley & Sons
DOI: 10.1111/1751-7915.13678

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Autori: Ángeles Hueso‐Gil, Belén Calles, Víctor Lorenzo
Pubblicato in: Environmental Microbiology, Numero 22/8, 2020, Pagina/e 3535-3547, ISSN 1462-2912
Editore: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15126

Robust estimation of bacterial cell count from optical density

Autori: Jacob Beal, Natalie G. Farny, Traci Haddock-Angelli, Vinoo Selvarajah, Geoff S. Baldwin, Russell Buckley-Taylor, Markus Gershater, Daisuke Kiga, John Marken, Vishal Sanchania, Abigail Sison, Christopher T. Workman
Pubblicato in: Communications Biology, Numero 3/1, 2020, ISSN 2399-3642
Editore: Springer Nature Limited
DOI: 10.1038/s42003-020-01127-5

Environmental Performance of Pseudomonas putida with a Uracylated Genome

Autori: Elena Algar, Yamal Al‐Ramahi, Víctor Lorenzo, Esteban Martínez‐García
Pubblicato in: ChemBioChem, Numero 21/22, 2020, Pagina/e 3255-3265, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000330

Y la biología se convirtió en ingeniería: La adopción de estándares para sistemas vivos

Autori: Victor De Lorenzo
Pubblicato in: Mètode Revista de difusió de la investigació, Numero 11, 2020, Pagina/e 67-73, ISSN 2174-9221
Editore: University of Valencia
DOI: 10.7203/metode.11.15975

Draft Genome Sequence of a New Oscillospiraceae Bacterium Isolated from Anaerobic Digestion of Biomass

Autori: Javier Pascual, Sarah Hahnke, Christian Abendroth, Thomas Langer, Patrice Ramm, Michael Klocke, Olaf Luschnig, Manuel Porcar
Pubblicato in: Microbiology Resource Announcements, Numero 9/27, 2020, ISSN 2576-098X
Editore: AMS Journals
DOI: 10.1128/mra.00507-20

Towards the right standards: The intersection of open science, responsible research and innovation, and standards

Autori: Michele S. Garfinkel
Pubblicato in: Mètode Revista de difusió de la investigació, Numero 11, 2020, Pagina/e 99-103, ISSN 2174-9221
Editore: University of Valencia
DOI: 10.7203/metode.11.16103

Can life be standardized? Current challenges in biological standardization

Autori: Juli Peretó, Manuel Porcar
Pubblicato in: Mètode Revista de difusió de la investigació, Numero 11, 2020, ISSN 2174-9221
Editore: University of Valencia
DOI: 10.7203/metode.11.15981

Low CyaA expression and anti‐cooperative binding of cAMP to CRP frames the scope of the cognate regulon of Pseudomonas putida

Autori: Alejandro Arce‐Rodríguez, Pablo I. Nikel, Belén Calles, Max Chavarría, Raúl Platero, Tino Krell, Victor Lorenzo
Pubblicato in: Environmental Microbiology, Numero 23/3, 2021, Pagina/e 1732-1749, ISSN 1462-2912
Editore: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15422

For the sake of the Bioeconomy: define what a Synthetic Biology Chassis is!

Autori: Víctor de Lorenzo, Natalio Krasnogor, Markus Schmidt
Pubblicato in: New Biotechnology, Numero 60, 2021, Pagina/e 44-51, ISSN 1871-6784
Editore: Elsevier BV
DOI: 10.1016/j.nbt.2020.08.004

How To Quantify a Genetic Firewall? A Polarity‐Based Metric for Genetic Code Engineering

Autori: Markus Schmidt, Vladimir Kubyshkin
Pubblicato in: ChemBioChem, 2020, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000758

Vida fabricada

Autori: Manuel Porcar, Juli Peretó
Pubblicato in: Mètode Revista de difusió de la investigació, Numero 10, 2019, ISSN 2174-9221
Editore: Mètode Sciences Journal- University of Valencia
DOI: 10.7203/metode.10.13229

Words, images and gender

Autori: Manuel Porcar, Adriel Latorre‐Pérez, Esther Molina‐Menor, Martí Domínguez
Pubblicato in: EMBO reports, Numero 20/7, 2019, ISSN 1469-221X
Editore: Nature Publishing Group
DOI: 10.15252/embr.201948401

Microbial communities of the Mediterranean rocky shore: ecology and biotechnological potential of the sea‐land transition

Autori: Esther Molina‐Menor, Kristie Tanner, Àngela Vidal‐Verdú, Juli Peretó, Manuel Porcar
Pubblicato in: Microbial Biotechnology, Numero September 2019, 2019, ISSN 1751-7915
Editore: Wiley
DOI: 10.1111/1751-7915.13475

Pathways to cellular supremacy in biocomputing

Autori: Lewis Grozinger, Martyn Amos, Thomas E. Gorochowski, Pablo Carbonell, Diego A. Oyarzún, Ruud Stoof, Harold Fellermann, Paolo Zuliani, Huseyin Tas, Angel Goñi-Moreno
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-13232-z

The rose and the name: the unresolved debate on biotechnological terms

Autori: Martí Domínguez, Juli Peretó, Manuel Porcar
Pubblicato in: Microbial Biotechnology, 2019, ISSN 1751-7915
Editore: society for applied microbiology
DOI: 10.1111/1751-7915.13522

Creating life and the media: translations and echoes

Autori: Manuel Porcar, Juli Peretó
Pubblicato in: Life Sciences, Society and Policy, Numero 14/1, 2018, ISSN 2195-7819
Editore: BMC - Springer Nature
DOI: 10.1186/s40504-018-0087-9

Complete Genome Sequence of a New Bacteroidaceae Bacterium Isolated from Anaerobic Biomass Digestion

Autori: Sarah Hahnke, Christian Abendroth, Javier Pascual, Thomas Langer, Francisco M. Codoñer, Patrice Ramm, Michael Klocke, Olaf Luschnig, Manuel Porcar
Pubblicato in: Microbiology Resource Announcements, Numero 8/46, 2019, ISSN 2576-098X
Editore: American Society for Microbiology
DOI: 10.1128/mra.01203-19

Synthetic microbiology as a source of new enterprises and job creation: a Mediterranean perspective

Autori: Cristina Vilanova, Manuel Porcar
Pubblicato in: Microbial Biotechnology, Numero 12/1, 2019, Pagina/e 8-10, ISSN 1751-7915
Editore: Society for Applied Microbiology
DOI: 10.1111/1751-7915.13326

The Hidden Charm of Life

Autori: Manuel Porcar
Pubblicato in: Life, Numero 9/1, 2019, Pagina/e 5, ISSN 2075-1729
Editore: MDPI
DOI: 10.3390/life9010005

ShortBOL: A Language for Scripting Designs for Engineered Biological Systems Using Synthetic Biology Open Language (SBOL)

Autori: Matthew Crowther, Lewis Grozinger, Matthew Pocock, Christopher P. D. Taylor, James A. McLaughlin, Göksel Mısırlı, Bryan A. Bartley, Jacob Beal, Angel Goñi-Moreno, Anil Wipat
Pubblicato in: ACS Synthetic Biology, 2020, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.9b00470

Modulating transcription through development of semi-synthetic yeast core promoters

Autori: Thomas Decoene, Sofie L. De Maeseneire, Marjan De Mey
Pubblicato in: PLOS ONE, Numero 14/11, 2019, Pagina/e e0224476, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0224476

A Model for the Spatiotemporal Design of Gene Regulatory Circuits

Autori: Ruud Stoof, Alexander Wood, Ángel Goñi-Moreno
Pubblicato in: ACS Synthetic Biology, Numero 8/9, 2019, Pagina/e 2007-2016, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.9b00022

Dynamical Task Switching in Cellular Computers

Autori: Angel Goñi-Moreno, Fernando de la Cruz, Alfonso Rodríguez-Patón, Martyn Amos
Pubblicato in: Life, Numero 9/1, 2019, Pagina/e 14, ISSN 2075-1729
Editore: MDPI
DOI: 10.3390/life9010014

SBOL-OWL: An Ontological Approach for Formal and Semantic Representation of Synthetic Biology Information

Autori: Göksel Mısırlı, Renee Taylor, Angel Goñi-Moreno, James Alastair McLaughlin, Chris Myers, John H. Gennari, Phillip Lord, Anil Wipat
Pubblicato in: ACS Synthetic Biology, Numero 8/7, 2019, Pagina/e 1498-1514, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.8b00532

Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1

Autori: Martina Carrillo, Marcel Wagner, Florian Petit, Amelie Dransfeld, Anke Becker, Tobias J. Erb
Pubblicato in: ACS Synthetic Biology, Numero 8/11, 2019, Pagina/e 2451-2456, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.9b00220

Sphingomonas solaris sp. nov., isolated from a solar panel in Boston, Massachusetts

Autori: Kristie Tanner, Christopher P. Mancuso, Juli Peretó, Ahmad S. Khalil, Cristina Vilanova, Javier Pascual
Pubblicato in: International Journal of Systematic and Evolutionary Microbiology, Numero 70/3, 2020, Pagina/e 1814-1821, ISSN 1466-5026
Editore: Society for General Microbiology
DOI: 10.1099/ijsem.0.003977

SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts

Autori: Esteban Martínez-García, Angel Goñi-Moreno, Bryan Bartley, James McLaughlin, Lucas Sánchez-Sampedro, Héctor Pascual del Pozo, Clara Prieto Hernández, Ada Serena Marletta, Davide De Lucrezia, Guzmán Sánchez-Fernández, Sofía Fraile, Víctor de Lorenzo
Pubblicato in: Nucleic Acids Research, Numero 48/D1, 2019, Pagina/e D1164-D1170, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkz1024

Multiple-site diversification of regulatory sequences enables inter-species operability of genetic devices

Autori: Hueso-Gil, Ángeles, Nyerges, Ákos, Pál, Csaba, Calles, Belén, de Lorenzo, Víctor
Pubblicato in: ACS Synthetic Biology, Numero December 3, 2019, 2019, Pagina/e 104–114, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.9b00375

Digitalizing heterologous gene expression in Gram‐negative bacteria with a portable ON/OFF module

Autori: Belén Calles, Ángel Goñi‐Moreno, Víctor Lorenzo
Pubblicato in: Molecular Systems Biology, Numero 15/12, 2019, ISSN 1744-4292
Editore: Nature Publishing Group
DOI: 10.15252/msb.20188777

Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene

Autori: Tomas Aparicio, Akos Nyerges, István Nagy, Csaba Pal, Esteban Martínez‐García, Víctor Lorenzo
Pubblicato in: Environmental Microbiology, Numero 22/1, 2019, Pagina/e 45-58, ISSN 1462-2912
Editore: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14814

Recombination independent genome editing through CRISPR/Cas9-enhanced targetron delivery

Autori: Elena Velázquez, Víctor de Lorenzo,Yamal Al-Ramahi
Pubblicato in: ACS Synthetic Biology, Numero August 16, 2019, 2019, Pagina/e 2186–2193, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.9b00293

Functional implementation of a linear glycolysis for sugar catabolism in Pseudomonas putida

Autori: Alberto Sánchez-Pascuala, Lorena Fernández-Cabezón, Víctor de Lorenzo, Pablo I. Nikel
Pubblicato in: Metabolic Engineering, Numero 54, 2019, Pagina/e 200-211, ISSN 1096-7176
Editore: Academic Press
DOI: 10.1016/j.ymben.2019.04.005

Pseudomonas putida in the quest of programmable chemistry

Autori: Esteban Martínez-García, Víctor de Lorenzo
Pubblicato in: Current Opinion in Biotechnology, Numero 59, 2019, Pagina/e 111-121, ISSN 0958-1669
Editore: Elsevier BV
DOI: 10.1016/j.copbio.2019.03.012

Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies

Autori: Sofía Fraile, María Briones, Mónica Revenga-Parra, Víctor de Lorenzo, Encarnación Lorenzo, Esteban Martínez-García
Pubblicato in: ACS Synthetic Biology, Numero 10/8, 2021, Pagina/e 2049-2059, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.1c00227

Standardisation and social ordering: A change of perspective

Autori: Pablo Schyfter
Pubblicato in: Mètode Revista de difusió de la investigació, Numero 11, 2020, ISSN 2174-9221
Editore: University of Valencia
DOI: 10.7203/metode.11.16013

Extremophilic microbial communities on photovoltaic panel surfaces: a two‐year study

Autori: Kristie Tanner, Esther Molina‐Menor, Adriel Latorre‐Pérez, Àngela Vidal‐Verdú, Cristina Vilanova, Juli Peretó, Manuel Porcar
Pubblicato in: Microbial Biotechnology, Numero 13/6, 2020, Pagina/e 1819-1830, ISSN 1751-7915
Editore: John Wiley & Sons
DOI: 10.1111/1751-7915.13620

The faulty SOS response of Pseudomonas putida KT2440 stems from an inefficient RecA‐LexA interplay

Autori: Özlem Akkaya, Tomás Aparicio, Danilo Pérez‐Pantoja, Víctor Lorenzo
Pubblicato in: Environmental Microbiology, Numero 23/3, 2021, Pagina/e 1608-1619, ISSN 1462-2912
Editore: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15384

Contextual dependencies expand the re-usability of genetic inverters

Autori: Huseyin Tas, Lewis Grozinger, Ruud Stoof, Victor de Lorenzo, Ángel Goñi-Moreno
Pubblicato in: Nature Communications, Numero 12/1, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-020-20656-5

DNA-BOT: a low-cost, automated DNA assembly platform for synthetic biology

Autori: Marko Storch, Matthew C Haines, Geoff S Baldwin
Pubblicato in: Synthetic Biology, Numero 5/1, 2020, ISSN 2397-7000
Editore: Oxford University Press
DOI: 10.1093/synbio/ysaa010

An updated structural model of the A domain of the Pseudomonas putida XylR regulator poses an atypical interplay with aromatic effectors

Autori: Pavel Dvořák, Carlos Alvarez‐Carreño, Sergio Ciordia, Alberto Paradela, Víctor Lorenzo
Pubblicato in: Environmental Microbiology, Numero 23/8, 2021, Pagina/e 4418-4433, ISSN 1462-2912
Editore: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15628

A SsrA/NIa-based Strategy for Post-Translational Regulation of Protein Levels in Gram-negative Bacteria

Autori: Gonzalo Durante-Rodríguez, Belén Calles, Víctor Lorenzo, Pablo Nikel
Pubblicato in: BIO-PROTOCOL, Numero 10/14, 2020, ISSN 2331-8325
Editore: Bio-protocol
DOI: 10.21769/bioprotoc.3688

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

Autori: Pablo I. Nikel, Tobias Fuhrer, Max Chavarría, Alberto Sánchez-Pascuala, Uwe Sauer, Víctor de Lorenzo
Pubblicato in: The ISME Journal, Numero 15/6, 2021, Pagina/e 1751-1766, ISSN 1751-7362
Editore: Nature Publishing Group
DOI: 10.1038/s41396-020-00884-9

Future‐proofing synthetic biology: educating the next generation

Autori: Jennifer S. Hallinan, Anil Wipat, Richard Kitney, Simon Woods, Ken Taylor, Angel Goñi‐Moreno
Pubblicato in: Engineering Biology, Numero 3/2, 2019, Pagina/e 25-31, ISSN 2398-6182
Editore: John Wiley & Sons
DOI: 10.1049/enb.2019.0001

A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria

Autori: Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
Pubblicato in: Horizontal Gene Transfer - Methods and Protocols, Numero 2075, 2020, Pagina/e 383-398, ISBN 978-1-4939-9876-0
Editore: Springer US
DOI: 10.1007/978-1-4939-9877-7_27

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