Project description
Insight into the kinetics of RNA life cycle
Conventional RNA sequencing methods provide an indication of the qualitative and quantitative changes in all RNA species within a cell. This means that it is not possible to discriminate between newly synthesised and existing RNA species in a time-resolved manner. The EU-funded SLAMseq project will focus on the recently developed thiol-linked alkylation for the metabolic sequencing of RNA (SLAMseq) method that allows the quantification of newly synthesised RNA. Apart from insight into the kinetics of gene expression, researchers will employ SLAMseq to determine the biogenesis and turnover of various RNA molecules, such as microRNAs.
Objective
Current gene expression profiling by massive parallel RNA sequencing provides insight into relative abundance of RNA molecules but fails to resolve the underlying principles in gene regulation that are reflected in the intracellular kinetics of RNA biogenesis, processing and turnover. In the ERC-StG 'miRLIFE', we have developed a novel technology – SLAMseq – that uncovers a chemically modified nucleoside analog by sequencing. When coupled to metabolic RNA labeling and targeted RNA sequencing, SLAMseq provides unprecedented insights into the intracellular kinetics of gene expression in a rapid, scalable and cost-effective manner. Preliminary data show that the SLAMseq technology can be used to modify other nucleoside analogs and be applied for non-targeted RNA seq protocols. In this PoC, we aim to validate and prove this broader applicability of SLAMseq in order to enable and commercialize genome-wide, time-resolved transcriptomics. In this way, metabolic RNA sequencing will revolutionise biomedical research by providing experimental access to the molecular basis of human malignancies and treatments thereof.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
ERC-POC - Proof of Concept Grant
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2018-PoC
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1030 WIEN
Austria
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.