Induced pluripotent stem cell-based therapy for spinal regeneration

Información del proyecto

iPSpine

Identificador del acuerdo de subvención: 825925

DOI

10.3030/825925

Proyecto cerrado

Fecha de la firma de la CE 7 Diciembre 2018

Fecha de inicio 1 Enero 2019

Fecha de finalización 30 Junio 2024

Financiado con arreglo a

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Coste total

€ 15 396 031,25

Aportación de la UE

€ 14 959 781,25

Coordinado por

UNIVERSITEIT UTRECHT
Netherlands

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (4)

Updated Dissemination and Exploitation plan
Set up of Patient Advisory Board
Guidelines and requirements for pre-in vivo evaluation of IVD advanced therapeutics

Guidelines and requirements for prein vivo evaluation of IVD advanced therapeutics

Report to the PSC on Dissemination and Education activities

Open Research Data Pilot (1)

Data Management Plan

Other (1)

Optimized open-access knowledge platform available

Websites, patent fillings, videos etc. (2)

Project newsletter
Project website

Publicaciones

Peer reviewed articles (38)

Spheroid-Like Cultures for Expanding Angiopoietin Receptor-1 (aka. Tie2) Positive Cells from the Human Intervertebral Disc

Autores: Xingshuo Zhang, Julien Guerrero, Andreas S. Croft, Christoph E. Albers, Sonja Häckel, Benjamin Gantenbein
Publicado en: International Journal of Molecular Sciences, Edición 21/24, 2020, Página(s) 9423, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21249423

Roles and responsibilities in stem cell research: a focus group study with stem cell researchers and patients

Autores: Lars S Assen, Karin R Jongsma, Rosario Isasi, Marianna A Tryfonidou & Annelien L Bredenoord
Publicado en: Regenerative Medicine, 2022, ISSN 2613-5914
Editor: Future Medicine
DOI: 10.2217/rme-2022-0019

Harmonization and standardization of nucleus pulposus cell extraction and culture methods

Autores: Shaghayegh Basatvat, Frances C. Bach, Marcos N. Barcellona, Abbie L. Binch, Conor T. Buckley, Brian Bueno, Nadeen O. Chahine, Ana Chee, Laura B. Creemers, Stefan Dudli, Bailey Fearing, Stephen J. Ferguson, Jennifer Gansau, Benjamin Gantenbein, Rahul Gawri, Juliane D. Glaeser, Sibylle Grad, Julien Guerrero, Lisbet Haglund, Paula A. Hernandez, Judith A. Hoyland, Charles Huang, James C. Iatridis, Sve
Publicado en: JOR Spine, 2023, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1238

Detergent-Free Decellularization of Notochordal Cell-Derived Matrix Yields a Regenerative, Injectable, and Swellable Biomaterial

Autores: Tara C. Schmitz; Marina van Doeselaar; Marianna A. Tryfonidou; Keita Ito
Publicado en: Tissue Engineering and Regenerative Medicine, Edición 10, 2022, ISSN 1932-7005
Editor: ACS Publications
DOI: 10.1021/acsbiomaterials.2c00790

Cell sources proposed for nucleus pulposus regeneration

Autores: Williams, Rebecca J.; Tryfonidou, Marianna A.; Snuggs, Joseph William; Le Maitre, Christine Lyn
Publicado en: JOR Spine, 2021, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1175

Notochordal Cell-Based Treatment Strategies and Their Potential in Intervertebral Disc Regeneration

Autores: Frances C Bach 1, Deepani W Poramba-Liyanage 1, Frank M Riemers 1, Jerome Guicheux 2 3 4, Anne Camus 2, James C Iatridis 5, Danny Chan 6, Keita Ito 7 8, Christine L Le Maitre 9, Marianna A Tryfonidou 1
Publicado en: Front Cell Dev Biol, 2021, ISSN 2296-634X
Editor: Frontiers
DOI: 10.3389/fcell.2021.780749

The nucleus pulposus microenvironment in the intervertebral disc: the fountain of youth?

Autores: Guerrero, J.; Häckel, S.; Croft, A.S.; Hoppe, S.; Albers, C.E.; Gantenbein, B.
Publicado en: eCM (Eur Cell Mater / e Cells & Materials), Edición 41, 2021, Página(s) 707-738, ISSN 1473-2262
Editor: Swiss Society for Biomaterials
DOI: 10.22203/ecm.v041a46

EX VIVO INTERVERTEBRAL DISC CULTURES:DEGENERATION-INDUCTION METHODS AND THEIRIMPLICATIONS FOR CLINICAL TRANSLATION

Autores: E Salzer1 , T.C. Schmitz1 , V.H.M. Mouser1 , A. Vernengo2 , B. Gantenbein3,4, J.U. Jansen5 , C. Neidlinger-Wilke5 , H-J. Wilke5 , S. Grad2 , C.L. Le Maitre6 , M.A. Tryfonidou7 and K. Ito1 *
Publicado en: European Cells and Materials, 2023, ISSN 1473-2262
Editor: Swiss Society for Biomaterials
DOI: 10.22203/ecm.v045a07

One-pot precipitation polymerisation strategy for tuneable injectable Laponite®-pNIPAM hydrogels: Polymerisation, processability and beyond

Autores: Victoria L.Boyes, RonakJanani, SimonPartridge, Lee A.Fielding, Christopher Breen, Jonathan Foulkes, Christine L.Le Maitre, Chris Sammon
Publicado en: Polymer, Edición 233, 2021, ISSN 0032-3861
Editor: Elsevier BV
DOI: 10.1016/j.polymer.2021.124201

An insight on the N-glycome of notochordal cell-rich porcine nucleus pulposus during maturation

Autores: Büşra Günay, Elizabeth Matthews, Jack Morgan, Marianna A. Tryfonidou, Radka Saldova, Abhay Pandit
Publicado en: FASEB BioAdvances, 2023, ISSN 2573-9832
Editor: FASEB
DOI: 10.1096/fba.2023-00011

Non-viral Gene Delivery Methods for Bone and Joints

Autores: Gantenbein, Benjamin; Tang, Shirley; Guerrero, Julien; Higuita-Castro, Natalia; Salazar-Puerta, Ana I.; Croft, Andreas S.; Gazdhar, Amiq; Purmessur, Devina
Publicado en: Frontiers in Bioengineering and Biotechnology, Edición 1, 2020, ISSN 2296-4185
Editor: Frontiers Media S.A.
DOI: 10.3389/fbioe.2020.598466

Epidemiology of Modic changes in dogs: Prevalence, possible risk factors, and association with spinal phenotypes

Autores: Martijn Beukers, Guy C. M. Grinwis, Johannes C. M. Vernooij, Lisanne van der Hoek, Anna R. Tellegen, Björn P. Meij, Stefanie Veraa, Dino Samartzis, Marianna A. Tryfonidou, Frances C. Bach
Publicado en: JOR Spine, 2023, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1273

The effects of 3D culture on the expansion and maintenance of nucleus pulposus progenitor cell multipotency

Autores: Guerrero, Julien; Häckel, Sonja; Croft, Andreas S.; Albers, Christoph E.; Gantenbein, Benjamin
Publicado en: JOR Spine, Edición 1, 2020, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1131

Bioactive potential of natural biomaterials: identification, retention and assessment of biological properties

Autores: Kieran Joyce, Georgina Targa Fabra, Yagmur Bozkurt & Abhay Pandit
Publicado en: Signal Transduction and Targeted Therapy, Edición 6, 2021, ISSN 2059-3635
Editor: Springer Nature
DOI: 10.1038/s41392-021-00512-8

A comprehensive tool box for large animal studies of intervertebral disc degeneration

Autores: Lee, Naomi N.; Salzer, Elias; Bach, Frances C.; Bonilla, Andres F.; Cook, James L.; Gazit, Zulma; Grad, Sybille; Ito, Keita; Smith, Lachlan J.; Vernengo, Andrea; Wilke, Hans-Joachim; Engiles, Julie B.; Tryfonidou, Marianna A.
Publicado en: JOR Spine, 2021, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1162

Mesenchymal Stem Cell-Derived Exosomes: The New Frontier for the Treatment of Intervertebral Disc Degeneration

Autores: Tilotta, Veronica; Vadala, Gianluca; Ambrosio, Luca; Russo, Fabrizio; Cicione, Claudia; Di Giacomo, Giuseppina; Papalia, Rocco; Denaro, Vincenzo
Publicado en: Applied Sciences, 2021, ISSN 2076-3417
Editor: MDPI
DOI: 10.3390/app112311222

New Frontiers towards Regeneration of the Intervertebral Disc: On Progenitor Cells, Growth Factors and Biomaterials

Autores: Benjamin Gantenbein
Publicado en: Applied Sciences, 2021, ISSN 2076-3417
Editor: MDPI
DOI: 10.3390/app112411913

Effect of different cryopreservation media on human nucleus pulposus cells' viability and trilineage potential

Autores: Andreas S. Croft, Julien Guerrero, Katharina A. C. Oswald, Sonja Häckel, Christoph E. Albers, Benjamin Gantenbein
Publicado en: JOR SPINE, 2021, ISSN 2572-1143
Editor: Wiley
DOI: 10.1002/jsp2.1140

Recommendations for intervertebral disc notochordal cell investigation: From isolation to characterization

Autores: Rebecca J. Williams, Lisanne T. Laagland, Frances C. Bach, Lizzy Ward, Wilson Chan, Vivian Tam, Adel Medzikovic, Shaghayegh Basatvat, Lily Paillat, Nicolas Vedrenne, Joseph W. Snuggs, Deepani W. Poramba-Liyanage, Judith A. Hoyland, Danny Chan, Anne Camus, Stephen M. Richardson, Marianna A. Tryfonidou, Christine L. Le Maitre
Publicado en: JOR Spine, 2023, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1272

Hyperosmolar expansion medium improves nucleus pulposus cell phenotype

Autores: Laagland, Lisanne T; Bach, Frances C; Creemers, Laura B; Le Maitre, Christine L; Poramba-Liyanage, Deepani W; Tryfonidou, Marianna A; Chirurgie; CS_Locomotion
Publicado en: JOR Spine, Edición 10, 2022, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1219

Issues with Tissues: Trends in Tissue-Engineered Products in Clinical Trials in the European Union

Autores: Kieran Joyce, Zaklina Buljovcic, Goran Rosic, Marietta Kaszkin-Bettag, and Abhay Pandit
Publicado en: Tissue Engineering Part B, 2023, ISSN 2152-4955
Editor: Mary Ann Liebert Incorporated Publishers
DOI: 10.1089/ten.teb.2022.0094

Modeling policy development: examining national governance of stem cell-based embryo models

Autores: Morris Fabbri, Margaret Ginoza, Lars Assen, Karin Jongsma & Rosario Isasi
Publicado en: Regnerative Medicine, 2023, ISSN 2613-5914
Editor: Future Medicine
DOI: 10.2217/rme-2022-0136

Using responsibility as a strategy

Autores: Assen, LS; Jongsma, KR; Isasi, R; Utomo, L; Tryfonidou, MA; Bredenoord, AL; Chirurgie; CS_Locomotion
Publicado en: Regenerative Medicine, 18(3). Future Medicine Ltd., Edición 7, 2023, ISSN 2613-5914
Editor: Future Medicine
DOI: 10.2217/rme-2022-0187

Fluorescence-Activated Cell Sorting Is More Potent to Fish Intervertebral Disk Progenitor Cells Than Magnetic and Beads-Based Methods

Autores: Daniela A. Frauchiger, Adel Tekari, Rahel D. May, Emina Džafo, Samantha C.W. Chan, Jivko Stoyanov, Alessandro Bertolo, Xingshuo Zhang, Julien Guerrero, Daisuke Sakai, Jordy Schol, Sibylle Grad, Marianna Tryfonidou, Lorin M. Benneker, Benjamin Gantenbein
Publicado en: Tissue Engineering Part C: Methods, Edición 25/10, 2019, Página(s) 571-580, ISSN 1937-3384
Editor: Mary Ann Liebert Inc.
DOI: 10.1089/ten.tec.2018.0375

Hedgehog proteins and parathyroid hormone‐related protein are involved in intervertebral disc maturation, degeneration, and calcification

Autores: Frances C. Bach, Kim M. Rooij, Frank M. Riemers, Joseph W. Snuggs, Willem A. M. Jong, Ying Zhang, Laura B. Creemers, Danny Chan, Christine Le Maitre, Marianna A. Tryfonidou
Publicado en: JOR SPINE, Edición 2/4, 2019, ISSN 2572-1143
Editor: Wiley
DOI: 10.1002/jsp2.1071

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues

Autores: Abbie Binch, Joseph Snuggs, Christine L. Le Maitre
Publicado en: JOR SPINE, 2020, ISSN 2572-1143
Editor: Wiley
DOI: 10.1002/jsp2.1098

NOTO Transcription Factor Directs Human Induced Pluripotent Stem Cell-Derived Mesendoderm Progenitors to a Notochordal Fate

Autores: Pauline Colombier, Boris Halgand, Claire Chédeville, Caroline Chariau, Valentin François-Campion, Stéphanie Kilens, Nicolas Vedrenne, Johann Clouet, Laurent David, Jérôme Guicheux, Anne Camus
Publicado en: Cells, Edición 9/2, 2020, Página(s) 509, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/cells9020509

Moral imagination as an instrument for ethics education for biomedical researchers

Autores: Elianne M. Gerrits, Lars S. Assen, Liesbeth Noordegraaf-Eelens, Annelien L. Bredenoord & Marc H. W. van Mil
Publicado en: International Journal of Ethics Education, 2023, ISSN 2364-0006
Editor: Springer
DOI: 10.1007/s40889-023-00171-z 

A Combined Western and Bead-Based Multiplex Platform to Characterize Extracellular Vesicles

Autores: Josette C. van Maanen, Frances C. Bach, Theresa S. Braun, Alberta Giovanazzi, Bas W.M. van Balkom, Markus Templin, Marca H.M. Wauben, and Marianna A. Tryfonidou
Publicado en: Tissue Engineer Part C, 2023, ISSN 2152-4955
Editor: Mary Ann Liebert Incorporated Publishers
DOI: 10.1089/ten.tec.2023.0056

A bovine nucleus pulposus explant culture model

Autores: Salzer, Elias; Mouser, Vivian H.M.; Tryfonidou, Marianna A.; Ito, Keita
Publicado en: Journal of Orthopaedic Research, 2021, ISSN 1554-527X
Editor: Wiley-Blackwell
DOI: 10.1002/jor.25226

Context-aware modeling for knowledge-intensive medicinal product development processes

Autores: Zeynep Ozturk Yurt, Rik Eshuis, Anna Wilbik & Irene Vanderfeesten
Publicado en: Software and Systems Modeling, 2023, ISSN 1619-1374
Editor: Springer Link
DOI: 10.1007/s10270-022-01070-5

Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells

Autores: Waleed M Kholosy, Marieke Visscher, Kim Ogink, Helen Buttstedt, Kelli Griffin, Axel Beier, Jan Patrick Gerlach, Jan J Molenaar, Niels Geijsen, Marco de Boer, Anna Chatsisvili
Publicado en: Journal of Biotechnology, Edición 338, 2021, Página(s) 71-80, ISSN 0168-1656
Editor: Elsevier BV
DOI: 10.1016/j.jbiotec.2021.07.006

Characterization of biomaterials intended for use in the nucleus pulposus of degenerated intervertebral discs

Autores: Tara C. Schmitz, Elias Salzer, João F. Crispim, Georgina Targa Fabra, Catherine LeVisage, Abhay Pandit, Marianna Tryfonidou, Christine Le Maitre, Keita Ito
Publicado en: Acta Biomaterialia, Edición 114, 2020, Página(s) 1-15, ISSN 1742-7061
Editor: Elsevier BV
DOI: 10.1016/j.actbio.2020.08.001

Influence of Angiopoietin Treatment with Hypoxia and Normoxia on Human Intervertebral Disc Progenitor Cell's Proliferation, Metabolic Activity, and Phenotype

Autores: Bischof, Muriel C.; Häckel, Sonja; Oberli, Andrea; Croft, Andreas S.; Oswald, Katharina A.C.; Albers, Christoph E.; Gantenbein, Benjamin; Guerrero, Julien
Publicado en: Applied Sciences, 2021, ISSN 2076-3417
Editor: MDPI
DOI: 10.3390/app11157144

Development of a standardized histopathology scoring system for human intervertebral disc degeneration: an Orthopaedic Research Society Spine Section Initiative

Autores: Le Maitre, Christine C.; Dahia, Chitra L.; Giers, Morgan; Illien-Junger, Svenja; Cicione, Claudia; Samartzis, Dino; Vadala, Gianluca; Fields, Aaron; Lotz, Jeffrey
Publicado en: JOR Spine, 2021, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1167

Recognizing the ethical implications of stem cell research: A call for broadening the scope

Autores: Assen, L.S.; Jongsma, K.R.; Isasi, R.; Tryfonidou, M.A.; Bredenoord, A.L.
Publicado en: Stem Cell Reports, Edición 16, 2021, Página(s) 1656-1661, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2021.05.021

Dynamic loading leads to increased metabolic activity and spatial redistribution of viable cell density in nucleus pulposus tissue

Autores: Elias Salzer; Vivian H. M. Mouser; Jurgen A. Bulsink; Marianna A. Tryfonidou; Keita Ito
Publicado en: JOR Spine, Edición 5, 2023, ISSN 2572-1143
Editor: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1240

Towards Tissue-Specific Stem Cell Therapy for the Intervertebral Disc: PPARδ Agonist Increases the Yield of Human Nucleus Pulposus Progenitor Cells in Expansion

Autores: Xingshuo Zhang, Julien Guerrero, Andreas S. Croft, Katharina A.C. Oswald, Christoph E. Albers, Sonja Häckel, Benjamin Gantenbein
Publicado en: Surgeries, Edición 2/1, 2021, Página(s) 92-104, ISSN 2673-4095
Editor: MDPI
DOI: 10.3390/surgeries2010008

Monographic books (1)

Intervertebral Disc Regeneration II

Autores: Benjamin Gantenbein
Publicado en: 2023
Editor: MDPI
DOI: 10.3390/books978-3-0365-8199-6

Non-peer reviewed articles (1)

Injectable Hydrogels for Articular Cartilage and Nucleus Pulposus Repair: Status Quo and Prospects

Autores: Bram Zoetebier; Tara C Schmitz; Keita Ito; Marcel Karperien; Marianna A Tryfonidou; Julieta Paez
Publicado en: Tissue Engineering, Edición 16, 2022, ISSN 2152-4955
Editor: Mary Ann Liebert Incorporated Publishers
DOI: 10.1089/ten.tea.2021.0226

Conference proceedings (1)

BPM Support for Regulatory Compliance in ATMP Development Processes

Autores: Ozturk Yurt, Zeynep
Publicado en: CEUR Workshop Proceedings, Edición 2973, 2021
Editor: CEUR-WS