Induced pluripotent stem cell-based therapy for spinal regeneration

Informazioni relative al progetto

iPSpine

ID dell’accordo di sovvenzione: 825925

DOI

10.3030/825925

Progetto chiuso

Data della firma CE 7 Dicembre 2018

Data di avvio 1 Gennaio 2019

Data di completamento 30 Giugno 2024

Finanziato da

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Costo totale

€ 15 396 031,25

Contributo UE

€ 14 959 781,25

Coordinato da

UNIVERSITEIT UTRECHT
Netherlands

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (4)

Updated Dissemination and Exploitation plan
Set up of Patient Advisory Board
Guidelines and requirements for pre-in vivo evaluation of IVD advanced therapeutics

Guidelines and requirements for prein vivo evaluation of IVD advanced therapeutics

Report to the PSC on Dissemination and Education activities

Open Research Data Pilot (1)

Data Management Plan

Other (1)

Optimized open-access knowledge platform available

Websites, patent fillings, videos etc. (2)

Project newsletter
Project website

Pubblicazioni

Peer reviewed articles (38)

Spheroid-Like Cultures for Expanding Angiopoietin Receptor-1 (aka. Tie2) Positive Cells from the Human Intervertebral Disc

Autori: Xingshuo Zhang, Julien Guerrero, Andreas S. Croft, Christoph E. Albers, Sonja Häckel, Benjamin Gantenbein
Pubblicato in: International Journal of Molecular Sciences, Numero 21/24, 2020, Pagina/e 9423, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21249423

Roles and responsibilities in stem cell research: a focus group study with stem cell researchers and patients

Autori: Lars S Assen, Karin R Jongsma, Rosario Isasi, Marianna A Tryfonidou & Annelien L Bredenoord
Pubblicato in: Regenerative Medicine, 2022, ISSN 2613-5914
Editore: Future Medicine
DOI: 10.2217/rme-2022-0019

Harmonization and standardization of nucleus pulposus cell extraction and culture methods

Autori: Shaghayegh Basatvat, Frances C. Bach, Marcos N. Barcellona, Abbie L. Binch, Conor T. Buckley, Brian Bueno, Nadeen O. Chahine, Ana Chee, Laura B. Creemers, Stefan Dudli, Bailey Fearing, Stephen J. Ferguson, Jennifer Gansau, Benjamin Gantenbein, Rahul Gawri, Juliane D. Glaeser, Sibylle Grad, Julien Guerrero, Lisbet Haglund, Paula A. Hernandez, Judith A. Hoyland, Charles Huang, James C. Iatridis, Sve
Pubblicato in: JOR Spine, 2023, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1238

Detergent-Free Decellularization of Notochordal Cell-Derived Matrix Yields a Regenerative, Injectable, and Swellable Biomaterial

Autori: Tara C. Schmitz; Marina van Doeselaar; Marianna A. Tryfonidou; Keita Ito
Pubblicato in: Tissue Engineering and Regenerative Medicine, Numero 10, 2022, ISSN 1932-7005
Editore: ACS Publications
DOI: 10.1021/acsbiomaterials.2c00790

Cell sources proposed for nucleus pulposus regeneration

Autori: Williams, Rebecca J.; Tryfonidou, Marianna A.; Snuggs, Joseph William; Le Maitre, Christine Lyn
Pubblicato in: JOR Spine, 2021, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1175

Notochordal Cell-Based Treatment Strategies and Their Potential in Intervertebral Disc Regeneration

Autori: Frances C Bach 1, Deepani W Poramba-Liyanage 1, Frank M Riemers 1, Jerome Guicheux 2 3 4, Anne Camus 2, James C Iatridis 5, Danny Chan 6, Keita Ito 7 8, Christine L Le Maitre 9, Marianna A Tryfonidou 1
Pubblicato in: Front Cell Dev Biol, 2021, ISSN 2296-634X
Editore: Frontiers
DOI: 10.3389/fcell.2021.780749

The nucleus pulposus microenvironment in the intervertebral disc: the fountain of youth?

Autori: Guerrero, J.; Häckel, S.; Croft, A.S.; Hoppe, S.; Albers, C.E.; Gantenbein, B.
Pubblicato in: eCM (Eur Cell Mater / e Cells & Materials), Numero 41, 2021, Pagina/e 707-738, ISSN 1473-2262
Editore: Swiss Society for Biomaterials
DOI: 10.22203/ecm.v041a46

EX VIVO INTERVERTEBRAL DISC CULTURES:DEGENERATION-INDUCTION METHODS AND THEIRIMPLICATIONS FOR CLINICAL TRANSLATION

Autori: E Salzer1 , T.C. Schmitz1 , V.H.M. Mouser1 , A. Vernengo2 , B. Gantenbein3,4, J.U. Jansen5 , C. Neidlinger-Wilke5 , H-J. Wilke5 , S. Grad2 , C.L. Le Maitre6 , M.A. Tryfonidou7 and K. Ito1 *
Pubblicato in: European Cells and Materials, 2023, ISSN 1473-2262
Editore: Swiss Society for Biomaterials
DOI: 10.22203/ecm.v045a07

One-pot precipitation polymerisation strategy for tuneable injectable Laponite®-pNIPAM hydrogels: Polymerisation, processability and beyond

Autori: Victoria L.Boyes, RonakJanani, SimonPartridge, Lee A.Fielding, Christopher Breen, Jonathan Foulkes, Christine L.Le Maitre, Chris Sammon
Pubblicato in: Polymer, Numero 233, 2021, ISSN 0032-3861
Editore: Elsevier BV
DOI: 10.1016/j.polymer.2021.124201

An insight on the N-glycome of notochordal cell-rich porcine nucleus pulposus during maturation

Autori: Büşra Günay, Elizabeth Matthews, Jack Morgan, Marianna A. Tryfonidou, Radka Saldova, Abhay Pandit
Pubblicato in: FASEB BioAdvances, 2023, ISSN 2573-9832
Editore: FASEB
DOI: 10.1096/fba.2023-00011

Non-viral Gene Delivery Methods for Bone and Joints

Autori: Gantenbein, Benjamin; Tang, Shirley; Guerrero, Julien; Higuita-Castro, Natalia; Salazar-Puerta, Ana I.; Croft, Andreas S.; Gazdhar, Amiq; Purmessur, Devina
Pubblicato in: Frontiers in Bioengineering and Biotechnology, Numero 1, 2020, ISSN 2296-4185
Editore: Frontiers Media S.A.
DOI: 10.3389/fbioe.2020.598466

Epidemiology of Modic changes in dogs: Prevalence, possible risk factors, and association with spinal phenotypes

Autori: Martijn Beukers, Guy C. M. Grinwis, Johannes C. M. Vernooij, Lisanne van der Hoek, Anna R. Tellegen, Björn P. Meij, Stefanie Veraa, Dino Samartzis, Marianna A. Tryfonidou, Frances C. Bach
Pubblicato in: JOR Spine, 2023, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1273

The effects of 3D culture on the expansion and maintenance of nucleus pulposus progenitor cell multipotency

Autori: Guerrero, Julien; Häckel, Sonja; Croft, Andreas S.; Albers, Christoph E.; Gantenbein, Benjamin
Pubblicato in: JOR Spine, Numero 1, 2020, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1131

Bioactive potential of natural biomaterials: identification, retention and assessment of biological properties

Autori: Kieran Joyce, Georgina Targa Fabra, Yagmur Bozkurt & Abhay Pandit
Pubblicato in: Signal Transduction and Targeted Therapy, Numero 6, 2021, ISSN 2059-3635
Editore: Springer Nature
DOI: 10.1038/s41392-021-00512-8

A comprehensive tool box for large animal studies of intervertebral disc degeneration

Autori: Lee, Naomi N.; Salzer, Elias; Bach, Frances C.; Bonilla, Andres F.; Cook, James L.; Gazit, Zulma; Grad, Sybille; Ito, Keita; Smith, Lachlan J.; Vernengo, Andrea; Wilke, Hans-Joachim; Engiles, Julie B.; Tryfonidou, Marianna A.
Pubblicato in: JOR Spine, 2021, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1162

Mesenchymal Stem Cell-Derived Exosomes: The New Frontier for the Treatment of Intervertebral Disc Degeneration

Autori: Tilotta, Veronica; Vadala, Gianluca; Ambrosio, Luca; Russo, Fabrizio; Cicione, Claudia; Di Giacomo, Giuseppina; Papalia, Rocco; Denaro, Vincenzo
Pubblicato in: Applied Sciences, 2021, ISSN 2076-3417
Editore: MDPI
DOI: 10.3390/app112311222

New Frontiers towards Regeneration of the Intervertebral Disc: On Progenitor Cells, Growth Factors and Biomaterials

Autori: Benjamin Gantenbein
Pubblicato in: Applied Sciences, 2021, ISSN 2076-3417
Editore: MDPI
DOI: 10.3390/app112411913

Effect of different cryopreservation media on human nucleus pulposus cells' viability and trilineage potential

Autori: Andreas S. Croft, Julien Guerrero, Katharina A. C. Oswald, Sonja Häckel, Christoph E. Albers, Benjamin Gantenbein
Pubblicato in: JOR SPINE, 2021, ISSN 2572-1143
Editore: Wiley
DOI: 10.1002/jsp2.1140

Recommendations for intervertebral disc notochordal cell investigation: From isolation to characterization

Autori: Rebecca J. Williams, Lisanne T. Laagland, Frances C. Bach, Lizzy Ward, Wilson Chan, Vivian Tam, Adel Medzikovic, Shaghayegh Basatvat, Lily Paillat, Nicolas Vedrenne, Joseph W. Snuggs, Deepani W. Poramba-Liyanage, Judith A. Hoyland, Danny Chan, Anne Camus, Stephen M. Richardson, Marianna A. Tryfonidou, Christine L. Le Maitre
Pubblicato in: JOR Spine, 2023, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1272

Hyperosmolar expansion medium improves nucleus pulposus cell phenotype

Autori: Laagland, Lisanne T; Bach, Frances C; Creemers, Laura B; Le Maitre, Christine L; Poramba-Liyanage, Deepani W; Tryfonidou, Marianna A; Chirurgie; CS_Locomotion
Pubblicato in: JOR Spine, Numero 10, 2022, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1219

Issues with Tissues: Trends in Tissue-Engineered Products in Clinical Trials in the European Union

Autori: Kieran Joyce, Zaklina Buljovcic, Goran Rosic, Marietta Kaszkin-Bettag, and Abhay Pandit
Pubblicato in: Tissue Engineering Part B, 2023, ISSN 2152-4955
Editore: Mary Ann Liebert Incorporated Publishers
DOI: 10.1089/ten.teb.2022.0094

Modeling policy development: examining national governance of stem cell-based embryo models

Autori: Morris Fabbri, Margaret Ginoza, Lars Assen, Karin Jongsma & Rosario Isasi
Pubblicato in: Regnerative Medicine, 2023, ISSN 2613-5914
Editore: Future Medicine
DOI: 10.2217/rme-2022-0136

Using responsibility as a strategy

Autori: Assen, LS; Jongsma, KR; Isasi, R; Utomo, L; Tryfonidou, MA; Bredenoord, AL; Chirurgie; CS_Locomotion
Pubblicato in: Regenerative Medicine, 18(3). Future Medicine Ltd., Numero 7, 2023, ISSN 2613-5914
Editore: Future Medicine
DOI: 10.2217/rme-2022-0187

Fluorescence-Activated Cell Sorting Is More Potent to Fish Intervertebral Disk Progenitor Cells Than Magnetic and Beads-Based Methods

Autori: Daniela A. Frauchiger, Adel Tekari, Rahel D. May, Emina Džafo, Samantha C.W. Chan, Jivko Stoyanov, Alessandro Bertolo, Xingshuo Zhang, Julien Guerrero, Daisuke Sakai, Jordy Schol, Sibylle Grad, Marianna Tryfonidou, Lorin M. Benneker, Benjamin Gantenbein
Pubblicato in: Tissue Engineering Part C: Methods, Numero 25/10, 2019, Pagina/e 571-580, ISSN 1937-3384
Editore: Mary Ann Liebert Inc.
DOI: 10.1089/ten.tec.2018.0375

Hedgehog proteins and parathyroid hormone‐related protein are involved in intervertebral disc maturation, degeneration, and calcification

Autori: Frances C. Bach, Kim M. Rooij, Frank M. Riemers, Joseph W. Snuggs, Willem A. M. Jong, Ying Zhang, Laura B. Creemers, Danny Chan, Christine Le Maitre, Marianna A. Tryfonidou
Pubblicato in: JOR SPINE, Numero 2/4, 2019, ISSN 2572-1143
Editore: Wiley
DOI: 10.1002/jsp2.1071

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues

Autori: Abbie Binch, Joseph Snuggs, Christine L. Le Maitre
Pubblicato in: JOR SPINE, 2020, ISSN 2572-1143
Editore: Wiley
DOI: 10.1002/jsp2.1098

NOTO Transcription Factor Directs Human Induced Pluripotent Stem Cell-Derived Mesendoderm Progenitors to a Notochordal Fate

Autori: Pauline Colombier, Boris Halgand, Claire Chédeville, Caroline Chariau, Valentin François-Campion, Stéphanie Kilens, Nicolas Vedrenne, Johann Clouet, Laurent David, Jérôme Guicheux, Anne Camus
Pubblicato in: Cells, Numero 9/2, 2020, Pagina/e 509, ISSN 2073-4409
Editore: MDPI
DOI: 10.3390/cells9020509

Moral imagination as an instrument for ethics education for biomedical researchers

Autori: Elianne M. Gerrits, Lars S. Assen, Liesbeth Noordegraaf-Eelens, Annelien L. Bredenoord & Marc H. W. van Mil
Pubblicato in: International Journal of Ethics Education, 2023, ISSN 2364-0006
Editore: Springer
DOI: 10.1007/s40889-023-00171-z 

A Combined Western and Bead-Based Multiplex Platform to Characterize Extracellular Vesicles

Autori: Josette C. van Maanen, Frances C. Bach, Theresa S. Braun, Alberta Giovanazzi, Bas W.M. van Balkom, Markus Templin, Marca H.M. Wauben, and Marianna A. Tryfonidou
Pubblicato in: Tissue Engineer Part C, 2023, ISSN 2152-4955
Editore: Mary Ann Liebert Incorporated Publishers
DOI: 10.1089/ten.tec.2023.0056

A bovine nucleus pulposus explant culture model

Autori: Salzer, Elias; Mouser, Vivian H.M.; Tryfonidou, Marianna A.; Ito, Keita
Pubblicato in: Journal of Orthopaedic Research, 2021, ISSN 1554-527X
Editore: Wiley-Blackwell
DOI: 10.1002/jor.25226

Context-aware modeling for knowledge-intensive medicinal product development processes

Autori: Zeynep Ozturk Yurt, Rik Eshuis, Anna Wilbik & Irene Vanderfeesten
Pubblicato in: Software and Systems Modeling, 2023, ISSN 1619-1374
Editore: Springer Link
DOI: 10.1007/s10270-022-01070-5

Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells

Autori: Waleed M Kholosy, Marieke Visscher, Kim Ogink, Helen Buttstedt, Kelli Griffin, Axel Beier, Jan Patrick Gerlach, Jan J Molenaar, Niels Geijsen, Marco de Boer, Anna Chatsisvili
Pubblicato in: Journal of Biotechnology, Numero 338, 2021, Pagina/e 71-80, ISSN 0168-1656
Editore: Elsevier BV
DOI: 10.1016/j.jbiotec.2021.07.006

Characterization of biomaterials intended for use in the nucleus pulposus of degenerated intervertebral discs

Autori: Tara C. Schmitz, Elias Salzer, João F. Crispim, Georgina Targa Fabra, Catherine LeVisage, Abhay Pandit, Marianna Tryfonidou, Christine Le Maitre, Keita Ito
Pubblicato in: Acta Biomaterialia, Numero 114, 2020, Pagina/e 1-15, ISSN 1742-7061
Editore: Elsevier BV
DOI: 10.1016/j.actbio.2020.08.001

Influence of Angiopoietin Treatment with Hypoxia and Normoxia on Human Intervertebral Disc Progenitor Cell's Proliferation, Metabolic Activity, and Phenotype

Autori: Bischof, Muriel C.; Häckel, Sonja; Oberli, Andrea; Croft, Andreas S.; Oswald, Katharina A.C.; Albers, Christoph E.; Gantenbein, Benjamin; Guerrero, Julien
Pubblicato in: Applied Sciences, 2021, ISSN 2076-3417
Editore: MDPI
DOI: 10.3390/app11157144

Development of a standardized histopathology scoring system for human intervertebral disc degeneration: an Orthopaedic Research Society Spine Section Initiative

Autori: Le Maitre, Christine C.; Dahia, Chitra L.; Giers, Morgan; Illien-Junger, Svenja; Cicione, Claudia; Samartzis, Dino; Vadala, Gianluca; Fields, Aaron; Lotz, Jeffrey
Pubblicato in: JOR Spine, 2021, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1167

Recognizing the ethical implications of stem cell research: A call for broadening the scope

Autori: Assen, L.S.; Jongsma, K.R.; Isasi, R.; Tryfonidou, M.A.; Bredenoord, A.L.
Pubblicato in: Stem Cell Reports, Numero 16, 2021, Pagina/e 1656-1661, ISSN 2213-6711
Editore: Cell Press
DOI: 10.1016/j.stemcr.2021.05.021

Dynamic loading leads to increased metabolic activity and spatial redistribution of viable cell density in nucleus pulposus tissue

Autori: Elias Salzer; Vivian H. M. Mouser; Jurgen A. Bulsink; Marianna A. Tryfonidou; Keita Ito
Pubblicato in: JOR Spine, Numero 5, 2023, ISSN 2572-1143
Editore: Wiley-Blackwell Publishing Ltd
DOI: 10.1002/jsp2.1240

Towards Tissue-Specific Stem Cell Therapy for the Intervertebral Disc: PPARδ Agonist Increases the Yield of Human Nucleus Pulposus Progenitor Cells in Expansion

Autori: Xingshuo Zhang, Julien Guerrero, Andreas S. Croft, Katharina A.C. Oswald, Christoph E. Albers, Sonja Häckel, Benjamin Gantenbein
Pubblicato in: Surgeries, Numero 2/1, 2021, Pagina/e 92-104, ISSN 2673-4095
Editore: MDPI
DOI: 10.3390/surgeries2010008

Monographic books (1)

Intervertebral Disc Regeneration II

Autori: Benjamin Gantenbein
Pubblicato in: 2023
Editore: MDPI
DOI: 10.3390/books978-3-0365-8199-6

Non-peer reviewed articles (1)

Injectable Hydrogels for Articular Cartilage and Nucleus Pulposus Repair: Status Quo and Prospects

Autori: Bram Zoetebier; Tara C Schmitz; Keita Ito; Marcel Karperien; Marianna A Tryfonidou; Julieta Paez
Pubblicato in: Tissue Engineering, Numero 16, 2022, ISSN 2152-4955
Editore: Mary Ann Liebert Incorporated Publishers
DOI: 10.1089/ten.tea.2021.0226

Conference proceedings (1)

BPM Support for Regulatory Compliance in ATMP Development Processes

Autori: Ozturk Yurt, Zeynep
Pubblicato in: CEUR Workshop Proceedings, Numero 2973, 2021
Editore: CEUR-WS