BIOPRINTING BY LIGHT-SHEET LITHOGRAPHY: ENGINEERING COMPLEX TISSUES WITH HIGH RESOLUTION AT HIGH SPEED

Informazioni relative al progetto

BRIGHTER

ID dell’accordo di sovvenzione: 828931

DOI

10.3030/828931

Progetto chiuso

Data della firma CE 16 Gennaio 2019

Data di avvio 1 Luglio 2019

Data di completamento 31 Dicembre 2022

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 520 720,50

Contributo UE

€ 3 459 052,50

Coordinato da

FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA
Spain

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (12)

BRIGHTER web site and logo

It will include basic project information, logo, news and events, downloadable e-newsletters, and links to social media accounts (tweeter, Facebook and LinkedIn). It is a living document.

Characterization of first batch of hydrogels

Calibration of the crosslinking density and stiffness of hydrogels formed by light-sheet illumination as a function of the exposure dose.

Initial specifications of the pattern generator

Description of the requirements and technical specifications of the pattern generator to be applied to the light-sheet bioprinting system

Photopolymerizable polymers biocompatibility

Experimental procedure and characterization of the functionalization of the photopolymerizable polymers to promote cell adhesion. Evaluation of biocompatibility.

Reconstitution of epidermis and dermis

Development and characterization of a skin samples composed of dermis and epidermis layers including the rete ridge 3D structures

Characterization of reversible crosslinked IPNs hydrogels

Calibration of the crosslinking density and stiffness of reversible crosslinkable and IPNs hydrogels formed by lightsheet illumination as a function of the exposure dose

Report on the light-sheet bioprinter process characteristics

Description of the technical parameters to produce crosslinked hydrogels with the lightsheet illumination set up

Report on networking activities

Framework document for project networking activities. It will outline the connections and initiatives where the project partners are involved to maximize the project results.

Cell viability on IPNs cell-laden hydrogels

Cell viability on cellladen IPNs hydrogels polymerized under lightsheet illumination within the dedicated bioprinting chambers

Cell viability on cell-laden hydrogels

Cell viability on cellladen hydrogels polymerized under lightsheet illumination within the dedicated bioprinting chambers

Report of kick-off meeting

Minutes of the kick-off meeting including the agenda, list of participants with their affiliations and roles, and presentations of participants

Protocols to isolate, culture and characterize relevant skin cells

Protocols describing specific cell preparation to be used in combination with BRIGHTER polymers and device

Other (1)

Project Workshop

Organization of a project workshop, to promote the exchange of PhD students among the consortium members

Pubblicazioni

Thesis and dissertations (2)

A dynamic acousto-optic lithographic patterning technique for bioprinting

Autori: Rosemarie Ohanyan
Pubblicato in: 2022
Editore: KTH Royal Institute of Technology

Evaluation of optical methods for 3D-printing of tissue

Autori: Jim Larsson
Pubblicato in: 2020
Editore: KTH Royal Institute of Technology

Peer reviewed articles (8)

Ephrin Micropatterns Exogenously Modulate Cell Organization in Organoid-Derived Intestinal Epithelial Monolayers

Autori: Enara Larrañaga, Vanesa Fernández-Majada, Samuel Ojosnegros, Jordi Comelles,* and Elena Martinez
Pubblicato in: Adv. Mater. Interfaces, 2022, ISSN 2196-7350
Editore: Weinheim, Germany : Wiley-VCH, 2014-
DOI: 10.1002/admi.202201301

Stem cell responses to stretch and strain

Autori: Swarnabh Bhattacharya , Haguy Wolfenson , Ramesh Shivdasani , Ruby Shalom-Feuerstein 
Pubblicato in: Trends in Cell Biology, 2022, ISSN 1879-3088
Editore: Cambridge, UK : Elsevier Science Publishers, c1991-
DOI: 10.1016/j.tcb.2021.10.007

Multi-Modal and Molecular Imaging of Cellular Microenvironment and Tissue Development

Autori: Francesco Pampaloni
Pubblicato in: International Journal of Molecular Sciences, 2022, ISSN 2572-1100
Editore: Juniper Publishers
DOI: 10.3390/ijms23137113

Upgrading a Consumer Stereolithographic 3D Printer to Produce a Physiologically Relevant Model with Human Liver Cancer Organoids

Autori: Louise Breideband, Kaja N Wächtershäuser, Levin Hafa, Konstantin Wieland, Achilleas S Frangakis, Ernst HK Stelzer, Francesco Pampaloni
Pubblicato in: Advanced Materials Technologies, 2022, ISSN 2365-709X
Editore: Wiley Blackwell
DOI: 10.1002/admt.202200029

Spotlighting adult stem cells: advances, pitfalls, and challenges

Autori: Anna Altshuler, Sara A Wickström, Ruby Shalom-Feuerstein 
Pubblicato in: Trends in Cell Biology, 2022, ISSN 1879-3088
Editore: Cambridge, UK : Elsevier Science Publishers
DOI: 10.1016/j.tcb.2022.09.007

MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity

Autori: Arne Peirsman, Eva Blondeel, Tasdiq Ahmed, Jasper Anckaert, Dominique Audenaert, Tom Boterberg, Krisztina Buzas, Neil Carragher, Gastone Castellani, Flávia Castro, Virginie Dangles-Marie, John Dawson, Pascal De Tullio, Elly De Vlieghere, Sándor Dedeyne, Herman Depypere, Akos Diosdi, Ruslan I. Dmitriev, Helmut Dolznig, Suzanne Fischer, Christian Gespach, Vera Goossens, Jyrki Heino, An Hendrix, Pe
Pubblicato in: Nature Methods, 2021, ISSN 1548-7105
Editore: New York, NY : Nature Pub. Group, c2004-
DOI: 10.1038/s41592-021-01291-4

Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Autori: Lotta Hof, Till Moreth, Michael Koch, Tim Liebisch, Marina Kurtz, Julia Tarnick, Susanna M. Lissek, Monique M. A. Verstegen, Luc J. W. van der Laan, Meritxell Huch, Franziska Matthäus, Ernst H. K. Stelzer & Francesco Pampaloni
Pubblicato in: BMC Biology, 2021, ISSN 1741-7007
Editore: BioMed Central
DOI: 10.1186/s12915-021-00958-w

Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing

Autori: Anna Altshuler, Aya Amitai-Lange, Noam Tarazi, Sunanda Dey, Lior Strinkovsky, Shira Hadad-Porat, Swarnabh Bhattacharya, Waseem Nasser, Jusuf Imeri, Gil Ben-David, Ghada Abboud-Jarrous, Beatrice Tiosano, Eran Berkowitz, Nathan Karin, Yonatan Savir, Ruby Shalom-Feuerstein
Pubblicato in: Cell Stem Cell, 2022, ISSN 1875-9777
Editore: Elsevier
DOI: 10.1016/j.stem.2021.04.003

Non-peer reviewed articles (1)

Capturing limbal epithelial stem cell population dynamics, signature, and their niche

Autori: Anna Altshuler , Aya Amitai-Lange , Noam Tarazi, Sunanda Dey, Lior Strinkovsky, Swarnabh Bhattacharya, Shira Hadad-Porat, Waseem Nasser, Jusuf Imeri, Gil Ben-David, Beatrice Tiosano, Eran Berkowitz, Nathan Karin, Yonatan Savir, Ruby Shalom-Feuerstein
Pubblicato in: bioRxiv, 2020, ISSN 2692-8205
Editore: COLD SPRING HARBOR LABORATORY
DOI: 10.1101/2020.06.30.179754

Diritti di proprietà intellettuale

Other (4)

DEVICE AND METHOD FOR STEREOLITHOGRAPHIC THREE DIMENSIONAL PRINTING

Numero candidatura/pubblicazione: PCT EP2021/072200
Data: 2021-08-09
Candidato/i: JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN

DEVICE AND METHOD FOR STEREOLITHOGRAPHIC THREE DIMENSIONAL PRINTING

Numero candidatura/pubblicazione: PCT EP2021/072200
Data: 2021-08-09
Candidato/i: UNIVERSITAT DE BARCELONA

DEVICE AND METHOD FOR STEREOLITHOGRAPHIC THREE DIMENSIONAL PRINTING

Numero candidatura/pubblicazione: PCT EP2021/072200
Data: 2021-08-09
Candidato/i: MYCRONIC AB

DEVICE AND METHOD FOR STEREOLITHOGRAPHIC THREE DIMENSIONAL PRINTING

Numero candidatura/pubblicazione: PCT EP2021/072200
Data: 2021-08-09
Candidato/i: FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA