Synthetic biology of non-ribosomal peptide synthetases to generate new peptides

Project Information

SYNPEP

Grant agreement ID: 835108

DOI

10.3030/835108

Project closed

EC signature date 30 July 2019

Start date 1 October 2019

End date 30 September 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 3 165 788,00

EU contribution

€ 3 165 788,00

Coordinated by

JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (26)

Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria

Author(s): Yi-Ming Shi Merle Hirschmann Yan-Ni Shi Shabbir Ahmed Desalegne Abebew, Nicholas J. Tobias, Peter Grün, Jan J. Crames, Laura Pöschel, Wolfgang Kuttenlochner, Christian Richter, Jennifer Herrmann, Rolf Müller, Aunchalee Thanwisai, Sacha J. Pidot, Timothy P. Stinear, Michael Groll, Yonggyun Kim Helge B. Bode
Published in: Nature Chemistry, 2022, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-022-00923-2

Evolution-inspired engineering of nonribosomal peptide synthetases

Author(s): Kenan A. J. Bozhueyuek, Leonard Präve, Carsten Kegler, Leonie Schenk, Sebastian Kaiser, Christian Schelhas, Yan-Ni Shi, Wolfgang Kuttenlochner, Max Schreiber, Joshua Kandler, Mohammad Alanjary, T.M. Mohiuddin, Michael Groll, Georg K. A. Hochberg, Helge B. Bode
Published in: Science, Issue 383 (6689), 2024, Page(s) eadg4320, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.adg4320

A Practical Guideline to Engineering Nonribosomal Peptide Synthetases

Author(s): Nadya Abbood, Leonard Praeve, Kenan Bozhüyük, Helge B. Bode
Published in: Methods in Molecular Biology (MIMB), Issue 2670, 2023, Page(s) 219-234, ISSN 1064-3745
Publisher: Humana Press, Inc.
DOI: 10.1007/978-1-0716-3214-7

NMR resonance assignments for a docking domain pair with an attached thiolation domain from the PAX peptide-producing NRPS from Xenorhabdus cabanillasii

Author(s): Jonas Watzel, Sepas Sarawi, Elke Duchardt-Ferner, Helge B. Bode, Jens Wöhnert
Published in: Biomolecular NMR Assignments, Issue 15/1, 2021, Page(s) 229-234, ISSN 1874-2718
Publisher: Springer Verlag
DOI: 10.1007/s12104-021-10010-1

Type S Non-Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries

Author(s): Nadya Abbood Tien Duy Vo Jonas Watzel Kenan A J Bozhueyuek Helge B Bode
Published in: Chem Eur J, Issue 09476539, 2022, Page(s) e202103963, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202103963

Nematode-Associated Bacteria: Production of Antimicrobial Agent as a Presumptive Nominee for Curing Endodontic Infections Caused by Enterococcus faecalis

Author(s): Hicran Donmez Ozkan, Harun Cimen, Derya Ulug, Sebastian Wenski, Senem Yigit Ozer, Murat Telli, Neriman Aydin, Helge B. Bode, Selcuk Hazir
Published in: Frontiers in Microbiology, Issue 10, 2019, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2019.02672

Cooperation between a T Domain and a Minimal C‐Terminal Docking Domain to Enable Specific Assembly in a Multiprotein NRPS

Author(s): Jonas Watzel, Elke Duchardt‐Ferner, Sepas Sarawi, Helge B. Bode, Jens Wöhnert
Published in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202103498

Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Author(s): Mustapha Touray, Harun Cimen, Edna Bode, Helge B. Bode, Selcuk Hazir
Published in: Journal Of Pest Science, Issue 97, 2024, Page(s) 2203-2215, ISSN 1612-4758
Publisher: Springer Verlag
DOI: 10.1007/s10340-024-01760-7

Structure and biosynthesis of deoxy-polyamine in Xenorhabdus bovienii

Author(s): Sebastian L Wenski Natalie Berghaus Nadine Keller Helge B Bode
Published in: J Ind Microbiol Biotechnol, Issue 13675435, 2021, Page(s) kuab006, ISSN 1367-5435
Publisher: Springer Verlag
DOI: 10.1093/jimb/kuab006

Isofunctional but Structurally Different Methyltransferases for Dithiolopyrrolone Diversification

Author(s): Li Su, Eva M. Huber, Margaretha Westphalen, Jonas Gellner, Edna Bode, Tania Köbel, Peter Grün, Mohammad M. Alanjary, Timo Glatter, Katarina Cirnski, Rolf Müller, Daniel Schindler, Michael Groll, Helge B. Bode
Published in: Angewandte Chemie International Edition, 2024, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202410799

A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments

Author(s): Eva M. Huber, Lukas Kreling, Antje K. Heinrich, Maximilian Dünnebacke, Alexander Pöthig, Helge B. Bode, Michael Groll
Published in: Structure, Issue 31 (5), 2023, ISSN 0969-2126
Publisher: Cell Press
DOI: 10.1016/j.str.2023.03.001

Bioengineering of syrbactin megasynthetases for immunoproteasome inhibitor production

Author(s): Leonard Präve, Wolfgang Kuttenlochner, Werner W.A. Tabak, Chiara Langer, Markus Kaiser, Michael Groll, Helge B. Bode
Published in: Chem, Issue 10, 2024, Page(s) 3212-3223, ISSN 2451-9294
Publisher: Cell Press
DOI: 10.1016/j.chempr.2024.07.013

easyPACId, a Simple Method for Induced Production, Isolation, Identification, and Testing of Natural Products from Proteobacteria

Author(s): Edna Bode, Daniela Assmann, Petra Happel, Elmar Meyer, Karin Münch, Nicole Rössel, Helge B. Bode
Published in: bio-protocol, Issue 13(13): e4709, 2023, ISSN 2331-8325
Publisher: Bio-Protocol LLC
DOI: 10.21769/bioprotoc.4709

Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Author(s): Alexander Rill, Lei Zhao, Helge B. Bode
Published in: Microbial Cell Factories, Issue 23 (1), 2024, Page(s) 98, ISSN 1475-2859
Publisher: BioMed Central
DOI: 10.1186/s12934-024-02363-8

Guidelines for Optimizing Type S Nonribosomal Peptide Synthetases

Author(s): Nadya Abbood, Juliana Effert, Kenan A. J. Bozhueyuek, Helge B. Bode
Published in: ACS Synthetic Biology, 2023, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.3c00295

Investigation of the Odilorhabdin Biosynthetic Gene Cluster Using NRPS Engineering

Author(s): Leonard Präve, Carsten E. Seyfert, Kenan A. J. Bozhüyük, Emilie Racine, Rolf Müller, Helge B. Bode
Published in: Angewandte Chemie International Edition, Issue 63, 2024, Page(s) e202406389, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202406389

Engineering of Specific Single-Module Nonribosomal Peptide Synthetases of the RXP Type for the Production of Defined Peptides

Author(s): Xiaofeng Cai, Lei Zhao, Helge B. Bode
Published in: ACS Synthetic Biology, Issue 12 (1), 2023, Page(s) 203-212, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.2c00472

Natural products from Xenorhabdus and Photorhabdus show promise as biolarvicides against Aedes albopictus

Author(s): Mustapha Touray, Derya Ulug, Sebnem Hazal Gulsen, Harun Cimen, Canan Hazir, Helge B. Bode, Selcuk Hazir
Published in: Pest Management Science, 2024, Page(s) 4231-4242, ISSN 1526-498X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/ps.8127

Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii involves cross-talk between specialized and primary metabolism

Author(s): Siyar Kavakli, Gina L.C.Grammbitter, Helge B.Bode
Published in: Tetrahedron, 2022, ISSN 1464-5416
Publisher: Elsevier
DOI: 10.1016/j.tet.2022.133116

Cleavage Off-Loading and Post-assembly-Line Conversions Yield Products with Unusual Termini during Biosynthesis

Author(s): Yi-Ming Shi Merle Hirschmann Yan-Ni Shi Helge B Bode
Published in: ACS Chem Biol, Issue 15548929, 2022, Page(s) 2221-2228, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.2c00367

Synthetic Zippers as an Enabling Tool for Engineering of Non-Ribosomal Peptide Synthetases

Author(s): Kenan A. J. Bozhueyuek; Jonas Watzel; Nadya Abbood; Helge B. Bode
Published in: Angew Chem Int Ed, Issue 1, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1101/2020.05.06.080655

Genome Mining Enabled by Biosynthetic Characterization Uncovers a Class of Benzoxazolinate-Containing Natural Products in Diverse Bacteria

Author(s): Yi-Ming Shi Jan J Crames Laura Czech Kenan A J Bozhüyük Yan-Ni Shi Merle Hirschmann Stefanie Lamberth Peter Claus Nicole Paczia Christian Rückert Jörn Kalinowski Gert Bange Helge B Bode
Published in: Angew Chem Int Ed, 2022, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202206106

Activation, Structure, Biosynthesis and Bioactivity of Glidobactin‐like Proteasome Inhibitors from Photorhabdus laumondii

Author(s): Lei Zhao, Camille Le Chapelain, Alexander O. Brachmann, Marcel Kaiser, Michael Groll, Helge B. Bode
Published in: ChemBioChem, Issue 22/9, 2021, Page(s) 1582-1588, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202100014

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Author(s): Andreas Tietze, Yan‐Ni Shi, Max Kronenwerth, Helge B. Bode
Published in: ChemBioChem, Issue 21/19, 2020, Page(s) 2750-2754, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000176

Integrating genomics and metabolomics for scalable non-ribosomal peptide discovery

Author(s): Bahar Behsaz, Edna Bode, Alexey Gurevich, Yan-Ni Shi, Florian Grundmann, Deepa Acharya, Andrés Mauricio Caraballo-Rodríguez, Amina Bouslimani, Morgan Panitchpakdi, Annabell Linck, Changhui Guan, Julia Oh, Pieter C. Dorrestein, Helge B. Bode, Pavel A. Pevzner, Hosein Mohimani
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-23502-4

Microbial Cationic Peptides as a Natural Defense Mechanism against Insect Antimicrobial Peptides

Author(s): Tien Duy Vo, Christoph Spahn, Mike Heilemann, Helge B. Bode
Published in: ACS Chemical Biology, Issue 16/3, 2021, Page(s) 447-451, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.0c00794

Non-peer reviewed articles (4)

Influence of condensation domains on activity and specificity of adenylation domains

Author(s): Janik Kranz, Sebastian L. Wenski, Alexander A. Dichter, Helge B. Bode, Kenan A. J. Bozhüyük
Published in: bioRxiv, 2021, ISSN 2692-8205
Publisher: Cold Spring Harbor
DOI: 10.1101/2021.08.23.457306

Polyketide synthase-derived sphingolipids determine microbiota-mediated protection against pathogens in C. elegans

Author(s): Lena Peters, Moritz Drechsler, Barbara Pees, Georgia Angelidou, Liesa Salzer, Karlis Arturs Moors, Nicole Paczia, Hinrich Schulenburg, Yi-Ming Shi, Christoph Kaleta, Michael Witting, Helge B. Bode, Katja Dierking
Published in: bioRxiv, 2024, ISSN 0362-4331
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.02.06.579051

Synthetische Zipper für die Biosynthese neuer Naturstoffderivate

Author(s): Nadya Abbood Kenan A.J. Bozhüyük Helge B. Bode
Published in: Biospektrum, Issue 28, 2022, Page(s) 446-449, ISSN 0947-0867
Publisher: Spektrum Akademischer Verlag GmbH
DOI: 10.1007/s12268-022-1785-7

The Chemical Structure of Widespread Microbial Aryl Polyene Lipids

Author(s): Gina L. C. Grammbitter, Yi-Ming Shi, Yan-Ni Shi, Sahithya P. B. Vemulapalli, Christian Richter, Harald Schwalbe, Mohammad Alanjary, Anja Schüffler, Matthias Witt, Christian Griesinger, Helge B. Bode
Published in: bioRxiv, 2020, ISSN 2692-8205
Publisher: Cold Spring Harbor
DOI: 10.1101/2020.12.19.423268

Intellectual Property Rights

Patent (1)

ADAPTER SYSTEM OF ARTIFICIAL NON-RIBOSOMAL PEPTIDE SYNTHETASES AND POLYKETIDE SYNTHASES

Application/Publication number: 19 208603
Date: 2019-11-12
Applicant(s): JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN