Synthetic biology of non-ribosomal peptide synthetases to generate new peptides

Información del proyecto

SYNPEP

Identificador del acuerdo de subvención: 835108

DOI

10.3030/835108

Proyecto cerrado

Fecha de la firma de la CE 30 Julio 2019

Fecha de inicio 1 Octubre 2019

Fecha de finalización 30 Septiembre 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 3 165 788,00

Aportación de la UE

€ 3 165 788,00

Coordinado por

JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Germany

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (26)

Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria

Autores: Yi-Ming Shi Merle Hirschmann Yan-Ni Shi Shabbir Ahmed Desalegne Abebew, Nicholas J. Tobias, Peter Grün, Jan J. Crames, Laura Pöschel, Wolfgang Kuttenlochner, Christian Richter, Jennifer Herrmann, Rolf Müller, Aunchalee Thanwisai, Sacha J. Pidot, Timothy P. Stinear, Michael Groll, Yonggyun Kim Helge B. Bode
Publicado en: Nature Chemistry, 2022, ISSN 1755-4330
Editor: Nature Publishing Group
DOI: 10.1038/s41557-022-00923-2

Evolution-inspired engineering of nonribosomal peptide synthetases

Autores: Kenan A. J. Bozhueyuek, Leonard Präve, Carsten Kegler, Leonie Schenk, Sebastian Kaiser, Christian Schelhas, Yan-Ni Shi, Wolfgang Kuttenlochner, Max Schreiber, Joshua Kandler, Mohammad Alanjary, T.M. Mohiuddin, Michael Groll, Georg K. A. Hochberg, Helge B. Bode
Publicado en: Science, Edición 383 (6689), 2024, Página(s) eadg4320, ISSN 0036-8075
Editor: American Association for the Advancement of Science
DOI: 10.1126/science.adg4320

A Practical Guideline to Engineering Nonribosomal Peptide Synthetases

Autores: Nadya Abbood, Leonard Praeve, Kenan Bozhüyük, Helge B. Bode
Publicado en: Methods in Molecular Biology (MIMB), Edición 2670, 2023, Página(s) 219-234, ISSN 1064-3745
Editor: Humana Press, Inc.
DOI: 10.1007/978-1-0716-3214-7

NMR resonance assignments for a docking domain pair with an attached thiolation domain from the PAX peptide-producing NRPS from Xenorhabdus cabanillasii

Autores: Jonas Watzel, Sepas Sarawi, Elke Duchardt-Ferner, Helge B. Bode, Jens Wöhnert
Publicado en: Biomolecular NMR Assignments, Edición 15/1, 2021, Página(s) 229-234, ISSN 1874-2718
Editor: Springer Verlag
DOI: 10.1007/s12104-021-10010-1

Type S Non-Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries

Autores: Nadya Abbood Tien Duy Vo Jonas Watzel Kenan A J Bozhueyuek Helge B Bode
Publicado en: Chem Eur J, Edición 09476539, 2022, Página(s) e202103963, ISSN 0947-6539
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202103963

Nematode-Associated Bacteria: Production of Antimicrobial Agent as a Presumptive Nominee for Curing Endodontic Infections Caused by Enterococcus faecalis

Autores: Hicran Donmez Ozkan, Harun Cimen, Derya Ulug, Sebastian Wenski, Senem Yigit Ozer, Murat Telli, Neriman Aydin, Helge B. Bode, Selcuk Hazir
Publicado en: Frontiers in Microbiology, Edición 10, 2019, ISSN 1664-302X
Editor: Frontiers Media
DOI: 10.3389/fmicb.2019.02672

Cooperation between a T Domain and a Minimal C‐Terminal Docking Domain to Enable Specific Assembly in a Multiprotein NRPS

Autores: Jonas Watzel, Elke Duchardt‐Ferner, Sepas Sarawi, Helge B. Bode, Jens Wöhnert
Publicado en: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202103498

Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Autores: Mustapha Touray, Harun Cimen, Edna Bode, Helge B. Bode, Selcuk Hazir
Publicado en: Journal Of Pest Science, Edición 97, 2024, Página(s) 2203-2215, ISSN 1612-4758
Editor: Springer Verlag
DOI: 10.1007/s10340-024-01760-7

Structure and biosynthesis of deoxy-polyamine in Xenorhabdus bovienii

Autores: Sebastian L Wenski Natalie Berghaus Nadine Keller Helge B Bode
Publicado en: J Ind Microbiol Biotechnol, Edición 13675435, 2021, Página(s) kuab006, ISSN 1367-5435
Editor: Springer Verlag
DOI: 10.1093/jimb/kuab006

Isofunctional but Structurally Different Methyltransferases for Dithiolopyrrolone Diversification

Autores: Li Su, Eva M. Huber, Margaretha Westphalen, Jonas Gellner, Edna Bode, Tania Köbel, Peter Grün, Mohammad M. Alanjary, Timo Glatter, Katarina Cirnski, Rolf Müller, Daniel Schindler, Michael Groll, Helge B. Bode
Publicado en: Angewandte Chemie International Edition, 2024, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202410799

A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments

Autores: Eva M. Huber, Lukas Kreling, Antje K. Heinrich, Maximilian Dünnebacke, Alexander Pöthig, Helge B. Bode, Michael Groll
Publicado en: Structure, Edición 31 (5), 2023, ISSN 0969-2126
Editor: Cell Press
DOI: 10.1016/j.str.2023.03.001

Bioengineering of syrbactin megasynthetases for immunoproteasome inhibitor production

Autores: Leonard Präve, Wolfgang Kuttenlochner, Werner W.A. Tabak, Chiara Langer, Markus Kaiser, Michael Groll, Helge B. Bode
Publicado en: Chem, Edición 10, 2024, Página(s) 3212-3223, ISSN 2451-9294
Editor: Cell Press
DOI: 10.1016/j.chempr.2024.07.013

easyPACId, a Simple Method for Induced Production, Isolation, Identification, and Testing of Natural Products from Proteobacteria

Autores: Edna Bode, Daniela Assmann, Petra Happel, Elmar Meyer, Karin Münch, Nicole Rössel, Helge B. Bode
Publicado en: bio-protocol, Edición 13(13): e4709, 2023, ISSN 2331-8325
Editor: Bio-Protocol LLC
DOI: 10.21769/bioprotoc.4709

Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Autores: Alexander Rill, Lei Zhao, Helge B. Bode
Publicado en: Microbial Cell Factories, Edición 23 (1), 2024, Página(s) 98, ISSN 1475-2859
Editor: BioMed Central
DOI: 10.1186/s12934-024-02363-8

Guidelines for Optimizing Type S Nonribosomal Peptide Synthetases

Autores: Nadya Abbood, Juliana Effert, Kenan A. J. Bozhueyuek, Helge B. Bode
Publicado en: ACS Synthetic Biology, 2023, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.3c00295

Investigation of the Odilorhabdin Biosynthetic Gene Cluster Using NRPS Engineering

Autores: Leonard Präve, Carsten E. Seyfert, Kenan A. J. Bozhüyük, Emilie Racine, Rolf Müller, Helge B. Bode
Publicado en: Angewandte Chemie International Edition, Edición 63, 2024, Página(s) e202406389, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202406389

Engineering of Specific Single-Module Nonribosomal Peptide Synthetases of the RXP Type for the Production of Defined Peptides

Autores: Xiaofeng Cai, Lei Zhao, Helge B. Bode
Publicado en: ACS Synthetic Biology, Edición 12 (1), 2023, Página(s) 203-212, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.2c00472

Natural products from Xenorhabdus and Photorhabdus show promise as biolarvicides against Aedes albopictus

Autores: Mustapha Touray, Derya Ulug, Sebnem Hazal Gulsen, Harun Cimen, Canan Hazir, Helge B. Bode, Selcuk Hazir
Publicado en: Pest Management Science, 2024, Página(s) 4231-4242, ISSN 1526-498X
Editor: John Wiley & Sons Inc.
DOI: 10.1002/ps.8127

Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii involves cross-talk between specialized and primary metabolism

Autores: Siyar Kavakli, Gina L.C.Grammbitter, Helge B.Bode
Publicado en: Tetrahedron, 2022, ISSN 1464-5416
Editor: Elsevier
DOI: 10.1016/j.tet.2022.133116

Cleavage Off-Loading and Post-assembly-Line Conversions Yield Products with Unusual Termini during Biosynthesis

Autores: Yi-Ming Shi Merle Hirschmann Yan-Ni Shi Helge B Bode
Publicado en: ACS Chem Biol, Edición 15548929, 2022, Página(s) 2221-2228, ISSN 1554-8929
Editor: American Chemical Society
DOI: 10.1021/acschembio.2c00367

Synthetic Zippers as an Enabling Tool for Engineering of Non-Ribosomal Peptide Synthetases

Autores: Kenan A. J. Bozhueyuek; Jonas Watzel; Nadya Abbood; Helge B. Bode
Publicado en: Angew Chem Int Ed, Edición 1, 2021, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1101/2020.05.06.080655

Genome Mining Enabled by Biosynthetic Characterization Uncovers a Class of Benzoxazolinate-Containing Natural Products in Diverse Bacteria

Autores: Yi-Ming Shi Jan J Crames Laura Czech Kenan A J Bozhüyük Yan-Ni Shi Merle Hirschmann Stefanie Lamberth Peter Claus Nicole Paczia Christian Rückert Jörn Kalinowski Gert Bange Helge B Bode
Publicado en: Angew Chem Int Ed, Edición 14337851, 2022, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202206106

Activation, Structure, Biosynthesis and Bioactivity of Glidobactin‐like Proteasome Inhibitors from Photorhabdus laumondii

Autores: Lei Zhao, Camille Le Chapelain, Alexander O. Brachmann, Marcel Kaiser, Michael Groll, Helge B. Bode
Publicado en: ChemBioChem, Edición 22/9, 2021, Página(s) 1582-1588, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202100014

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Autores: Andreas Tietze, Yan‐Ni Shi, Max Kronenwerth, Helge B. Bode
Publicado en: ChemBioChem, Edición 21/19, 2020, Página(s) 2750-2754, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000176

Integrating genomics and metabolomics for scalable non-ribosomal peptide discovery

Autores: Bahar Behsaz, Edna Bode, Alexey Gurevich, Yan-Ni Shi, Florian Grundmann, Deepa Acharya, Andrés Mauricio Caraballo-Rodríguez, Amina Bouslimani, Morgan Panitchpakdi, Annabell Linck, Changhui Guan, Julia Oh, Pieter C. Dorrestein, Helge B. Bode, Pavel A. Pevzner, Hosein Mohimani
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-23502-4

Microbial Cationic Peptides as a Natural Defense Mechanism against Insect Antimicrobial Peptides

Autores: Tien Duy Vo, Christoph Spahn, Mike Heilemann, Helge B. Bode
Publicado en: ACS Chemical Biology, Edición 16/3, 2021, Página(s) 447-451, ISSN 1554-8929
Editor: American Chemical Society
DOI: 10.1021/acschembio.0c00794

Non-peer reviewed articles (4)

Influence of condensation domains on activity and specificity of adenylation domains

Autores: Janik Kranz, Sebastian L. Wenski, Alexander A. Dichter, Helge B. Bode, Kenan A. J. Bozhüyük
Publicado en: bioRxiv, 2021, ISSN 2692-8205
Editor: Cold Spring Harbor
DOI: 10.1101/2021.08.23.457306

Polyketide synthase-derived sphingolipids determine microbiota-mediated protection against pathogens in C. elegans

Autores: Lena Peters, Moritz Drechsler, Barbara Pees, Georgia Angelidou, Liesa Salzer, Karlis Arturs Moors, Nicole Paczia, Hinrich Schulenburg, Yi-Ming Shi, Christoph Kaleta, Michael Witting, Helge B. Bode, Katja Dierking
Publicado en: bioRxiv, 2024, ISSN 0362-4331
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.02.06.579051

Synthetische Zipper für die Biosynthese neuer Naturstoffderivate

Autores: Nadya Abbood Kenan A.J. Bozhüyük Helge B. Bode
Publicado en: Biospektrum, Edición 09470867, 2022, Página(s) 446-449, ISSN 0947-0867
Editor: Spektrum Akademischer Verlag GmbH
DOI: 10.1007/s12268-022-1785-7

The Chemical Structure of Widespread Microbial Aryl Polyene Lipids

Autores: Gina L. C. Grammbitter, Yi-Ming Shi, Yan-Ni Shi, Sahithya P. B. Vemulapalli, Christian Richter, Harald Schwalbe, Mohammad Alanjary, Anja Schüffler, Matthias Witt, Christian Griesinger, Helge B. Bode
Publicado en: bioRxiv, 2020, ISSN 2692-8205
Editor: Cold Spring Harbor
DOI: 10.1101/2020.12.19.423268

Derechos de propiedad intelectual

Patent (1)

ADAPTER SYSTEM OF ARTIFICIAL NON-RIBOSOMAL PEPTIDE SYNTHETASES AND POLYKETIDE SYNTHASES

Número de solicitud/publicación: 19 208603
Fecha: 2019-11-12
Solicitante(s): JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN