Synthetic biology of non-ribosomal peptide synthetases to generate new peptides

Informazioni relative al progetto

SYNPEP

ID dell’accordo di sovvenzione: 835108

DOI 10.3030/835108

Data della firma CE 30 Luglio 2019

Data di avvio 1 Ottobre 2019

Data di completamento 30 Settembre 2024

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale € 3 165 788,00

Contributo UE € 3 165 788,00

Coordinato da

JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Germany

Pubblicazioni

Peer reviewed articles (20)

Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria

Autori: Yi-Ming Shi Merle Hirschmann Yan-Ni Shi Shabbir Ahmed Desalegne Abebew, Nicholas J. Tobias, Peter Grün, Jan J. Crames, Laura Pöschel, Wolfgang Kuttenlochner, Christian Richter, Jennifer Herrmann, Rolf Müller, Aunchalee Thanwisai, Sacha J. Pidot, Timothy P. Stinear, Michael Groll, Yonggyun Kim Helge B. Bode
Pubblicato in: Nature Chemistry, 2022, ISSN 1755-4330
Editore: Nature Publishing Group
DOI: 10.1038/s41557-022-00923-2

Evolution-inspired engineering of nonribosomal peptide synthetases

Autori: Kenan A. J. Bozhueyuek, Leonard Präve, Carsten Kegler, Leonie Schenk, Sebastian Kaiser, Christian Schelhas, Yan-Ni Shi, Wolfgang Kuttenlochner, Max Schreiber, Joshua Kandler, Mohammad Alanjary, T.M. Mohiuddin, Michael Groll, Georg K. A. Hochberg, Helge B. Bode
Pubblicato in: Science, Numero 383 (6689), 2024, Pagina/e eadg4320, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.adg4320

A Practical Guideline to Engineering Nonribosomal Peptide Synthetases

Autori: Nadya Abbood, Leonard Praeve, Kenan Bozhüyük, Helge B. Bode
Pubblicato in: Methods in Molecular Biology (MIMB), Numero 2670, 2023, Pagina/e 219-234, ISSN 1064-3745
Editore: Humana Press, Inc.
DOI: 10.1007/978-1-0716-3214-7

NMR resonance assignments for a docking domain pair with an attached thiolation domain from the PAX peptide-producing NRPS from Xenorhabdus cabanillasii

Autori: Jonas Watzel, Sepas Sarawi, Elke Duchardt-Ferner, Helge B. Bode, Jens Wöhnert
Pubblicato in: Biomolecular NMR Assignments, Numero 15/1, 2021, Pagina/e 229-234, ISSN 1874-2718
Editore: Springer Verlag
DOI: 10.1007/s12104-021-10010-1

Type S Non-Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries

Autori: Nadya Abbood Tien Duy Vo Jonas Watzel Kenan A J Bozhueyuek Helge B Bode
Pubblicato in: Chem Eur J, Numero 09476539, 2022, Pagina/e e202103963, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202103963

Nematode-Associated Bacteria: Production of Antimicrobial Agent as a Presumptive Nominee for Curing Endodontic Infections Caused by Enterococcus faecalis

Autori: Hicran Donmez Ozkan, Harun Cimen, Derya Ulug, Sebastian Wenski, Senem Yigit Ozer, Murat Telli, Neriman Aydin, Helge B. Bode, Selcuk Hazir
Pubblicato in: Frontiers in Microbiology, Numero 10, 2019, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2019.02672

Cooperation between a T Domain and a Minimal C‐Terminal Docking Domain to Enable Specific Assembly in a Multiprotein NRPS

Autori: Jonas Watzel, Elke Duchardt‐Ferner, Sepas Sarawi, Helge B. Bode, Jens Wöhnert
Pubblicato in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202103498

Structure and biosynthesis of deoxy-polyamine in Xenorhabdus bovienii

Autori: Sebastian L Wenski Natalie Berghaus Nadine Keller Helge B Bode
Pubblicato in: J Ind Microbiol Biotechnol, Numero 13675435, 2021, Pagina/e kuab006, ISSN 1367-5435
Editore: Springer Verlag
DOI: 10.1093/jimb/kuab006

A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments

Autori: Eva M. Huber, Lukas Kreling, Antje K. Heinrich, Maximilian Dünnebacke, Alexander Pöthig, Helge B. Bode, Michael Groll
Pubblicato in: Structure, Numero 31 (5), 2023, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2023.03.001

easyPACId, a Simple Method for Induced Production, Isolation, Identification, and Testing of Natural Products from Proteobacteria

Autori: Edna Bode, Daniela Assmann, Petra Happel, Elmar Meyer, Karin Münch, Nicole Rössel, Helge B. Bode
Pubblicato in: bio-protocol, Numero 13(13): e4709, 2023, ISSN 2331-8325
Editore: Bio-Protocol LLC
DOI: 10.21769/bioprotoc.4709

Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Autori: Alexander Rill, Lei Zhao, Helge B. Bode
Pubblicato in: Microbial Cell Factories, Numero 23 (1), 2024, Pagina/e 98, ISSN 1475-2859
Editore: BioMed Central
DOI: 10.1186/s12934-024-02363-8

Engineering of Specific Single-Module Nonribosomal Peptide Synthetases of the RXP Type for the Production of Defined Peptides

Autori: Xiaofeng Cai, Lei Zhao, Helge B. Bode
Pubblicato in: ACS Synthetic Biology, Numero 12 (1), 2023, Pagina/e 203-212, ISSN 2161-5063
Editore: American Chemical Society
DOI: 10.1021/acssynbio.2c00472

Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii involves cross-talk between specialized and primary metabolism

Autori: Siyar Kavakli, Gina L.C.Grammbitter, Helge B.Bode
Pubblicato in: Tetrahedron, 2022, ISSN 1464-5416
Editore: Elsevier
DOI: 10.1016/j.tet.2022.133116

Cleavage Off-Loading and Post-assembly-Line Conversions Yield Products with Unusual Termini during Biosynthesis

Autori: Yi-Ming Shi Merle Hirschmann Yan-Ni Shi Helge B Bode
Pubblicato in: ACS Chem Biol, Numero 15548929, 2022, Pagina/e 2221-2228, ISSN 1554-8929
Editore: American Chemical Society
DOI: 10.1021/acschembio.2c00367

Synthetic Zippers as an Enabling Tool for Engineering of Non-Ribosomal Peptide Synthetases

Autori: Kenan A. J. Bozhueyuek; Jonas Watzel; Nadya Abbood; Helge B. Bode
Pubblicato in: Angew Chem Int Ed, Numero 1, 2021, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1101/2020.05.06.080655

Genome Mining Enabled by Biosynthetic Characterization Uncovers a Class of Benzoxazolinate-Containing Natural Products in Diverse Bacteria

Autori: Yi-Ming Shi Jan J Crames Laura Czech Kenan A J Bozhüyük Yan-Ni Shi Merle Hirschmann Stefanie Lamberth Peter Claus Nicole Paczia Christian Rückert Jörn Kalinowski Gert Bange Helge B Bode
Pubblicato in: Angew Chem Int Ed, Numero 14337851, 2022, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202206106

Activation, Structure, Biosynthesis and Bioactivity of Glidobactin‐like Proteasome Inhibitors from Photorhabdus laumondii

Autori: Lei Zhao, Camille Le Chapelain, Alexander O. Brachmann, Marcel Kaiser, Michael Groll, Helge B. Bode
Pubblicato in: ChemBioChem, Numero 22/9, 2021, Pagina/e 1582-1588, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202100014

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Autori: Andreas Tietze, Yan‐Ni Shi, Max Kronenwerth, Helge B. Bode
Pubblicato in: ChemBioChem, Numero 21/19, 2020, Pagina/e 2750-2754, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000176

Integrating genomics and metabolomics for scalable non-ribosomal peptide discovery

Autori: Bahar Behsaz, Edna Bode, Alexey Gurevich, Yan-Ni Shi, Florian Grundmann, Deepa Acharya, Andrés Mauricio Caraballo-Rodríguez, Amina Bouslimani, Morgan Panitchpakdi, Annabell Linck, Changhui Guan, Julia Oh, Pieter C. Dorrestein, Helge B. Bode, Pavel A. Pevzner, Hosein Mohimani
Pubblicato in: Nature Communications, Numero 12/1, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-23502-4

Microbial Cationic Peptides as a Natural Defense Mechanism against Insect Antimicrobial Peptides

Autori: Tien Duy Vo, Christoph Spahn, Mike Heilemann, Helge B. Bode
Pubblicato in: ACS Chemical Biology, Numero 16/3, 2021, Pagina/e 447-451, ISSN 1554-8929
Editore: American Chemical Society
DOI: 10.1021/acschembio.0c00794

Non-peer reviewed articles (3)

Influence of condensation domains on activity and specificity of adenylation domains

Autori: Janik Kranz, Sebastian L. Wenski, Alexander A. Dichter, Helge B. Bode, Kenan A. J. Bozhüyük
Pubblicato in: bioRxiv, 2021, ISSN 2692-8205
Editore: Cold Spring Harbor
DOI: 10.1101/2021.08.23.457306

Synthetische Zipper für die Biosynthese neuer Naturstoffderivate

Autori: Nadya Abbood Kenan A.J. Bozhüyük Helge B. Bode
Pubblicato in: Biospektrum, Numero 09470867, 2022, Pagina/e 446-449, ISSN 0947-0867
Editore: Spektrum Akademischer Verlag GmbH
DOI: 10.1007/s12268-022-1785-7

The Chemical Structure of Widespread Microbial Aryl Polyene Lipids

Autori: Gina L. C. Grammbitter, Yi-Ming Shi, Yan-Ni Shi, Sahithya P. B. Vemulapalli, Christian Richter, Harald Schwalbe, Mohammad Alanjary, Anja Schüffler, Matthias Witt, Christian Griesinger, Helge B. Bode
Pubblicato in: bioRxiv, 2020, ISSN 2692-8205
Editore: Cold Spring Harbor
DOI: 10.1101/2020.12.19.423268

Diritti di proprietà intellettuale

Patent (1)

ADAPTER SYSTEM OF ARTIFICIAL NON-RIBOSOMAL PEPTIDE SYNTHETASES AND POLYKETIDE SYNTHASES

Numero candidatura/pubblicazione: 19 208603
Data: 2019-11-12