Projektbeschreibung
Entschlüsselung des therapeutischen Potenzials von Fruchtwasser-Stammzellen
Fruchtwasser, das für seine trophische und unterstützende Rolle in der Embryonalentwicklung bekannt ist, enthält eine kleine Population von Zellen, die als Fruchtwasser-Stammzellen bezeichnet werden. Obwohl diese Zellen für die pränatale Diagnostik genutzt werden, ist wenig über ihre Entstehung und Rolle bekannt. Im Rahmen des EU-finanzierten Projekts AmnioticID soll die Abstammung dieser Zellen und deren Rolle in der Entwicklung mittels Sequenzierung der nächsten Generation und in vivo-Ansätzen zur Abstammungsverfolgung bestimmt werden. Die Ergebnisse werden den Weg für die Isolierung und Expansion dieser Zellen für regenerative medizinische Konzepte ebnen.
Ziel
The amniotic fluid surrounds and protects the foetus during development, providing trophic and mechanical support to its growth. This liquid contains a variety of cells shedding from embryonic and extra-embryonic tissues that have long been utilised for prenatal diagnosis. In a seminal 2007 article, Professor De Coppi identified in the amniotic fluid, a small population of cells with broad multi-lineage differentiation ability defined as Amniotic Fluid Stem Cells (AFSCs). AFSCs can be isolated, expanded and differentiated during gestation, making them ideal for the development of pre-/perinatal autologous regenerative medicine strategies. In the last decade, a series of studies focused on investigating the therapeutic relevance of the AFSCs for various tissue compartments, such as skeletal and cardiac muscle, lung and liver. However, very little is known about the AFSCs origin, lineage identity and role during human development. This knowledge gaps have relevant impact on these cells' clinical translation and a list of important questions remains to be addressed: Which tissue releases the AFSCs? Are these cells constantly generated, or part of a self-renewing niche? What is the function of the AFSCs during human development? This proposal aims at addressing these questions, by using a combination of next generation sequencing analyses, cell biology assays and in vivo linage tracing approaches. Ultimately defining the origin and identity of the AFSCs will not only increase our knowledge of their physiological role, but will also allow to better direct future studies on their therapeutic implications. Thanks to this project, I will gain a comprehensive, multi-faceted understanding of the biology and regenerative potential of AFSCs. This will open up new research agendas, foster international collaborations and provide me valuable transferrable skills of crucial relevance to develop my independent career, in line with the mission of the Marie Curie action.
Wissenschaftliches Gebiet
Not validated
Not validated
Schlüsselbegriffe
Programm/Programme
Thema/Themen
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenFinanzierungsplan
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordinator
WC1E 6BT London
Vereinigtes Königreich