Periodic Reporting for period 2 - BRILLIANCE (Bright, coherent and focused light to resolve neural circuits)
Periodo di rendicontazione: 2021-09-01 al 2023-02-28
We developed and implemented a large sensor, high resolution indirect X-ray imaging detector to accelerate data collection in holographic nano-tomography.
We obtained evidence that 3D X-ray microscopy is capable of resolving synapses in heavy metal stained, resin embedded brain tissue samples.
With deep learning based self-supervised image restoration we could improve the spatial resolution and accelerate data acquisition in coherent X-ray microscopy.
We generated unprecedented data sets of circuit modules in mouse brain and of multiple full brains in a small model organism to investigate variability between healthy individuals and the architectural changes associated to neurological diseases.
We generated unique data that enables addressing new scientific questions both related to fundamental understanding of brain function and to finding ways to tackle neurological diseases.