PHAGOSCOPY: Dissecting cell-autonomous immunity with ex vivo electron cryo-microscopy

Project Information

PHAGOSCOPY

Grant agreement ID: 852880

DOI

10.3030/852880

Project closed

EC signature date 5 November 2019

Start date 1 February 2020

End date 31 January 2026

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 438 510,00

EU contribution

€ 1 438 510,00

Coordinated by

TECHNISCHE UNIVERSITEIT DELFT
Netherlands

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Open Research Data Pilot (1)

Data Management Plan

A data management plan on how data generated by the project are stored and made available

Publications

Other (2)

Interactive segmentation of membrane and membrane mimic densities in cryo-EM maps

Author(s): Alok Bharadwaj, Lotte Veerbeek, Arjen J. Jakobi
Published in: BioRxiv, 2026
Publisher: openRxiv
DOI: 10.64898/2026.02.25.707988

Confidence-guided cryo-EM map optimisation with LocScale-2.0

Author(s): Alok Bharadwaj, Reinier de Bruin, Arjen J. Jakobi
Published in: BioRxiv, 2026
Publisher: openRxiv
DOI: 10.1101/2025.09.11.674726

Peer reviewed articles (9)

Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes

Author(s): Stefan T. Huber; Edin Sarajlic; Roeland Huijink; Felix Weis; Wiel H. Evers; Arjen J. Jakobi
Published in: eLife, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications

Robust Local Thickness Estimation of Sub‐Micrometer Specimen by 4D‐STEM

Author(s): Radim Skoupý, Daan B. Boltje, Miroslav Slouf, Kateřina Mrázová, Tomáš Láznička, Clémence M. Taisne, Vladislav Krzyžánek, Jacob P. Hoogenboom, Arjen J. Jakobi
Published in: Small Methods, Issue 7, 2024, ISSN 2366-9608
Publisher: Wiley-VCH GmbH
DOI: 10.1002/smtd.202300258

Thickness- and quality-controlled fabrication of fluorescence-targeted frozen-hydrated lamellae

Author(s): Daan B. Boltje, Radim Skoupý, Clémence Taisne, Wiel H. Evers, Arjen J. Jakobi, Jacob P. Hoogenboom
Published in: Cell Reports Methods, Issue 5, 2025, Page(s) 101004, ISSN 2667-2375
Publisher: Cell Press
DOI: 10.1016/j.crmeth.2025.101004

Structural basis of antimicrobial membrane coat assembly by human GBP1

Author(s): Tanja Kuhm, Clémence Taisne, Cecilia de Agrela Pinto, Luca Gross, Evdokia A. Giannopoulou, Stefan T. Huber, Els Pardon, Jan Steyaert, Sander J. Tans, Arjen J. Jakobi
Published in: Nature Structural & Molecular Biology, Issue 32, 2025, Page(s) 172-184, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-024-01400-9

Workflow for Fluorescence-Targeted Lamella Milling From Vitrified Cells With a Coincident Fluorescence, Electron, and Ion Beam Microscope

Author(s): Elise Perton, Daan Boltje, Arjen Jakobi, Jacob Hoogenboom
Published in: BIO-PROTOCOL, Issue 15, 2025, ISSN 2331-8325
Publisher: Bio-Protocol, LLC
DOI: 10.21769/bioprotoc.5390

Structural biology of microbial gas vesicles: historical milestones and current knowledge

Author(s): Stefan T. Huber, Arjen J. Jakobi
Published in: Biochemical Society Transactions, Issue 52, 2024, Page(s) 205-215, ISSN 0300-5127
Publisher: Portland Press, Ltd.
DOI: 10.1042/bst20230396

A cryogenic, coincident fluorescence, electron, and ion beam microscope

Author(s): Daan B Boltje, Jacob P Hoogenboom, Arjen J Jakobi, Grant J Jensen, Caspar TH Jonker, Max J Kaag, Abraham J Koster, Mart GF Last, Cecilia de Agrela Pinto, Jürgen M Plitzko, Stefan Raunser, Sebastian Tacke, Zhexin Wang, Ernest B van der Wee, Roger Wepf, Sander den Hoedt
Published in: eLife, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.82891

Femtolitre Volume cryoEM Sample Preparation Using a Force‐Sensitive Microfluidic Cantilever

Author(s): Vijayendra Shastri, Joachim Pronk, Eleonoor Verlinden, Daniel Torres González, Edin Sarajlic, Paul Laeven, Wiel Evers, Patrick Frederix, Urs Staufer, Arjen J. Jakobi, Murali Krishna Ghatkesar, Andreas Engel
Published in: Advanced Materials Technologies, Issue 10, 2026, ISSN 2365-709X
Publisher: Wiley
DOI: 10.1002/admt.202501333

Electron scattering properties of biological macromolecules and their use for cryo-EM map sharpening

Author(s): Arjen Jakobi; Alok Bharadwaj
Published in: Faraday Discussions, 2022, Page(s) 168-183, ISSN 1359-6640
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2fd00078d