Gene Therapy to restore lymphatic flow lymphedema

Project Information

TheraLymph

Grant agreement ID: 874708

DOI

10.3030/874708

Project closed

EC signature date 24 November 2019

Start date 1 January 2020

End date 30 June 2025

Funded under

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Total cost

€ 8 064 946,00

EU contribution

€ 8 064 946,00

Coordinated by

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Open Research Data Pilot (1)

Data Management Plan

Demonstrators, pilots, prototypes (2)

Intralymphatic injection of Luciferase expressing lentivector in mice
Design and validate probes for CRISPR ON, CRISPR/CAS9, CRISPR/CAS13

Documents, reports (1)

Intralymphatic injection of GFP expressing lentivector in mice

Websites, patent fillings, videos etc. (1)

Project website

Publications

Thesis and dissertations (1)

Functional Crosstalk between Human Adipose Tissue and Lymphatic System

Author(s): Zuzana Varaliová
Published in: 2022
Publisher: Univerzita Karlova

Peer reviewed articles (39)

Lymphangiogenesis requires Ang2/Tie/PI3K signaling for VEGFR3 cell-surface expression

Author(s): Emilia A. Korhonen, Aino Murtomäki, Sawan Kumar Jha, Andrey Anisimov, Anne Pink, Yan Zhang, Simon Stritt, Inam Liaqat, Lukas Stanczuk, Laura Alderfer, Zhiliang Sun, Emmi Kapiainen, Abhishek Singh, Ibrahim Sultan, Anni Lantta, Veli-Matti Leppänen, Lauri Eklund, Yulong He, Hellmut G. Augustin, Kari Vaahtomeri, Pipsa Saharinen, Taija Mäkinen, and Kari Alitalo
Published in: The Journal of Clinical Investigation, Issue 132(15), 2022, Page(s) e155478, ISSN 1558-8238
Publisher: ASCI
DOI: 10.1172/jci155478

Lymphedema alters lipolytic, lipogenic, immune and angiogenic properties of adipose tissue: a hypothesis-generating study in breast cancer survivors

Author(s): Michal Koc, Martin Wald, Zuzana Varaliová, Barbora Ondrůjová, Terezie Čížková, Milan Brychta, Jana Kračmerová, Lenka Beranová, Jan Pala, Veronika Šrámková, Michaela Šiklová, Jan Gojda, Lenka Rossmeislová
Published in: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-87494-3

The Impact of Estrogen Receptor in Arterial and Lymphatic Vascular Diseases

Author(s): Coralie Fontaine, Florent Morfoisse, Florence Tatin, Audrey Zamora, Rana Zahreddine, Daniel Henrion, Jean-François Arnal, Françoise Lenfant, Barbara Garmy-Susini
Published in: International Journal of Molecular Sciences, Issue 21/9, 2020, Page(s) 3244, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21093244

Transport functions of intestinal lymphatic vessels

Author(s): Patrick Tso, Jeremiah Bernier-Latmani, Tatiana V. Petrova, Min Liu
Published in: Nature Reviews Gastroenterology & Hepatology, Issue 22, 2025, Page(s) 127-145, ISSN 1759-5045
Publisher: Nature Publishing Group
DOI: 10.1038/s41575-024-00996-z

Epilepsy with faint capillary malformation or reticulated telangiectasia associated with mosaic <i>AKT3</i> pathogenic variants

Author(s): Martina De Bortoli, Marta Ivars, Nicole Revencu, Marie‐Cécile Nassogne, Cinzia Lavarino, Sonia Paco, Martin Lammens, Anne Renders, Dana Dumitriu, Raphaël Helaers, Laurence M. Boon, Eulalia Baselga, Miikka Vikkula
Published in: American Journal of Medical Genetics Part A, Issue 194, 2024, ISSN 1552-4825
Publisher: Wiley-Liss Inc
DOI: 10.1002/ajmg.a.63551

Mechanosensitive mTORC1 signaling maintains lymphatic valves

Author(s): Cansaran Saygili Demir, Amélie Sabine, Muyun Gong, Olivier Dormond, Tatiana V. Petrova
Published in: Journal of Cell Biology, Issue 222, 2023, ISSN 0021-9525
Publisher: Rockefeller University Press
DOI: 10.1083/jcb.202207049

VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells

Author(s): Manon Penco-Campillo, Yannick Comoglio, Álvaro Javier Feliz Morel, Rita Hanna, Jérôme Durivault, Magalie Leloire, Bastien Mejias, Marina Pagnuzzi, Amandine Morot, Fanny Burel-Vandenbos, Matthew Selby, Daniel Williamson, Steven C. Clifford, Audrey Claren, Jérôme Doyen, Vincent Picco, Sonia Martial, Gilles Pagès
Published in: Communications Biology, Issue 3/1, 2020, ISSN 2399-3642
Publisher: Nature publishing group
DOI: 10.1038/s42003-020-01306-4

Comparative impact of proton versus photon irradiation on triple‐negative breast cancer: Role of VEGFC in tumour aggressiveness

Author(s): Saharnaz Sarlak, Delphine Marotte, Arthur Karaulic, Jessy Sirera, Alessandra Pierantoni, Meng‐Chen Tsai, Roxane Sylvestre, Clement Molina, Arthur Gouraud, Aurélien Bancaud, Paraskevi Kousteridou, Marie Vidal, Joël Hérault, Jérôme Doyen, Maeva Dufies, Florent Morfoisse, Barbara Garmy‐Susini, Frédéric Luciano, Gilles Pagès
Published in: Clinical and Translational Medicine, Issue 15, 2025, ISSN 2001-1326
Publisher: Wiley
DOI: 10.1002/ctm2.70330

Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema

Author(s): Justine Creff, Asalaa Lamaa, Emeline Benuzzi, Elisa Balzan, Francoise Pujol, Tangra Draia-Nicolau, Manon Nougué, Lena Verdu, Florent Morfoisse, Eric Lacazette, Philippe Valet, Benoit Chaput, Fabian Gross, Regis Gayon, Pascale Bouillé, Julie Malloizel-Delaunay, Alessandra Bura-Rivière, Anne-Catherine Prats, Barbara Garmy-Susini
Published in: EMBO Molecular Medicine, Issue 16, 2024, Page(s) 386-415, ISSN 1757-4684
Publisher: EMBO Press
DOI: 10.1038/s44321-023-00017-7

Lymphatic malformations: mechanistic insights and evolving therapeutic frontiers

Author(s): Milena Petkova, Ingvar Ferby, Taija Mäkinen
Published in: Journal of Clinical Investigation, Issue 134, 2024, ISSN 1558-8238
Publisher: Amercian Society for Clinical Investigtaion
DOI: 10.1172/jci172844

Single and combined impacts of irradiation and surgery on lymphatic vasculature and fibrosis associated to secondary lymphedema

Author(s): F. Buntinx, A. Lebeau, L. Gillot, L. Baudin, R. Ndong Penda, F. Morfoisse, F. Lallemand, G. Vottero, C. Nizet, J. L. Nizet, S. Blacher, A. Noel
Published in: Frontiers in Pharmacology, Issue 13, 2022, ISSN 1663-9812
Publisher: Frontiers Media S.A.
DOI: 10.3389/fphar.2022.1016138

Ureteropelvic junction obstruction with primary lymphoedema associated with<i>CELSR1</i>variants

Author(s): Murat Alpaslan, Sandrine Mestré-Godin, Aurélie Lay, Guido Giacalone, Raphaël Helaers, Salma Adham, Hélène Kovacsik, Sophie Guillemard, Erick Mercier, Laurence Boon, Nicole Revencu, Pascal Brouillard, Isabelle Quere, Miikka Vikkula
Published in: Journal of Medical Genetics, Issue 60, 2023, Page(s) 1161-1168, ISSN 0022-2593
Publisher: British Medical Association
DOI: 10.1136/jmg-2023-109171

Contribution of VEGF-B-Induced Endocardial Endothelial Cell Lineage in Physiological Versus Pathological Cardiac Hypertrophy

Author(s): Ibrahim Sultan, Markus Ramste, Pim Peletier, Karthik Amudhala Hemanthakumar, Deepak Ramanujam, Annakaisa Tirronen, Ylva von Wright, Salli Antila, Pipsa Saharinen, Lauri Eklund, Eero Mervaala, Seppo Ylä-Herttuala, Stefan Engelhardt, Riikka Kivelä, Kari Alitalo
Published in: Circulation Research, Issue 134, 2024, Page(s) 1465-1482, ISSN 0009-7330
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/circresaha.123.324136

Cardiac Lymphangiogenesis in CVDs

Author(s): Ebba Brakenhielm, Ibrahim Sultan, Kari Alitalo
Published in: Arteriosclerosis, Thrombosis, and Vascular Biology, Issue 44, 2024, Page(s) 1016-1020, ISSN 1079-5642
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/atvbaha.123.319572

Targeted treatment in complex lymphatic anomaly: a case of synergistic efficacy of trametinib and sirolimus

Author(s): Emmanuel Seront, Antoine Froidure, Nicole Revencu, Valerie Dekeuleneer, Philippe Clapuyt, Dana Dumitriu, Miikka Vikkula, Laurence M. Boon
Published in: Orphanet Journal of Rare Diseases, Issue 19, 2024, ISSN 1750-1172
Publisher: BioMed Central
DOI: 10.1186/s13023-024-03211-z

Pathogenic variants in <i>HGF</i> give rise to childhood-to-late onset primary lymphoedema by loss of function

Author(s): Murat Alpaslan, Elodie Fastré, Sandrine Mestre, Arie van Haeringen, Gabriela M Repetto, Kathelijn Keymolen, Laurence M Boon, Florence Belva, Guido Giacalone, Nicole Revencu, Yves Sznajer, Katie Riches, Vaughan Keeley, Sahar Mansour, Kristiana Gordon, Silvia Martin-Almedina, Sara Dobbins, Pia Ostergaard, Isabelle Quere, Pascal Brouillard, Miikka Vikkula
Published in: Human Molecular Genetics, Issue 33, 2024, Page(s) 1250-1261, ISSN 0964-6906
Publisher: Oxford University Press
DOI: 10.1093/hmg/ddae060

Development and aging of the lymphatic vascular system

Author(s): Alejandra González-Loyola, Tatiana V. Petrova
Published in: Advanced Drug Delivery Reviews, Issue 169, 2021, Page(s) 63-78, ISSN 0169-409X
Publisher: Elsevier BV
DOI: 10.1016/j.addr.2020.12.005

Angiogenesis and Lymphangiogenesis in Medulloblastoma Development

Author(s): Manon Penco-Campillo, Gilles Pages, Sonia Martial
Published in: Biology, Issue 12, 2023, Page(s) 1028, ISSN 2079-7737
Publisher: MDPI
DOI: 10.3390/biology12071028

Lymphatic Malformations: Genetics, Mechanisms and Therapeutic Strategies

Author(s): Taija Mäkinen, Laurence M. Boon, Miikka Vikkula, Kari Alitalo
Published in: Circulation Research, Issue 129/1, 2021, Page(s) 136-154, ISSN 0009-7330
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/circresaha.121.318142

Preliminary results of the European multicentric phase III trial regarding sirolimus in slow-flow vascular malformations

Author(s): Emmanuel Seront, An Van Damme, Catherine Legrand, Annouk Bisdorff-Bresson, Philippe Orcel, Thomas Funck-Brentano, Marie-Antoinette Sevestre, Anne Dompmartin, Isabelle Quere, Pascal Brouillard, Nicole Revencu, Martina De Bortoli, Frank Hammer, Philippe Clapuyt, Dana Dumitriu, Miikka Vikkula, Laurence M. Boon
Published in: JCI Insight, Issue 8, 2023, ISSN 2379-3708
Publisher: American Society for Clinical Investigation
DOI: 10.1172/jci.insight.173095

Coordinating Effect of VEGFC and Oleic Acid Participates to Tumor Lymphangiogenesis

Author(s): Florent Morfoisse, Fabienne De Toni, Jeremy Nigri, Mohsen Hosseini, Audrey Zamora, Florence Tatin, Françoise Pujol, Jean-Emmanuel Sarry, Dominique Langin, Eric Lacazette, Anne-Catherine Prats, Richard Tomasini, Jean Galitzky, Anne Bouloumié, Barbara Garmy-Susini
Published in: Cancers, Issue 13/12, 2021, Page(s) 2851, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13122851

Yoda1 opens the lymphatic path for craniosynostosis therapy

Author(s): Aleksanteri Aspelund, Kari Alitalo
Published in: Journal of Clinical Investigation, Issue 134, 2024, ISSN 1558-8238
Publisher: American Society for Clinical Investigation
DOI: 10.1172/jci176858

Molecular landscape and classification of vascular anomalies

Author(s): Emmanuel Seront, Angela Queisser, Laurence M. Boon, Miikka Vikkula
Published in: Hematology, Issue 2024, 2024, Page(s) 700-708, ISSN 1520-4391
Publisher: American Society of Hematology
DOI: 10.1182/hematology.2024000598

Somatic Loss-of-Function PIK3R1 and Activating Non-hotspot PIK3CA Mutations Associated with Capillary Malformation with Dilated Veins (CMDV)

Author(s): Martina De Bortoli, Angela Queisser, Van Cuong Pham, Anne Dompmartin, Raphaël Helaers, Simon Boutry, Cathy Claus, An-Katrien De Roo, Frank Hammer, Pascal Brouillard, Salim Abdelilah-Seyfried, Laurence M. Boon, Miikka Vikkula
Published in: Journal of Investigative Dermatology, Issue 144, 2024, Page(s) 2066-2077.e6, ISSN 0022-202X
Publisher: Blackwell Publishing Inc.
DOI: 10.1016/j.jid.2024.01.033

A case report of sirolimus use in early fetal management of lymphatic malformation

Author(s): Emmanuel Seront, Jean Marc Biard, An Van Damme, Nicole Revencu, Benoit Lengelé, Sandra Schmitz, Caroline de Toeuf, Philippe Clapuyt, Francis Veyckemans, Caroline Prégardien, Miikka Vikkula, Pierre Bernard, Laurence M. Boon
Published in: Nature Cardiovascular Research, Issue 2, 2023, Page(s) 595-599, ISSN 2731-0590
Publisher: Springer Nature
DOI: 10.1038/s44161-023-00280-4

Mechanisms and functions of intestinal vascular specialization

Author(s): Jeremiah Bernier-Latmani, Alejandra González-Loyola, Tatiana V. Petrova
Published in: Journal of Experimental Medicine, Issue 221, 2023, ISSN 0022-1007
Publisher: Rockefeller University Press
DOI: 10.1084/jem.20222008

Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance

Author(s): Sinem Karaman, Satu Paavonsalo, Krista Heinolainen, Madeleine H. Lackman, Amanda Ranta, Karthik A. Hemanthakumar, Yoshiaki Kubota, Kari Alitalo
Published in: Journal of experimental medicine, Issue March 7, 219(3à, 2022, Page(s) e20210565, ISSN 0022-1007
Publisher: Rockefeller University Press
DOI: 10.1084/jem.20210565

AXL promotes lymphangiogenesis by amplifying VEGF-C-mediated AKT pathway

Author(s): Sébastien Pirson, Marine Gautier-Isola, Louis Baudin, Loïc Rouaud, Aline Vanwynsberghe, Jonathan Deroye, Sophie Bekisz, Fabrice Gucciardo, Alizée Lebeau, Florence Buntinx, Elitsa Ivanova, Bernard Staumont, Silvia Blacher, Christine Gilles, Agnès Noël
Published in: Cellular and Molecular Life Sciences, Issue 82, 2025, ISSN 1420-9071
Publisher: Springer Science and Business Media LLC
DOI: 10.1007/s00018-024-05542-3

Enhancing radiotherapy techniques for Triple-Negative breast cancer treatment

Author(s): Saharnaz Sarlak, Gilles Pagès, Frédéric Luciano
Published in: Cancer Treatment Reviews, Issue 136, 2025, Page(s) 102939, ISSN 0305-7372
Publisher: W. B. Saunders Co., Ltd.
DOI: 10.1016/j.ctrv.2025.102939

Sex Hormones in Lymphedema

Author(s): Florent Morfoisse, Audrey Zamora, Emmanuelle Marchaud, Manon Nougue, Leila H. Diallo, Florian David, Emilie Roussel, Eric Lacazette, Anne-Catherine Prats, Florence Tatin, Barbara Garmy-Susini
Published in: Cancers, Issue 13/3, 2021, Page(s) 530, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13030530

Lessons of Vascular Specialization From Secondary Lymphoid Organ Lymphatic Endothelial Cells

Author(s): Silvia Arroz-Madeira, Tove Bekkhus, Maria H. Ulvmar, Tatiana V. Petrova
Published in: Circulation Research, Issue 132, 2024, Page(s) 1203-1225, ISSN 0009-7330
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/circresaha.123.322136

15-Lipoxygenase promotes resolution of inflammation in lymphedema by controlling Treg cell function through IFN-β

Author(s): A. Zamora, M. Nougué, L. Verdu, E. Balzan, T. Draia-Nicolau, E. Benuzzi, F. Pujol, V. Baillif, E. Lacazette, F. Morfoisse, J. Galitzky, A. Bouloumié, M. Dubourdeau, B. Chaput, N. Fazilleau, J. Malloizel-Delaunay, A. Bura-Rivière, A. C. Prats, B. Garmy-Susini
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-43554-y

Targeting uPARAP Modifies Lymphatic Vessel Architecture and Attenuates Lymphedema

Author(s): Fabrice Gucciardo, Alizée Lebeau, Sébastien Pirson, Florence Buntinx, Elitsa Ivanova, Silvia Blacher, Pascal Brouillard, Jonathan Deroye, Louis Baudin, Alexandra Pirnay, Florent Morfoisse, Claire Villette, Christophe Nizet, François Lallemand, Carine Munaut, Kari Alitalo, Liesbet Geris, Miikka Vikkula, Marine Gautier-Isola, Agnès Noel
Published in: Circulation, Issue 151, 2025, Page(s) 1412-1429, ISSN 0009-7322
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1161/circulationaha.124.072093

Translational control by long non-coding RNAs

Author(s): Anne-Claire Godet, Emilie Roussel, Nathalie Laugero, Florent Morfoisse, Eric Lacazette, Barbara Garmy-Susini, Anne-Catherine Prats
Published in: Biochimie, Issue 217, 2024, Page(s) 42-53, ISSN 0300-9084
Publisher: Elsevier BV
DOI: 10.1016/j.biochi.2023.08.015

Loss-of-function mutations of the TIE1 receptor tyrosine kinase cause late-onset primary lymphedema

Author(s): Pascal Brouillard, Aino Murtomäki, Veli-Matti Leppänen, Marko Hyytiäinen, Sandrine Mestre, Lucas Potier, Laurence M. Boon, Nicole Revencu, Arin Greene, Andrey Anisimov, Miia H. Salo, Reetta Hinttala, Lauri Eklund, Isabelle Quéré, Kari Alitalo, Miikka Vikkula
Published in: Journal of Clinical Investigation, Issue 134, 2024, ISSN 1558-8238
Publisher: American Society for Clinical Research
DOI: 10.1172/jci173586

Targeting of the ELR+CXCL/CXCR1/2 Pathway Is a Relevant Strategy for the Treatment of Paediatric Medulloblastomas

Author(s): Manon Penco-Campillo, Clément Molina, Patricia Piris, Nouha Soufi, Manon Carré, Marina Pagnuzzi-Boncompagni, Vincent Picco, Maeva Dufies, Cyril Ronco, Rachid Benhida, Sonia Martial, and Gilles Pagès
Published in: MDPI, Issue Volume 11 Issue 23, 2022, Page(s) 3933, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cells11233933

UPDATED HUMAN CHROMOSOME MAP OF LYMPHEDEMA-LYMPHANGIOGENESIS GENES: TEMPLATE FOR CURRENT AND FUTURE DISCOVERY

Author(s): P. Brouillard, M. Witte, R. Erickson, L. Luy, M. Vikkula
Published in: Lymphology, Issue 57, 2025, ISSN 0024-7766
Publisher: International Society of Lymphology
DOI: 10.2458/lymph.8459

Characterization of ANGPT2 mutations associated with primary lymphedema

Author(s): Veli-Matti Leppänen, Pascal Brouillard, Emilia A. Korhonen, Tuomas Sipilä, Sawan Kumar Jha, Nicole Revencu, Veerle Labarque, Elodie Fastré, Matthieu Schlögel, Marie Ravoet, Amihood Singer, Claudia Luzzatto, Donatella Angelone, Giovanni Crichiutti, Angela D’Elia, Jaakko Kuurne, Harri Elamaa, Gou Young Koh, Pipsa Saharinen, Miikka Vikkula, Kari Alitalo
Published in: Science Translational Medicine, Issue 12/560, 2020, Page(s) eaax8013, ISSN 1946-6234
Publisher: American Association for the Advancement of Science
DOI: 10.1126/scitranslmed.aax8013

Anti-Vascular Endothelial Growth Factor C Antibodies Efficiently Inhibit the Growth of Experimental Clear Cell Renal Cell Carcinomas

Author(s): Aurore Dumond, Christopher Montemagno, Valérie Vial, Renaud Grépin, Gilles Pagès
Published in: Cells, Issue 10, 2024, Page(s) 1222, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells10051222

Non-peer reviewed articles (2)

Paraxial mesoderm as a direct gateway to lymphatic endothelial cells

Author(s): Tatiana V. Petrova, Valeria V. Orlova
Published in: Nature Cardiovascular Research, Issue 4, 2025, Page(s) 11-12, ISSN 2731-0590
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s44161-024-00583-0

Lymphatic mysteries unzipped

Author(s): Tatiana V. Petrova, Gwendalyn J. Randolph
Published in: Nature Cardiovascular Research, Issue 4, 2025, Page(s) 511-513, ISSN 2731-0590
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s44161-025-00643-z

Other (1)

Aquaporin-1 sustains lymphangiogenic responses in inflammatory microenvironments

Author(s): Irena Roci, Jaeryung Kim, Kelly de Korodi, Tania Wyss, Jeremiah Bernier-Latmani, Silvia Arroz-Madeira, Esther Bovay, Hans Schoofs, Taija Mäkinen, Agnès Noël, Tatiana V. Petrova
Published in: bioRxiv preprint server, 2025
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.03.05.641652