Projektbeschreibung
Erkenntnisse zu natürlichen Killerzellen im Gewebe
Natürliche Killerzellen sind für ihre Tumorimmunität, ihre Fähigkeit, Infektionen zu bekämpfen und ihren Einfluss auf die Schwangerschaftsentwicklung bekannt. Neue Erkenntnisse aus Mausmodellen zeigen, dass im Gewebe natürliche Killerzellen vorhanden sind. Wie sie funktionieren und reguliert werden, ist jedoch unklar – vor allem beim Menschen. Das EU-finanzierte Projekt RESIDE wird nun Unterformen natürlicher Killerzellen des Menschen untersuchen, die sich permanent in der Leber und der Gebärmutter des Menschen befinden. Das Forschungsteam wird den Mechanismus untersuchen, der dafür verantwortlich ist, dass sich die Zellen im Gewebe befinden und dort rezirkulieren. Anhand eines dreidimensionalen Mikrogefäßmodells wird es außerdem die Faktoren bestimmen, welche ihre Aktivität regulieren.
Ziel
Natural killer (NK) cells contribute to tumor and antiviral immunity, regulate immune homeostasis, and impact on pregnancy success. These functions are in many instances performed in peripheral organs. Recent pioneering research in mouse models has revealed the existence and self-maintenance of tissue-resident (TR) NK cells. Although similar mechanisms are assumed to occur in humans, this has never been formally demonstrated and with our diverse infection history and active microbiota the human setting is considerably more complex. Me and my group have recently identified and characterized presumed TR NK cells in human liver and uterus. Here, we seek to challenge the prevailing concept from the mouse of how TR is formed, regulated, and maintained by taking advantage of world-unique surgical procedures for addressing lymphocyte tissue-residency, such as uterus transplantation and a novel liver surgery creating a human parabiosis situation, combined with single-cell technologies. In these settings, we will determine the detailed characteristics of subsets of NK cells permanently residing in tissues. With the different time windows as well as tissue sites presented by the clinical procedures, we will also assess kinetics of NK cell tissue repopulation. Furthermore, we aim to provide mechanistic insight into NK cell recirculation patterns by studying tissue-draining lymph fluid. Finally, we will study functional regulation of NK cell tissue-residency based on data from targeted single-cell RNAseq across multiple human tissues. The relevance of suspected and novel factors in regulating TR NK cells will be studied in a 3D microvasculature model combined with siRNA-based silencing of candidate genes. TR immune cells are poised to instantly deliver immune responses upon tumor and pathogen challenges while also being drivers of inflammation and autoimmunity in peripheral organs. Thus, this proposal has the potential to provide ground-breaking new knowledge in these areas.
Wissenschaftliches Gebiet
Schlüsselbegriffe
Programm/Programme
Thema/Themen
Finanzierungsplan
ERC-STG - Starting GrantGastgebende Einrichtung
17177 Stockholm
Schweden