Descrizione del progetto
Fare luce sulle cellule killer naturali residenti nei tessuti
Le cellule killer naturali (NK, Natural Killer) sono note per il ruolo svolto nell’immunità antitumorale, per la capacità di combattere le infezioni e per l’impatto sugli esiti delle gravidanze. Prove emergenti da modelli murini evidenziano la presenza di cellule NK residenti nei tessuti, ma i processi della loro preservazione e regolazione non sono ancora stati compresi appieno, specialmente negli esseri umani. Il progetto RESIDE, finanziato dall’UE, intende caratterizzare sottoinsiemi di cellule NK umane residenti in modo permanente nel fegato e nell’utero degli esseri umani. Gli scienziati studieranno il meccanismo alla base della residenza nei tessuti e del ricircolo di tali cellule. Utilizzando un modello di microvascolarizzazione in 3D, identificheranno inoltre i fattori che regolano l’attività delle cellule NK residenti nei tessuti.
Obiettivo
Natural killer (NK) cells contribute to tumor and antiviral immunity, regulate immune homeostasis, and impact on pregnancy success. These functions are in many instances performed in peripheral organs. Recent pioneering research in mouse models has revealed the existence and self-maintenance of tissue-resident (TR) NK cells. Although similar mechanisms are assumed to occur in humans, this has never been formally demonstrated and with our diverse infection history and active microbiota the human setting is considerably more complex. Me and my group have recently identified and characterized presumed TR NK cells in human liver and uterus. Here, we seek to challenge the prevailing concept from the mouse of how TR is formed, regulated, and maintained by taking advantage of world-unique surgical procedures for addressing lymphocyte tissue-residency, such as uterus transplantation and a novel liver surgery creating a human parabiosis situation, combined with single-cell technologies. In these settings, we will determine the detailed characteristics of subsets of NK cells permanently residing in tissues. With the different time windows as well as tissue sites presented by the clinical procedures, we will also assess kinetics of NK cell tissue repopulation. Furthermore, we aim to provide mechanistic insight into NK cell recirculation patterns by studying tissue-draining lymph fluid. Finally, we will study functional regulation of NK cell tissue-residency based on data from targeted single-cell RNAseq across multiple human tissues. The relevance of suspected and novel factors in regulating TR NK cells will be studied in a 3D microvasculature model combined with siRNA-based silencing of candidate genes. TR immune cells are poised to instantly deliver immune responses upon tumor and pathogen challenges while also being drivers of inflammation and autoimmunity in peripheral organs. Thus, this proposal has the potential to provide ground-breaking new knowledge in these areas.
Campo scientifico
Programma(i)
Argomento(i)
Meccanismo di finanziamento
ERC-STG - Starting GrantIstituzione ospitante
17177 Stockholm
Svezia