Descrizione del progetto
Modelli 3D di tessuto umano per screening medici e cosmetici
L’obiettivo del progetto PLATFORMA, finanziato dall’UE, è produrre nuove piattaforme ricche di contenuti per lo screening commerciale di cosmetici, inquinanti e nuove terapie. I partner del progetto intendono produrre due tessuti umani tridimensionali modulari, sostenuti da substrati biocompatibili ottenuti tramite stampa laser, per il monitoraggio elettrofisiologico delle condizioni della cellula. Il modulo cutaneo sensibile sarà il primo modello di tessuto cutaneo umano innervato al mondo a permettere lo studio e la sperimentazione delle risposte del sistema nervoso periferico a stimoli esterni in un contesto fisiologicamente rilevante. Il modulo muscolo motorio si presenterà come un modello tessutale neuro-muscolo-scheletrico motorio, per permettere di registrare la trasmissione neuromuscolare in maniera non invasiva e in tempo reale. Inoltre, il modulo fornirà sostegno a studi sulle atrofie muscolari e sulle malattie dei motoneuroni, grazie all’uso di cellule geneticamente modificate o derivate da pazienti e all’analisi degli effetti dei farmaci.
Obiettivo
PLATFORMA is a commercially driven collaboration to produce novel high-content phenotypic platforms for screening cosmetics, pollutants and new therapeutics. We propose to create purpose-built, 3D modular human tissues supported by laser-printed bio-compatible scaffolds from which electro-physiological status of the cells can be monitored. Each tissue module will consist of multiple different cell types. Two main modules will be developed namely a Skin-Sensory Module and a Muscle-Motor Module. Skin-Sensory Module will be a world first; a commercially available innervated human skin tissue model. It will allow studies and testing of Peripheral Nervous System (PNS) responses to external stimuli in a physiologically-relevant context. This module itself can be used to investigate activities of both dermal and nerve cells at the same time. When a stimulus is introduced on the skin layer, responses of skin cells and activities of sensory neurons (e.g. pain & itch) can be recorded simultaneously. Muscle-Motor Module is an innovative motor neuron-skeletal muscle tissue model. It allows non-invasive recordings of neuromuscular transmission in real time. It will support studies of muscular atrophies & motor neuron diseases with genetically engineered or patient derived cells and long-term monitoring and analysis of drug effects (toxicity and efficacy) with live 3D imaging and electro-physiological recordings. Partners StratiCELL and Axol Bioscience will respectively champion the exploitation of the Skin-Sensory and Muscle-Motor modules, working with early adopter customers in cosmetic and dermatologic testing drug development. The technology is derived from FET Open project MESO-BRAIN featuring 2PP fabrication of 3D scaffolds with built-in electrodes and fast maturation protocols for cultivating sensory and motor neurons from IPSc derived neural progenitors. PLATFORMA bulds upon MESO0BRAIN in the direction of market demand for physiologically-relevant test platforms.
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H2020-EIC-FETPROACT-2019
Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
B4 7ET Birmingham
Regno Unito