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Imaging the proteome at the nanoscale

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Final, optimally labeled nanobodies (opens in new window)

Final, optimally labeled nanobodies - see description of related Work Package for further details.

Final specific nanobodies (opens in new window)

Final specific nanobodies see description of related Work Package for further details

A protocol for accurate imaging (opens in new window)

A protocol for accurate imaging see description of related Work Package for further details

High-sensitivity assay (opens in new window)

Highsensitivity assay see description of related Work Package for further details

X10 protocol with optimal stability (opens in new window)

X10 protocol with optimal stability see description of related Work Package for further details

A setup capable of imaging large gels (opens in new window)

A setup capable of imaging large gels see description of related Work Package for further details

A proof-of-principle demonstration (opens in new window)

A proof-of-principle demonstration - see description of related Work Package for further details.

Semi-automated microscope (opens in new window)

Semi-automated microscope - see description of related Work Package for further details.

A microfluidic application device (opens in new window)

A microfluidic application device - see description of related Work Package for further details.

Publications

One-step nanoscale expansion microscopy reveals individual protein shapes (opens in new window)

Author(s): Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Jonas Altendorf, Daniel Mihaylov, Chi Zhang, Donatus Krah, Vanessa Imani, Russell K. W. Spencer, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Mohamad Mahdi Alawieh, Nadia Alawar, Janna Eilts, Jinyoung Kang, Alireza Soleimani, Marcus Mül
Published in: Nature Biotechnology, 2024, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-024-02431-9

Multi-foci parallelised RESOLFT nanoscopy in an extended field-of-view (opens in new window)

Author(s): Casa Moreno X, Pennacchietti F, Minet G, Damenti M, Ollech D, Barabas F, Testa I
Published in: Journal of Microscopy, 2023, ISSN 1365-2818
Publisher: John Wiley & Sons, Inc.
DOI: 10.1111/jmi.13157

Affinity microfluidics enables high-throughput protein degradation analysis in cell-freeextracts (opens in new window)

Author(s): Brio L, Wasserman D, Michaely-Barbiro E, Barazany-Gal G, Gerber D
Published in: Communications Biology, 2023, ISSN 2399-3642
Publisher: Springer Nature
DOI: 10.1038/s42003-022-04103-3

Heat denaturation enables multicolor X10-STED microscopy (opens in new window)

Author(s): Kim Ann Saal, Ali H Shaib, Nikolaos Mougios, Dagmar Crzan, Felipe Opazo, Silvio O Rizzoli
Published in: Scientific Reports, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-32524-5

An open-source microscopy framework for simultaneous control of image acquisition, reconstruction, and analysis (opens in new window)

Author(s): Casa Moreno X, Mendes Silva M, Roos J, Pennacchietti F, Norlin N, Testa I
Published in: Hardware X, 2023, ISSN 2468-0672
Publisher: Elsevier B.V.
DOI: 10.1016/j.ohx.2023.e00400

Protein nanobarcodes enable single-step multiplexed fluorescence imaging (opens in new window)

Author(s): Daniëlle de Jong-Bolm, Mohsen Sadeghi, Guobin Bao, Gabriele Klaehn, Merle Hoff, Lucas Mittelmeier, F. Buket Basmanav, Felipe Opazo, Frank Noé, Silvio O. Rizzoli
Published in: 2022
Publisher: BioRxiv
DOI: 10.1101/2022.06.03.494744

Expansion microscopy at one nanometer resolution (opens in new window)

Author(s): Ali H. Shaib, Abed Alrahman Chouaib, Vanessa Imani, Rajdeep Chowdhury, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Daniel Mihaylov, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Claudia Trenkwalder, Brit Mollenhauer, Tiago F. Outeiro, Julia Preobraschenski, Ute Becherer, Tobias Moser, Edward S. Boyden, A Radu Aricescu, Markus Sauer, Felipe Opazo, Sil
Published in: 2022
Publisher: BioRxiv
DOI: 10.1101/2022.08.03.502284

Event-triggered STED imaging (opens in new window)

Author(s): Jonatan Alvelid, Martina Damenti, Ilaria Testa
Published in: 2022
Publisher: BioRxiv
DOI: 10.1101/2021.10.26.465907

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