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Imaging the proteome at the nanoscale

Deliverables

A protocol for accurate imaging

A protocol for accurate imaging see description of related Work Package for further details

High-sensitivity assay

Highsensitivity assay see description of related Work Package for further details

X10 protocol with optimal stability

X10 protocol with optimal stability see description of related Work Package for further details

Publications

Multi-foci parallelised RESOLFT nanoscopy in an extended field-of-view

Author(s): Casa Moreno X, Pennacchietti F, Minet G, Damenti M, Ollech D, Barabas F, Testa I
Published in: Journal of Microscopy, 2023, ISSN 1365-2818
Publisher: John Wiley & Sons, Inc.
DOI: 10.1111/jmi.13157

Affinity microfluidics enables high-throughput protein degradation analysis in cell-freeextracts

Author(s): Brio L, Wasserman D, Michaely-Barbiro E, Barazany-Gal G, Gerber D
Published in: Communications Biology, 2023, ISSN 2399-3642
Publisher: Springer Nature
DOI: 10.1038/s42003-022-04103-3

Heat denaturation enables multicolor X10-STED microscopy

Author(s): Kim Ann Saal, Ali H Shaib, Nikolaos Mougios, Dagmar Crzan, Felipe Opazo, Silvio O Rizzoli
Published in: Scientific Reports, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-32524-5

An open-source microscopy framework for simultaneous control of image acquisition, reconstruction, and analysis

Author(s): Casa Moreno X, Mendes Silva M, Roos J, Pennacchietti F, Norlin N, Testa I
Published in: Hardware X, 2023, ISSN 2468-0672
Publisher: Elsevier B.V.
DOI: 10.1016/j.ohx.2023.e00400

Protein nanobarcodes enable single-step multiplexed fluorescence imaging

Author(s): Daniëlle de Jong-Bolm, Mohsen Sadeghi, Guobin Bao, Gabriele Klaehn, Merle Hoff, Lucas Mittelmeier, F. Buket Basmanav, Felipe Opazo, Frank Noé, Silvio O. Rizzoli
Published in: 2022
Publisher: BioRxiv
DOI: 10.1101/2022.06.03.494744

Expansion microscopy at one nanometer resolution

Author(s): Ali H. Shaib, Abed Alrahman Chouaib, Vanessa Imani, Rajdeep Chowdhury, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Daniel Mihaylov, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Claudia Trenkwalder, Brit Mollenhauer, Tiago F. Outeiro, Julia Preobraschenski, Ute Becherer, Tobias Moser, Edward S. Boyden, A Radu Aricescu, Markus Sauer, Felipe Opazo, Sil
Published in: 2022
Publisher: BioRxiv
DOI: 10.1101/2022.08.03.502284

Event-triggered STED imaging

Author(s): Jonatan Alvelid, Martina Damenti, Ilaria Testa
Published in: 2022
Publisher: BioRxiv
DOI: 10.1101/2021.10.26.465907

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