Imaging the proteome at the nanoscale

Informazioni relative al progetto

IMAGEOMICS

ID dell’accordo di sovvenzione: 964016

DOI

10.3030/964016

Progetto chiuso

Data della firma CE 21 Dicembre 2020

Data di avvio 1 Luglio 2021

Data di completamento 31 Dicembre 2024

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 695 981,25

Contributo UE

€ 3 695 981,25

Coordinato da

UNIVERSITAETSMEDIZIN GOETTINGEN - GEORG-AUGUST-UNIVERSITAET GOETTINGEN - STIFTUNG OEFFENTLICHEN RECHTS
Germany

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Other (2)

Final, optimally labeled nanobodies

Final, optimally labeled nanobodies - see description of related Work Package for further details.

Final specific nanobodies

Final specific nanobodies see description of related Work Package for further details

Documents, reports (3)

A protocol for accurate imaging

A protocol for accurate imaging see description of related Work Package for further details

High-sensitivity assay

Highsensitivity assay see description of related Work Package for further details

X10 protocol with optimal stability

X10 protocol with optimal stability see description of related Work Package for further details

Demonstrators, pilots, prototypes (4)

A setup capable of imaging large gels

A setup capable of imaging large gels see description of related Work Package for further details

A proof-of-principle demonstration

A proof-of-principle demonstration - see description of related Work Package for further details.

Semi-automated microscope

Semi-automated microscope - see description of related Work Package for further details.

A microfluidic application device

A microfluidic application device - see description of related Work Package for further details.

Websites, patent fillings, videos etc. (1)

Project website and logo

Establish project website and logo

Pubblicazioni

Peer reviewed articles (5)

One-step nanoscale expansion microscopy reveals individual protein shapes

Autori: Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Jonas Altendorf, Daniel Mihaylov, Chi Zhang, Donatus Krah, Vanessa Imani, Russell K. W. Spencer, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Mohamad Mahdi Alawieh, Nadia Alawar, Janna Eilts, Jinyoung Kang, Alireza Soleimani, Marcus Mül
Pubblicato in: Nature Biotechnology, 2024, ISSN 1087-0156
Editore: Nature Publishing Group
DOI: 10.1038/s41587-024-02431-9

Multi-foci parallelised RESOLFT nanoscopy in an extended field-of-view

Autori: Casa Moreno X, Pennacchietti F, Minet G, Damenti M, Ollech D, Barabas F, Testa I
Pubblicato in: Journal of Microscopy, 2023, ISSN 1365-2818
Editore: John Wiley & Sons, Inc.
DOI: 10.1111/jmi.13157

Affinity microfluidics enables high-throughput protein degradation analysis in cell-freeextracts

Autori: Brio L, Wasserman D, Michaely-Barbiro E, Barazany-Gal G, Gerber D
Pubblicato in: Communications Biology, 2023, ISSN 2399-3642
Editore: Springer Nature
DOI: 10.1038/s42003-022-04103-3

Heat denaturation enables multicolor X10-STED microscopy

Autori: Kim Ann Saal, Ali H Shaib, Nikolaos Mougios, Dagmar Crzan, Felipe Opazo, Silvio O Rizzoli
Pubblicato in: Scientific Reports, 2023, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-023-32524-5

An open-source microscopy framework for simultaneous control of image acquisition, reconstruction, and analysis

Autori: Casa Moreno X, Mendes Silva M, Roos J, Pennacchietti F, Norlin N, Testa I
Pubblicato in: Hardware X, 2023, ISSN 2468-0672
Editore: Elsevier B.V.
DOI: 10.1016/j.ohx.2023.e00400

Other (3)

Protein nanobarcodes enable single-step multiplexed fluorescence imaging

Autori: Daniëlle de Jong-Bolm, Mohsen Sadeghi, Guobin Bao, Gabriele Klaehn, Merle Hoff, Lucas Mittelmeier, F. Buket Basmanav, Felipe Opazo, Frank Noé, Silvio O. Rizzoli
Pubblicato in: 2022
Editore: BioRxiv
DOI: 10.1101/2022.06.03.494744

Expansion microscopy at one nanometer resolution

Autori: Ali H. Shaib, Abed Alrahman Chouaib, Vanessa Imani, Rajdeep Chowdhury, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Daniel Mihaylov, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Claudia Trenkwalder, Brit Mollenhauer, Tiago F. Outeiro, Julia Preobraschenski, Ute Becherer, Tobias Moser, Edward S. Boyden, A Radu Aricescu, Markus Sauer, Felipe Opazo, Sil
Pubblicato in: 2022
Editore: BioRxiv
DOI: 10.1101/2022.08.03.502284

Event-triggered STED imaging

Autori: Jonatan Alvelid, Martina Damenti, Ilaria Testa
Pubblicato in: 2022
Editore: BioRxiv
DOI: 10.1101/2021.10.26.465907