The aim of the proposal is to evaluate the effect of microbial inoculation (ectomycorrhizal fungi and Mycorrhization Help Bacteria MHB) on the root and shoot development of forest trees and European chestnut cuttings. We propose to elucidate the mechanisms involved in the improvement of rooting by microbial inoculation. The results of nursery and field experiments could be adapted and applied in practice to improve the present standard of cuttings.
We now have techniques for producing tree seedlings associated with competitive and efficient ectomycorrhizal fungi and MHB. We still have to adapt these technique to cuttings.
The groups participating in the project will work on:
1. study the physiology of IAA production by fungal and bacterial strains;
2. determine the inter and intraspectific variability of IAA synthesizing activity within fungal genera
3. isolate IAA overproducing fungal strains
4. control the rhizogenesis process and study the interactions with microorganism metabolism;
5. study the influence of microorganisms on the rooting phase and develop methods for the preinoculation of cuttings;
6. develop methods for mycorrhizal and bacterial infection of rooted cuttings in containers or in bareroot nurseries;
7. assess cuttings growth in field experiments;
8. characterise microbial strains by RFLP, PCR and isozyme analysis;
9. determine the survival of the introduced strains after field transplantation with molecular techniques of isozyme analysis;
10. study the interactions between plant and fungal genotype.
Funding SchemeCSC - Cost-sharing contracts
1017 K Copenhagen