Skip to main content

IMPROVEMENT OF THE GROWTH PERFORMANCES OF FOREST TREES (PSEUDOTSUGA MENZIESII, PICEA ABIES, PICEA SITCHENSIS, PINUS PINASTER, QUERCUS ROBUR) AND FRUIT TREES (CASTANEA SATIVA) BY MICROBIOLOGICAL INOCULATION OF CUTTINGS

Objective

The aim of the proposal is to evaluate the effect of microbial inoculation (ectomycorrhizal fungi and Mycorrhization Help Bacteria MHB) on the root and shoot development of forest trees and European chestnut cuttings. We propose to elucidate the mechanisms involved in the improvement of rooting by microbial inoculation. The results of nursery and field experiments could be adapted and applied in practice to improve the present standard of cuttings.

We now have techniques for producing tree seedlings associated with competitive and efficient ectomycorrhizal fungi and MHB. We still have to adapt these technique to cuttings.

The groups participating in the project will work on:

1. study the physiology of IAA production by fungal and bacterial strains;

2. determine the inter and intraspectific variability of IAA synthesizing activity within fungal genera

3. isolate IAA overproducing fungal strains

4. control the rhizogenesis process and study the interactions with microorganism metabolism;

5. study the influence of microorganisms on the rooting phase and develop methods for the preinoculation of cuttings;

6. develop methods for mycorrhizal and bacterial infection of rooted cuttings in containers or in bareroot nurseries;

7. assess cuttings growth in field experiments;

8. characterise microbial strains by RFLP, PCR and isozyme analysis;

9. determine the survival of the introduced strains after field transplantation with molecular techniques of isozyme analysis;

10. study the interactions between plant and fungal genotype.

Funding Scheme

CSC - Cost-sharing contracts
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

Institut National de la Recherche Agronomique (INRA)
Address
Centre De Recherche De Nancy Champenoux
54280 Seichamps
France

Participants (9)

Association Forêt-Cellulose (AFOCEL)
France
Address
164 Boulevard Haussmann
75008 Paris
Centre National du Machinisme Agricole, du Génie Rural, des Eaux et des Forêts (CEMAGREF)
France
Address
Parc De Tourvoie
92163 Antony
ESCOLA SUPERIOR AGRARIA DO INST. POLITECNICO DE BRAGANCA
Portugal
Address
Quinta De Santa Apolonia, Apt. 172
5300 Braganca
FORESTRY COMMISSION RESEARCH AGENCY
United Kingdom
Address
Alice Holt Lodge, Wrecclesham
Farnham
INST. DE RECERCA I TECNOLOGIA AGROALIMENTARIES
Spain
Address
Passeig De Gracia 44
8007 Barcelona
THE UNIVERSITY OF TUEBINGEN
Germany
Address
Auf Der Morgenstelle 1
72074 Tuebingen
UNIVERSITE CLAUDE BERNARD LYON 1
France
Address
Boulevard Du 11 Novembre 1918, 43
69622 Villeurbanne
UNIVERSITY OF COPENHAGEN
Denmark
Address
Noerregade 10
1017 K Copenhagen
Université de Paris VI (Université Pierre et Marie Curie)
France
Address
4 Place Jussieu
75252 Paris