Obiettivo This project is based on the observation that the electrophoresis step in sequencing is a major bottleneck and a considerable financial burden of genome biomedical project. It is aimed at constructing a prototype capillary array sequencer with significantly increased output and decreased operation cost, as compared with slab-gel sequencers. Capillary electrophoresis combines the following advantages as compared with slab-gels: - Capability of sustaining higher field strengths, leading to shorter separation times- Easy automation of separation matrix replacement and sample loading - Miniaturization, allowing a larger number of samples to be studied. To fully benefit from this potential, however, problems remain to be solved, because capillary electrophoresis is a much more recent technique than slab-gels. In particular, the sequencing experiments performed in capillaries up to now suffered from the short lifetime of gels cast in capillaries, which renders the operating cost prohibitive, from serious band-compression problems, and from detection methods with an insufficient sensitivity/output rate. The team will overcome these limitations, by an integrated interdisciplinary approach combining competences in biology, chemistry, chemical physics and instrumentation. The project will focus on low operation cost, high automation and robustness for routine operation, and reduce risks by exploring options on a small scale before fixing them for the full-scale prototype. It will include in particular the following innovative approaches: - - The synthesis of new acrylamido monomers, yielding sieving matrices with longer lifetime and better resistance to hydrolysis- - "Intelligent" new sieving matrices based on associating polymers, which can be switched from liquidlike behaviour (for automated replacement of used sieving medium) to gellike behaviour (for good separation properties)- - A new reporter chemistry using lanthanides, for better signal/noise ratio - - The use of pulsed-field capillary electrophoresis to increase the size range separable at high fields - - A new technology for mass production of very-low-cost capillary arrays- - An original detection scheme based on imaging techniques, well adapted to massively parallel acquisition Campo scientifico natural sciencesbiological sciencesgeneticsDNAsocial sciencessociologyindustrial relationsautomationnatural sciencesphysical sciencesmolecular and chemical physicsnatural scienceschemical scienceselectrochemistryelectrophoresisnatural sciencesbiological sciencesgeneticsgenomes Programma(i) FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998 Argomento(i) 5.1 - Gene mapping and analysis Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore Institut Curie Contributo UE Nessun dato Indirizzo 11,Rue Pierre et Marie Curie 75005 Paris Francia Mostra sulla mappa Costo totale Nessun dato Partecipanti (5) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Francia Contributo UE Nessun dato Indirizzo Rue Georges Clémenceau Université de Paris-Sud 15 91406 ORSAY Mostra sulla mappa Costo totale Nessun dato MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Germania Contributo UE Nessun dato Indirizzo Ihnestrasse 73 14195 BERLIN Mostra sulla mappa Costo totale Nessun dato ROYAL INSTITUTE OF TECHNOLOGY Svezia Contributo UE Nessun dato Indirizzo Teknikringen 30 100 44 STOCKHOLM Mostra sulla mappa Costo totale Nessun dato THE MANCHESTER METROPOLITAN UNIVERSITY Regno Unito Contributo UE Nessun dato Indirizzo Chester Street John Dalton Building M1 5GD Manchester Mostra sulla mappa Costo totale Nessun dato THE UNIVERSITY OF MILANO Italia Contributo UE Nessun dato Indirizzo LITA, Via Fratelli Cervi 93 20090 SEGRATE Mostra sulla mappa Costo totale Nessun dato