Objective
We defined and optimized a medium and a batch fermentation process at laboratory level which allows the production of a sufficient lactobacilli biomass between 5E9 till 10E9 CFU/ml of broth medium.
We demonstrated feasability of mix optimal fermentation on dextrose and lactose of both strains in a batch process, managing the inoculum rate: an equilibrated biomass was achieved with a biomass level superior to 1E9 CFU/ml for each strain.
We developed a specific microencapsulation process using a laboratory pilot spray-drying process. The bacteria, in acqueous suspension with specific low viscosity polymers and protectants, are sprayed in tiny droplets in a sparing temperature-scale air flow. The maximal biomass level achieved was 10E9 CFU/g.
The entire line (fermentation and microencapsulation-drying step) was then assembled and optimized on basis of the maximum revivifiability of the dried biomass and of stability of dried biomass during further storage.
This optimized process allowed a quite good revivifiability rate (between 40 to 70 %) and a 3-to 6-month stability of achieved biomass.
Feasability of scale up pilot production was tested on a 10 then a 20 times scale. The same results were achieved on stability and revivifiability. Nevertheless we do not succeed in collecting an important biomass in the powder (about 1E9 CFU/g). The use of multi-stage dryer will certainly permit us to optimize the final population recovery.
In addition, the scale up permits to generate productivity gain from 3 to 6 times because of drying tower height (laboratory pilote dryer course was short).
Industrial exploitation in planned in 1997 on piglets feed industry on the basis of European directives 87/153 and 94/40. Experiment on weaned piglets (1 kg/ton of 1E9 CFU/g product) has demonstrated a 9% improvement on average daily growth and 5% improvement on feed conversion (piglets from 4 to 10 weeks).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences chemical sciences polymer sciences
- social sciences economics and business economics production economics productivity
- engineering and technology industrial biotechnology bioprocessing technologies fermentation
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Programme(s)
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
22940 Plaintel
France
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