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Contenuto archiviato il 2024-06-10

Transposition mechanism and the 3'region of the i-factor, a line-like transposable element

Obiettivo



Research objectives and content
The main objective of this research project is the functional analysis of the RNase H and zinc-finger motifs located in the 3' region of I-factor-like elements. These domains, as well as the termination mode are characteristic of this subgroup of elements and are not included in the present model of transposition of LINEs. The activity of these domains has not even been tested. As a second objective, I would like to test the hypothesis that I-factor-like elements are retrotranscribed in the cytoplasm. The project will consist on the establishment of a suitable in vitro transposition system for the I-factorlike element in the laboratory of Dr. Bucheton and then, with Dr. Finnegan, the analysis of how specific mutations affect the transposition capability of the element. Training content (objective, benefit and expected impact)
This research project will be acquainted me with completely new techniques on molecular biology like cell culture, making and handling of gene constructs and perhaps protein expression and its analysis. The knowledge or this technology can be useful not only for this project but to analyse any gene. Working or two different research groups, I will benefit from the training ability of both laboratories as well as a specialised supervision on each part of the investigation.
Links with industry / industrial relevance (22)

Campo scientifico (EuroSciVoc)

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Meccanismo di finanziamento

RGI - Research grants (individual fellowships)

Coordinatore

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Contributo UE
Nessun dato
Indirizzo
Avenue de la Terrasse, BFtiment 26
91198 GIF SUR YVETTE
Francia
Costo totale
Nessun dato

Partecipanti (1)