Cel Participation of simultaneously nitrosylated and farnesylated Ras-protein in neuronal apoptosis during brain ischaemia and experimental allergic encephalomyelitis.Ras proteins are proto-oncogene products that are critical components of signalling pathways leading from cell-surface receptors to the control of cellular proliferation, differentiation or cell death. Activation of Ras is associated with ischaemia/reperfusion damage of brain or with neuroinflammatory diseases. Post-translational modification is responsible for the proper functioning of Ras. The main scientific objective of the project is the investigation of role of nitrosylation on the membrane-binding properties of Ras and downstream effectors specificity of simultaneously (farnesylated and nitrosylated) modified isoforms of Ras.The project is directed towards the following points:(a) Purification, isolation, preparation, and investigation of biological activities of double modified isoforms (simultaneously nitrosylated and farnesylated) of Ras;(b) Investigation of adhesive and phospholipid-binding properties of various modified isoforms of Ras using a highly sensitive integrated optical technique allowed detailed and sophisticated analysis of the entire binding phenomenology;(c) Investigation of the action of farnesyltransferase and nitric oxide synthase inhibitors on the induction/prevention of neuronal damage during brain ischaemia and experimental allergic encephalomyelitis in rats.The anticipated results will reveal the functionally active form of Ras which participates in different downstream processes of the cell. After successful completion of the project, it will be possible to recommend types of pharmacological drugs, which will diminish the apoptosis/necrosis of neuronal cells after ischaemia/reperfusion damage of brain or after neuroinflammation. Program(-y) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Temat(-y) 4 - Life Sciences OPEN - OPEN Call Zaproszenie do składania wniosków Data not available System finansowania Data not available Koordynator Collegium Basilea Wkład UE Brak danych Adres Hochstrasse 51 4053 Basel Szwajcaria Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (4) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Georgian Academy of Sciences Gruzja Wkład UE Brak danych Adres Gotua St 14 380060 Tbilisi Zobacz na mapie Koszt całkowity Brak danych Ministry of Health Armenia Wkład UE Brak danych Adres Orbeli St. 41 375028 Erevan Zobacz na mapie Koszt całkowity Brak danych State Medical Academy of Postgraduate Education Gruzja Wkład UE Brak danych Adres Chavchavadze Ave. 29 380079 Tbilisi Zobacz na mapie Koszt całkowity Brak danych University of P?cs Węgry Wkład UE Brak danych Adres Szigeti 12 7624 Pécs Zobacz na mapie Koszt całkowity Brak danych