Obiettivo The ATP synthase (FOF1) and the cytochrome bc1 are enzyme complexes that perform electro-mechano-chemical energy transduction. A peculiarity of both these nano-machines is intermediate mechanical reaction steps by large-scale rotation of subunits against each other.It is planned to combine(i) detection in real-time of the single enzyme rotary motion by microvideographic and spectroscopic assays;(ii) precision measurements of the intra-protein transfer of protons and electrons under single-turnover conditions and;(iii) theoretical modelling of the interplay between motion and charge transfer.On studying the ATP syntheses, the intention is to reveal how does proton transfer through the membrane portion of the ATP syntheses drive the full rotation of the central shaft of this enzyme relative to the stator subunits. It is planned to correlate the effect of several parameters (site-specific mutations in Fo and F1, specific inhibitors, pH, temperature) on the active rotation of the central shaft of FoF1, as directly visualized by single molecule monitoring techniques, with the proton displacements through FoF1 in the quasi-single-enzyme mode. On studying the bc1, the intention is to clarify the coupling between the large-scale motions at the periphery of the cytochrome-bc1 complex and the electron and proton transfer in its membrane part.Therefore it is planned to correlate the impact of various modifications of the cytochrome bc1 complex on the displacement of the peripheral FeS domain of the Rieske protein by 70° (direct visualization by new single molecule monitoring techniques would be thereby combined with indirect optical tracing via the kinetics of the cytochrome c reduction by FeS) with the proton and electron displacements inside the cytochrome-bc1 complex as monitored under the single turnover mode of the enzyme. It is planned to analyse theoretically the operation of FoF1 and bc1 by combing electrostatic calculations with the steered molecular dynamics simulations. The ultimate objective of the project is to develop mechanistic schemes of the coupling between the intramembrane charge displacements and the large-scale motions of the peripheral domains in FoF1 and in bc1. Programma(i) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Argomento(i) 4 - Life Sciences NANO - NANO : Nano-scale Materials and Structures Invito a presentare proposte Data not available Meccanismo di finanziamento Data not available Coordinatore Universitat Osnabruck Contributo UE Nessun dato Indirizzo Barbarastr. 11 49076 Osnabruck Germania Mostra sulla mappa Costo totale Nessun dato Partecipanti (3) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto M.V. Lomonosov Moscow State University Russia Contributo UE Nessun dato Indirizzo Vorobjevy Gory, Bldg. A 119899 Moscow Mostra sulla mappa Costo totale Nessun dato Russian Academy of Sciences Russia Contributo UE Nessun dato Indirizzo Leninskii Prospect 31 117071 Moscow Mostra sulla mappa Costo totale Nessun dato University of Bologna Italia Contributo UE Nessun dato Indirizzo via Irnerio 42 40126 Bologna Mostra sulla mappa Costo totale Nessun dato