Aim: to raise polyclonal antibodies in rabbits against human CFTR and CF-related (mouse) proteins for use on antibody chips, in immunocytochemistry and in Western blots.
- Rabitt poyclonal antibodies against human CFTR
- CFTR: Extracellular loops
- EnaC: Extracellular loops of a,b, and g subunits
- Rabitt polyclonal against Cytokeratins
- CK8 (Cytokeratin 8)
- CK18 (Cytokeratin 18)
- Rabitt polyclonal against human beta defensins 1,2,3,4
- Chicken polyclonal against human beta defensins 1,2,3,4
- Mouse monoclonals against huamn beta defensins 2,3,4
Mouse proteins:
- Mouse chloride channels CIC-Ka and CIC-Kb (see for selection discriminating peptides: option 3 from e-mail correspondence with Ann Andries, 18/09/03)
- Mouse barttin (=regulator of CIC-K channels, identified in mouse nasal epithelium)
- Mouse a-, b-, and g-subunits of the epithelial sodium channel ENaC
- Mouse b2-adrenergic receptor (b2-AR)
- The CFTR-regulated chloride/bicarbona te exchangers;
-- 0 mouse SLC 26 A3 (=DRA, CLD)
-- 0 mouse SLC 26 A6 (=PAT-1)
-- 0 mouse SLC 26 A9 (lung-specific)
- Mouse PDZ-adapter protein CAP-70 (binds to C-terminus of CFTR and CLD).
- Mouse CFTR chloride channel
-- 0 first extracellular loop (cf. sequence hCFTR, Chemicon Ab).
-- 0 C-terminal13 aa or cloned C-terminal fragment (80aa).
- Mouse ROMK (CFTR-regulated epithelial potassium channel in kidney and lung).
- Mouse chloride channel CIC-3.
- Mouse SGK-1 (serum-and glucocorticoid-activated protein kinase, regulates ENaC).
- Mouse chloride channel CIC-2.
- Mouse YAP-65 (=Yes-associated protein, involved in CFTR-ENaC coupling).
- Mouse D-AKAP2 (AKAP and putative CFTR regulator).