Obiettivo A network of laboratories will be formed to create a large population of gene-trap insertions in the genome of Arabidopsis thaliana to detect the spatial and temporal expression patterns of approximately 5.000 genes, and to identify and characterise the regulatory elements of these genes. Data obtained on the genes, in which transposons are inserted, will be obtained by sequencing the insertion site and this will be related to genome sequence and expression patterns in a functional genomics database. Gene expression patterns in a wide range of tissues and conditions will be defined and put in the database. The products of research will be a powerful resource to analyse the function of 20% of the genes in Arabidopsis, and these will be exploited by industrial partners.The EXOTIC project has generated 25,367 gene trap lines. This collection stands beside the CSHL collection of 18,764 gene trap and 14,204 enhancer trap lines (http://genetrap.cshl.org/servlet/CurrentStats). These collections, when available via stock centres and comprehensively described, will be a key asset for understanding gene function and gene expression in Arabidopsis. Table 2 describes the current status of gene disruption in Arabidopsis. The AtIDB database shows that 80% of the 29,000 predicted genes harbour disruptions. This represents outstanding progress towards the community goal of generating disruptions of all genes by 2005. It also shows that EU activities (GABI-KAT, Flag, Garnet and EXOTIC) have contributed nearly half of the lines available for gene disruption. It is likely that the random insertion strategies used with such success so far will not be cost effective in reaching the remaining 20% of genes, so new approaches will be needed. Here transposon insertions, which can be re-mobilised for efficient local mutagenesis, may provide users with resources to tackle genes of interest. Gene traps can also identify gene disruptions in the heterozygous state and that are expressed in the extended haploid stages, which may affect zygote viability. These factors suggest gene trap collections, such as the EXOTIC collection, will provide a key resource for tackling the remaining genes. Campo scientifico natural sciencescomputer and information sciencesdatabasesnatural sciencesbiological sciencesgeneticsgenomes Programma(i) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Argomento(i) 1.1.1.-8. - Research into genomes and diseases of genetic origin Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore AMICA SCIENCE EEIG Contributo UE Nessun dato Indirizzo Norwich Research Park John Innes Centre, Colney NR4 7UH NORWICH Regno Unito Mostra sulla mappa Costo totale Nessun dato Partecipanti (10) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Spagna Contributo UE Nessun dato Indirizzo Campus de la Universidad Autonoma de Madrid 28049 MADRID Mostra sulla mappa Costo totale Nessun dato DLF - TRIFOLIUM A/S Danimarca Contributo UE Nessun dato Indirizzo 31,Hojerupvej 31 4660 STORE HEDDINGE Mostra sulla mappa Costo totale Nessun dato FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW Belgio Contributo UE Nessun dato Indirizzo Rijvisschestraat 120 9052 ZWIJNAARDE Mostra sulla mappa Costo totale Nessun dato JOHN INNES CENTRE Regno Unito Contributo UE Nessun dato Indirizzo Norwich Research Park NR4 7UH Norwich Mostra sulla mappa Costo totale Nessun dato PLANT RESEARCH INTERNATIONAL B.V. Paesi Bassi Contributo UE Nessun dato Indirizzo Droevendaalsesteeg 1 WAGENINGEN Mostra sulla mappa Costo totale Nessun dato RISOE NATIONAL LABORATORY Danimarca Contributo UE Nessun dato Indirizzo Frederiksborgvej 399 4000 ROSKILDE Mostra sulla mappa Costo totale Nessun dato THE UNIVERSITY OF MILANO Italia Contributo UE Nessun dato Indirizzo Via Festa del Perdono 7 20122 MILANO Mostra sulla mappa Costo totale Nessun dato THE UNIVERSITY OF TUEBINGEN Germania Contributo UE Nessun dato Indirizzo Wilhelmstrasse 5 72074 TUEBINGEN Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF COPENHAGEN Danimarca Contributo UE Nessun dato Indirizzo Noerregade 10 1017 KOEBENHAVN K/COPENHAEGEN Mostra sulla mappa Costo totale Nessun dato UNIVERSITY OF ZURICH Svizzera Contributo UE Nessun dato Indirizzo 107,Zollikerstrasse 107 8008 ZURICH Mostra sulla mappa Costo totale Nessun dato