Cel A network of laboratories will be formed to create a large population of gene-trap insertions in the genome of Arabidopsis thaliana to detect the spatial and temporal expression patterns of approximately 5.000 genes, and to identify and characterise the regulatory elements of these genes. Data obtained on the genes, in which transposons are inserted, will be obtained by sequencing the insertion site and this will be related to genome sequence and expression patterns in a functional genomics database. Gene expression patterns in a wide range of tissues and conditions will be defined and put in the database. The products of research will be a powerful resource to analyse the function of 20% of the genes in Arabidopsis, and these will be exploited by industrial partners.The EXOTIC project has generated 25,367 gene trap lines. This collection stands beside the CSHL collection of 18,764 gene trap and 14,204 enhancer trap lines (http://genetrap.cshl.org/servlet/CurrentStats). These collections, when available via stock centres and comprehensively described, will be a key asset for understanding gene function and gene expression in Arabidopsis. Table 2 describes the current status of gene disruption in Arabidopsis. The AtIDB database shows that 80% of the 29,000 predicted genes harbour disruptions. This represents outstanding progress towards the community goal of generating disruptions of all genes by 2005. It also shows that EU activities (GABI-KAT, Flag, Garnet and EXOTIC) have contributed nearly half of the lines available for gene disruption. It is likely that the random insertion strategies used with such success so far will not be cost effective in reaching the remaining 20% of genes, so new approaches will be needed. Here transposon insertions, which can be re-mobilised for efficient local mutagenesis, may provide users with resources to tackle genes of interest. Gene traps can also identify gene disruptions in the heterozygous state and that are expressed in the extended haploid stages, which may affect zygote viability. These factors suggest gene trap collections, such as the EXOTIC collection, will provide a key resource for tackling the remaining genes. Dziedzina nauki natural sciencescomputer and information sciencesdatabasesnatural sciencesbiological sciencesgeneticsgenomes Program(-y) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Temat(-y) 1.1.1.-8. - Research into genomes and diseases of genetic origin Zaproszenie do składania wniosków Data not available System finansowania CSC - Cost-sharing contracts Koordynator AMICA SCIENCE EEIG Wkład UE Brak danych Adres Norwich Research Park John Innes Centre, Colney NR4 7UH NORWICH Zjednoczone Królestwo Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (10) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Hiszpania Wkład UE Brak danych Adres Campus de la Universidad Autonoma de Madrid 28049 MADRID Zobacz na mapie Koszt całkowity Brak danych DLF - TRIFOLIUM A/S Dania Wkład UE Brak danych Adres 31,Hojerupvej 31 4660 STORE HEDDINGE Zobacz na mapie Koszt całkowity Brak danych FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW Belgia Wkład UE Brak danych Adres Rijvisschestraat 120 9052 ZWIJNAARDE Zobacz na mapie Koszt całkowity Brak danych JOHN INNES CENTRE Zjednoczone Królestwo Wkład UE Brak danych Adres Norwich Research Park NR4 7UH Norwich Zobacz na mapie Koszt całkowity Brak danych PLANT RESEARCH INTERNATIONAL B.V. Niderlandy Wkład UE Brak danych Adres Droevendaalsesteeg 1 WAGENINGEN Zobacz na mapie Koszt całkowity Brak danych RISOE NATIONAL LABORATORY Dania Wkład UE Brak danych Adres Frederiksborgvej 399 4000 ROSKILDE Zobacz na mapie Koszt całkowity Brak danych THE UNIVERSITY OF MILANO Włochy Wkład UE Brak danych Adres Via Festa del Perdono 7 20122 MILANO Zobacz na mapie Koszt całkowity Brak danych THE UNIVERSITY OF TUEBINGEN Niemcy Wkład UE Brak danych Adres Wilhelmstrasse 5 72074 TUEBINGEN Zobacz na mapie Koszt całkowity Brak danych UNIVERSITY OF COPENHAGEN Dania Wkład UE Brak danych Adres Noerregade 10 1017 KOEBENHAVN K/COPENHAEGEN Zobacz na mapie Koszt całkowity Brak danych UNIVERSITY OF ZURICH Szwajcaria Wkład UE Brak danych Adres 107,Zollikerstrasse 107 8008 ZURICH Zobacz na mapie Koszt całkowity Brak danych
