Cel Influenza viruses cause a significant seasonal disease burden and continually threaten to initiate human pandemics. Antivirals are available for treatment of influenza, however drug-resistant viruses often emerge. Thus, there is urgent need to develop new antivirals with lower chances of selecting resistance. As viruses rely extensively on cellular functions, one way to minimise resistance is to target new antivirals against host factors. This concept requires a fundamental understanding of mechanisms underpinning the interplay between influenza viruses and their hosts.In this project, we will investigate the role that host SUMO pathways play during influenza virus replication. SUMO proteins are important regulators of cell signalling, and are covalently linked to other proteins in order to alter structure, localization or function. As such, SUMO conjugation regulates many diverse aspects of biology. Our own work shows that global cellular SUMOylation increases during influenza virus infection, and that virus replication is severely impaired when cells are depleted of key enzymes and components required for general SUMO conjugation. Here, we will determine what viral components trigger SUMOylation, and which specific cellular enzymes are involved. We will characterize where in the cell SUMO conjugates accumulate, and for the first time apply large-scale affinity-based quantitative proteomics to the identification of proteins that become SUMO modified during infection. A key aim will be to correlate changes to the SUMO sub-proteome with the function of specific host SUMO-modifying enzymes, thereby establishing the mechanistic role of these modifications during virus replication.Understanding basic mechanisms underlying SUMOylation during influenza virus infection will provide new insights into the fundamental biology of these important pathogens. The work could also lead to identification of key cellular pathways that can be exploited as novel therapeutic targets Dziedzina nauki natural sciencesbiological sciencesmicrobiologyvirologymedical and health scienceshealth sciencespublic healthepidemiologypandemicsmedical and health scienceshealth sciencesinfectious diseasesRNA virusesinfluenzanatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantivirals Program(-y) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) ERC-SG-LS6 - ERC Starting Grant - Immunity and infection Zaproszenie do składania wniosków ERC-2013-StG Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-SG - ERC Starting Grant Instytucja przyjmująca University of Zurich Wkład UE € 1 482 452,00 Adres RAMISTRASSE 71 8006 ZURICH Szwajcaria Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Michael Biller (Mr.) Kierownik naukowy Benjamin Hale (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych Beneficjenci (2) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko University of Zurich Szwajcaria Wkład UE € 1 482 452,00 Adres RAMISTRASSE 71 8006 ZURICH Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Michael Biller (Mr.) Kierownik naukowy Benjamin Hale (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych UNIVERSITY OF GLASGOW Zakończenie uczestnictwa Zjednoczone Królestwo Wkład UE Brak danych Adres UNIVERSITY AVENUE G12 8QQ Glasgow Zobacz na mapie Region Scotland West Central Scotland Glasgow City Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Joseph Galloway (Mr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych