Cel Abiotic environmental stresses such as extreme temperatures represent serious threats to plants and are a main reason for significant agricultural crop losses every year. Hence, an understanding of the molecular consequences of stress exposure and the underlying principles of plant stress responses are fundamental questions in biology and provide the basis for the genetic engineering of stress resistant crop plants. In particular, high temperature stress leads to an increase in protein unfolding, misfolding, and aggregation and thus challenges proteome homeostasis (proteostasis). As a response, cells increase the expression of molecular chaperones to maintain proteostasis by counteracting protein misfolding and aggregation.The overall goal of this study is to globally identify properties of fragile proteins in plant proteomes challenged by heat-shock exposure. We will use the unicellular green algae Chlamydomonas reinhardtii and Nannochloropsis sp. as model organisms to monitor protein misfolding and aggregation under physiological conditions, mild stress and severe temperature stress. We will apply quantitative proteomics and bioinformatics to reveal physicochemical properties characteristic for proteins prone to misfolding and aggregation. By this, we will further identify proteins that have a high dependence on protection by molecular chaperones. Based on this system-wide analysis individual proteins will be engineered with the goal to optimize their conformational stability for improved stress tolerance. Dziedzina nauki natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesmicrobiologyphycologymedical and health sciencesmedical biotechnologygenetic engineeringnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsprotein foldingmedical and health sciencesbasic medicinephysiologyhomeostasis Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) FP7-PEOPLE-2013-CIG - Marie-Curie Action: "Career Integration Grants" Zaproszenie do składania wniosków FP7-PEOPLE-2013-CIG Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-CIG - Support for training and career development of researcher (CIG) Koordynator RHEINLAND-PFALZISCHE TECHNISCHE UNIVERSITAT Wkład UE € 100 000,00 Adres GOTTLIEB DAIMLER STRASSE 67663 Kaiserslautern Niemcy Zobacz na mapie Region Rheinland-Pfalz Rheinhessen-Pfalz Kaiserslautern, Kreisfreie Stadt Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Jörg Hansen (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
