Obiettivo An impressive variety of motile and morphogenetic processes are driven by site-directed polarized asssembly of actin filaments. In the past ten years, breathtaking advances coming from cell biology, cell biophysics, and biochemistry have brought insight into the molecular bases for production of force and movement by site-directed actin polymerization. Yet, we do not know, with the detail sufficient to understand how force is produced, by which molecular mechanisms the filaments are nucleated or created by branching. We do not know by which elementary steps insertional polymerization of barbed ends of filaments against the membrane is performed by different protein machineries, nor how these machineries work in a coordinated fashion. Here we propose a multiscale and interdisciplinary approach of the mechanisms used by the major actin nucleators to organize the motile response of actin. The elementary reactions involved in the processive walk of formin at the growing barbed ends of filaments and the role of ATP hydrolysis in force production will be analyzed by a combination of biochemical solution studies and physical methods using functionalized GUVs and optical tweezers. The multifunctionality of WH2 domains involved in actin sequestration, filament nucleation severing and processive elongation will be similarly examined in an interdisciplinary perspective from structural biology at atomic resolution to physics at the mesoscopic scale. Biochemical and structural methods and single molecule measurements (TIRFM) will shed light into the elementary steps and structural mechanism of filament branching. Biomimetic assays with functionalized GUVs associated with biophysical methods like FRAP or fluorescence correlation spectroscopy will elucidate how different filament initiating machineries segregate in the membrane as a consequence of their interactions with growing filaments and function in a coordinated fashion during actin-based motility. Campo scientifico natural sciencesphysical sciencescondensed matter physicssoft matter physicsnatural sciencesbiological sciencescell biologynatural sciencesbiological sciencesbiophysicsnatural sciencesbiological sciencesmolecular biologystructural biologynatural sciencesphysical sciencesopticsspectroscopy Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-AG-ID1 - ERC Advanced Grant Interdisciplinary Panel Invito a presentare proposte ERC-2009-AdG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-AG - ERC Advanced Grant Istituzione ospitante CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Contributo UE € 2 194 195,00 Indirizzo RUE MICHEL ANGE 3 75794 Paris Francia Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Ricercatore principale Marie-France Carlier épouse Pantaloni (Dr.) Contatto amministrativo Véronique Debisschop (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (3) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Francia Contributo UE € 2 194 195,00 Indirizzo RUE MICHEL ANGE 3 75794 Paris Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Ricercatore principale Marie-France Carlier épouse Pantaloni (Dr.) Contatto amministrativo Véronique Debisschop (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITAT BONN Partecipazione conclusa Germania Contributo UE € 112 113,14 Indirizzo REGINA PACIS WEG 3 53113 Bonn Mostra sulla mappa Regione Nordrhein-Westfalen Köln Bonn, Kreisfreie Stadt Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Daniela Hasenpusch Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato TECHNISCHE UNIVERSITAET BRAUNSCHWEIG Germania Contributo UE € 127 886,86 Indirizzo UNIVERSITAETSPLATZ 2 38106 Braunschweig Mostra sulla mappa Regione Niedersachsen Braunschweig Braunschweig, Kreisfreie Stadt Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Klemens Rottner (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato