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Human mini hearts: looking for culprits and victims in cardiac disease

Descrizione del progetto

Modelli di patologie cardiache

Le cardiopatie, inclusa l’aritmia, sono spesso silenziose fino a quando un paziente non sperimenta determinati sintomi, come il dolore e il malessere al petto. Essere in grado di prevedere le patologie cardiache e prevenire l’insufficienza cardiaca è fondamentale, ma al momento non esistono i modelli necessari per studiare i meccanismi fisiopatologici. Il progetto Mini-HEART, finanziato dall’UE, propone di generare microtessuti cardiaci utilizzando cellule staminali pluripotenti indotte (hiPSC) umane, differenziate in cellule cardiache in vitro. I ricercatori ricapitoleranno la malattia cardiaca in questi modelli hiPSC e decifreranno il ruolo dei diversi tipi di cellule nel fenotipo cardiaco. Inoltre, testeranno nuovi approcci terapeutici per le patologie cardiache.

Obiettivo

Cardiac disease causes morbidity and mortality as frequently as cancer. Predicting cardiac arrhythmia and cardiac failure, understanding multicellular (patho)physiological mechanisms, and devising new treatments represent unmet needs in the field. Human induced pluripotent stem cells (hiPSC) could revolutionise the way we study human disease but unfortunately, they still fall short in recapitulating variable phenotypes and complex cardiovascular diseases. This is partly due to functional immaturity of hiPSC-derived cardiac tissues, shortage of methods for accurate functional analysis and inability to identify cell-type specific contributions to disease pathology. I will address these challenges in Mini-HEART. We recently assembled novel three-dimensional multi-cellular cardiac microtissues as an important step towards full maturation and used these to demonstrate that cardiac disease mutations might directly affect non-myocyte cardiac cells. However, application of hiPSC microtissues to precision medicine remains to emerge. Using a multidisciplinary approach, I will combine isogenic hiPSC, their differentiation into distinct cell types of the heart, multifaceted biophysical assays, and tissue engineering to prove cell-type causality of disease and identify new molecules targeting the culprit cells to rescue the disease phenotype. I plan to use our unique complex hiPSC-cardiac microtissue to 1) identify and synthetically enhance maturation mechanisms; 2) reveal late arrhythmic and fibrotic phenotypes; 3) dissect cell-type specific contributions to complex cardiac diseases, including the role of macrophages and sympathetic nerves; 4) test two therapeutic strategies based on drug-tailoring and gene editing, to modulate the disease phenotype. Together, Mini-HEART will create new opportunities for designing novel biomedical tools to i) capture phenotypic changes, ii) reveal (patho)physiological mechanisms and ii) develop new therapeutic approaches for heart disease.

Campo scientifico (EuroSciVoc)

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.

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Meccanismo di finanziamento

ERC-COG - Consolidator Grant

Istituzione ospitante

UNIVERSITA DEGLI STUDI DI PADOVA
Contribution nette de l'UE
€ 1 500 000,00
Indirizzo
VIA 8 FEBBRAIO 2
35122 Padova
Italia

Mostra sulla mappa

Regione
Nord-Est Veneto Padova
Tipo di attività
Higher or Secondary Education Establishments
Collegamenti
Costo totale
€ 1 500 000,00

Beneficiari (2)