Projektbeschreibung
Analyse einzelner Bakterienzellen für gesteigerte Methanproduktion
Bei der anaeroben Vergärung zersetzen Mikroorganismen in Abwesenheit von Sauerstoff organisches Material und produzieren dabei Biogas, vor allem Methan. Die mikrobielle Erzeugung von Treibstoff aus organischen Reststoffen wird den Übergang von fossilen Brennstoffen hin zu saubereren Energien fördern und Emissionen senken. Für eine kostengünstige großindustrielle Treibstoffproduktion und einen entsprechenden Investitionsanreiz müssen diese bakteriellen Prozesse aber noch verstärkt werden. Mit hochtechnologischen Verfahren der Einzelzellgenetik analysiert das EU-finanzierte Projekt UnrAD im Detail die Zusammensetzung bakterieller Gemeinschaften. Damit sollen Spezialisierungen und Wechselwirkungen zwischen methanerzeugenden Bakterien aufgezeigt und so letztlich die Ausbeute gesteigert werden.
Ziel
Anaerobic digestion, a technology able to use waste residuals to produce clean renewable energy in form of methane, is a powerful tool to revert the unequivocal environmental deterioration and represent one of the clearest examples of how research and innovation can turn undesirable residual like waste into an ecological and economical asset. Recent advances made feasible the process at ambient temperature, feasibility that might increase the net production since does not need heat supplementation. Among the different trace metals involved in the anaerobic degradation processes, cobalt has been shown one of the most important since it acts as a cofactor for enzymes involved in the metabolic pathways of the methanogenesis from acetate. Therefore an adequate balance of cobalt could improve the anaerobic biological response, enhancing the rate and yield of methane. High throughput single-cell transcriptomic and third generation sequencing provide an unprecedented opportunity to understand the mechanisms inducing this enhanced yield. The first allows trancriptome analysis at the level of individual cells giving the possibility to understand the interplay of transcripts within single organisms, the specialization of different members from the community or the heterogeneity in term of transcriptomic status of every species while the second enable the possibility recover full length transcripts increasing resolution. The overall objective of UnrAD is to employ these two cutting-edge technologies combined to study the role of temperature and cobalt supplementation in anaerobic digestion and identify specializations and interactions among the different bacterial community members. The findings from this project would lead to a more efficient anaerobic digestion and will be beneficial for the to the industries in the field and the whole society.
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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordinator
28006 Madrid
Spanien