Descrizione del progetto
Comprendere il ruolo dei composti neuroattivi della dieta nello sviluppo delle malattie neurodegenerative
I legami fra dieta, intestino e malattie neurodegenerative sono sempre più evidenti. Il progetto NeuroTOm, finanziato dal programma di azioni Marie Skłodowska-Curie, si propone di comprendere il significato dell’esposizione ai composti neuroattivi della dieta. Utilizzando il pomodoro come fonte alimentare, lo studio chiarirà il ruolo di queste sostanze chimiche nell’asse intestino-cervello e nello sviluppo di malattie neurodegenerative dopo la digestione. I composti neuroprotettivi e neurodistruttivi dei pomodori biologici, convenzionali e trasformati saranno caratterizzati mediante spettrometria di massa. Verranno inoltre utilizzati modelli di colon in vitro per studiare il destino di questi composti nell’intestino e individuare i metaboliti intestinal-microbici e il loro effetto sulle cellule dell’epitelio intestinale.
Obiettivo
Neurodegenerative (ND) diseases are debilitating and largely untreatable conditions that affect millions worldwide, including 7+ million Europeans. Although there remains no cure, there is growing evidence of a correlation between diet, gut, and ND diseases but a deeper understanding of what happens to neuroactive compounds once digested and their role in the communication between gut and brain is still missing. In response, NeuroTOm will answer complex questions about human exposure to neuroactive compounds (using tomato as a model food), such as what happens to these chemicals during digestion and their role in the gut-brain axis and ND disease development. It will use advanced mass spectrometry-based techniques to characterise neuroprotective and neuro-disrupting compounds in tomatoes produced organically, conventionally, and processed. An in vitro batch colon model will then be applied to investigate the fate of these compounds in the gut microbiome and identify gut-microbial metabolites. A step towards more realistic conditions will be made by investigating how the presence of neuro-disrupting compounds influences the bioavailability and beneficial effects of neuroprotective compounds in the gut. Selected compounds will also be tested using an innovative in vitro 3D colon (organoid) model to determine the role of neuroprotective/disruptive compounds and their gut microbial metabolites on intestinal epithelium cells. The proposed research is highly interdisciplinary and encompasses analytical and food chemistry, holistic and multi-omics approaches, nutritional biochemistry, biotechnology, and aspects of neuroscience. The goal of NeuroTOm is to extend the knowledge of diet–gut microbiome–host (brain) interactions and will contribute towards preventing or alleviating the burden of ND disease.
Campo scientifico
Programma(i)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Meccanismo di finanziamento
HORIZON-AG-UN - HORIZON Unit GrantCoordinatore
38098 San Michele All'Adige
Italia