ERA Chair Holder to establish the electron cryo-microscopy analysis of cellular and artificial nanomachines

Project Information

hot4cryo

Grant agreement ID: 101086665

DOI

10.3030/101086665

EC signature date 14 October 2022

Start date 1 March 2023

End date 29 February 2028

Funded under

• Widening participation and spreading excellence

Total cost

€ 2 499 921,25

EU contribution

€ 2 499 921,00

Coordinated by

ETHNIKO IDRYMA EREVNON
Greece

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Websites, patent fillings, videos etc. (1)

DEC Strategy and Plan

DEC Strategy and Plan: This deliverable will also be communicated at month 42.

Documents, reports (2)

Computational protocols for cryo-EM image analysis of ERA Chair specimen

Computational protocols for cryo-EM image analysis of ERA Chair specimen.

Data Management Plan (DMP)

Data Management Plan (DMP).

Publications

Peer reviewed articles (19)

Enabling cryo-EM density interpretation from yeast native cell extracts by proteomics data and AlphaFold structures

Author(s): Tüting C, Schmidt L, Skalidis I, Sinz A, Kastritis PL.
Published in: Proteomics, Issue Apr 4:e2200096, 2023, ISSN 1615-9861
Publisher: Wiley
DOI: 10.1002/pmic.202200096

Self-supervised learning for generalizable particle picking in cryo-EM micrographs

Author(s): Andreas Zamanos, Panagiotis Koromilas, Giorgos Bouritsas, Panagiotis L. Kastritis, Yannis Panagakis
Published in: Cell Reports Methods, Issue 5, 2025, ISSN 2667-2375
Publisher: Elsevier BV
DOI: 10.1016/J.CRMETH.2025.101089

The human touch: Utilizing AlphaFold 3 to analyze structures of endogenous metabolons

Author(s): Toni K. Träger, Christian Tüting, Panagiotis L. Kastritis
Published in: Structure, Issue 32, 2024, ISSN 0969-2126
Publisher: Elsevier BV
DOI: 10.1016/J.STR.2024.08.018

Protein-Rich Rafts in Hybrid Polymer/Lipid Giant Unilamellar Vesicles

Author(s): Nika Otrin*, Lado Otrin, Claudia Bednarz, Toni K. Träger, Farzad Hamdi, Panagiotis L. Kastritis, Ivan Ivanov, and Kai Sundmacher
Published in: Biomacromolecules, Issue Feb 12;25(2):778-791, 2024, ISSN 1525-7797
Publisher: ACS Publications
DOI: 10.1021/acs.biomac.3c00972

Solid-state NMR reveals mixed side-chain organization across pores in amphiphilic covalent organic frameworks

Author(s): Simona Bassoli, Alexey Krushelnitsky, Farzad Hamdi, Jonas Lührs, Panagiotis L. Kastritis, Daniel Sebastiani, Kay Saalwächter, Frederik Haase
Published in: Journal of Materials Chemistry A, Issue 14, 2025, ISSN 2050-7488
Publisher: Royal Society of Chemistry (RSC)
DOI: 10.1039/D5TA06558E

Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals

Author(s): Alaa Shaikhqasem, Farzad Hamdi, Lisa Machner, Christoph Parthier, Constanze Breithaupt, Fotis L. Kyrilis, Stephan M. Feller, Panagiotis L. Kastritis, Milton T. Stubbs
Published in: Acta Crystallographica Section D Structural Biology, Issue 82, 2026, ISSN 2059-7983
Publisher: International Union of Crystallography (IUCr)
DOI: 10.1107/S2059798325011258

Cryo-EM structure of a plant photosystem II supercomplex with light-harvesting protein Lhcb8 and α-tocopherol

Author(s): Opatíková M, Semchonok DA, Kopečný D, Ilík P, Pospíšil P, Ilíková I, Roudnický P, Zeljković SĆ, Tarkowski P, Kyrilis FL, Hamdi F, Kastritis PL, Kouřil R.
Published in: Nature Plants, Issue Aug;9(8):1359-1369, 2023, ISSN 2055-0278
Publisher: Nature Press
DOI: 10.1038/s41477-023-01483-0

Cryo-EM of a heterogeneous biochemical fraction elucidates multiple protein complexes from a multicellular thermophilic eukaryote

Author(s): Semchonok DA, Kyrilis FL, Hamdi F, Kastritis PL.
Published in: J Struct Biol: X, Issue Aug 9;8:100094, 2023, ISSN 2590-1524
Publisher: Elsevier
DOI: 10.1016/j.yjsbx.2023.100094

Disorder-to-order active site capping regulates the rate-limiting step of the inositol pathway

Author(s): Toni K. Träger, Fotis L. Kyrilis, Farzad Hamdi, Christian Tüting, Marie Alfes, Tommy Hofmann, Carla Schmidt, Panagiotis L. Kastritis
Published in: Proceedings of the National Academy of Sciences, Issue 121, 2024, ISSN 0027-8424
Publisher: Proceedings of the National Academy of Sciences
DOI: 10.1073/PNAS.2400912121

Form follows function – structural interplay between DCL1 and pri-miRNAs

Author(s): Cecile R. Scholl, Lars Grosch, Jana Baradei, Panagiotis L. Kastritis, Clara T. Schoeder, Sascha Laubinger
Published in: Trends in Plant Science, Issue 30, 2025, ISSN 1360-1385
Publisher: Elsevier BV
DOI: 10.1016/J.TPLANTS.2025.07.016

Cryo-EM structure of photosystem II supercomplex from a green microalga with extreme phototolerance

Author(s): Rameez Arshad, Ioannis Skalidis, David Kopečný, Sylva Brabencová, Monika Opatíková, Petr Ilík, Pavel Pospíšil, Farzad Hamdi, Sanja Ćavar Zeljković, Martina Kopečná, Pavel Roudnický, Dušan Lazár, Eduard Elias, Roberta Croce, Panagiotis L. Kastritis, Roman Kouřil
Published in: Nature Communications, Issue 17, 2026, ISSN 2041-1723
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-65861-2

Conserved hydrophilic checkpoints tune FocA-mediated formate:H+ symport

Author(s): Christian Tüting, Kevin Janson, Michelle Kammel, Christian Ihling, Jana Lorenz, Fotis L. Kyrilis, Farzad Hamdi, Christopher Erdmann, Andrea Sinz, R. Gary Sawers, Panagiotis L. Kastritis
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-65159-3

Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts

Author(s): Lisa Schmidt, Christian Tüting, Fotis L. Kyrilis, Farzad Hamdi, Dmitry A. Semchonok, Gerd Hause, Annette Meister, Christian Ihling, Milton T. Stubbs, Andrea Sinz & Panagiotis L. Kastritis
Published in: Communications Biology, Issue May 10;7(1):557, 2024, ISSN 2399-3642
Publisher: Springer Nature
DOI: 10.1038/s42003-024-06204-7

The Fluorescence Yield of Red pH Sensitive Proteins is Mainly Determined by Stability and Internal Water Contact of the Chromophore

Author(s): Franz-Josef Schmitt, Amna Shah Mehmood, Hoang Trong Phan, Christian Tüting, Fabian Rieder, Alina-Sophie Henneberger, Farzin Ghane Golmohamadi, Panagiotis L. Kastritis, Jan Laufer
Published in: European Conferences on Biomedical Optics 2025, 2025, ISSN 2059-7983
Publisher: Optica Publishing Group
DOI: 10.1107/S2059798325011258

Structural biology in cellulo: Minding the gap between conceptualization and realization

Author(s): Fotis L Kyrilis, Jason K K Low, Joel P Mackay, Panagiotis L Kastritis
Published in: Current Opinion Structural Biology, Issue May 23:87:102843, 2024, ISSN 1879-033X
Publisher: Elsevier
DOI: 10.1016/j.sbi.2024.102843

Rational Design of New Biocompatible Near-Infrared Conjugated Polymer Nanoparticles for Biomedical Applications

Author(s): Aristea Pavlou, Athanasios Katsouras, Maria Markou, Alkmini Nega, Sofia Bellou, Panagiota Koralli, Andriana Schiza, Nikolaos Chalmpes, Vasilis G. Gregoriou, Antonia Dimitrakopoulou-Strauss, Theodore Fotsis, Apostolos Avgeropoulos, Michael G. Siskos, Carol Murphy, Christos L. Chochos
Published in: Macromolecules, Issue 59, 2026, ISSN 0024-9297
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACS.MACROMOL.5C01788

Structural analysis of an endogenous 4-megadalton succinyl-CoA-generating metabolon

Author(s): Skalidis I, Kyrilis FL, Tüting C, Hamdi F, Träger TK, Belapure J, Hause G, Fratini M, O'Reilly FJ, Heilmann I, Rappsilber J, Kastritis PL.
Published in: Commun Biol, Issue May 22;6(1):552., 2023, ISSN 2399-3642
Publisher: Nature Press
DOI: 10.1038/s42003-023-04885-0

Cracking the code of cellular protein-protein interactions: Alphafold and whole-cell crosslinking to the rescue

Author(s): Träger T, Kastritis PL
Published in: Mol Syst Biol, Issue Apr 12;19(4):e11587, 2023, ISSN 1744-4292
Publisher: Nature Publishing Group
DOI: 10.15252/msb.202311587

Evidence of Heteroepitaxy and Solid Solutions in Lattice Matched Ternary Covalent Organic Framework Systems

Author(s): Alena Winter, Juliane Lange, Farzad Hamdi, Panagiotis L. Kastritis, Frederik Haase
Published in: Journal of the American Chemical Society, Issue 147, 2025, ISSN 0002-7863
Publisher: American Chemical Society (ACS)
DOI: 10.1021/JACS.5C02502