ERA Chair Holder to establish the electron cryo-microscopy analysis of cellular and artificial nanomachines

Informazioni relative al progetto

hot4cryo

ID dell’accordo di sovvenzione: 101086665

DOI

10.3030/101086665

Data della firma CE 14 Ottobre 2022

Data di avvio 1 Marzo 2023

Data di completamento 29 Febbraio 2028

Finanziato da

• Widening participation and spreading excellence

Costo totale

€ 2 499 921,25

Contributo UE

€ 2 499 921,00

Coordinato da

ETHNIKO IDRYMA EREVNON
Greece

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Websites, patent fillings, videos etc. (1)

DEC Strategy and Plan

DEC Strategy and Plan: This deliverable will also be communicated at month 42.

Documents, reports (2)

Computational protocols for cryo-EM image analysis of ERA Chair specimen

Computational protocols for cryo-EM image analysis of ERA Chair specimen.

Data Management Plan (DMP)

Data Management Plan (DMP).

Pubblicazioni

Peer reviewed articles (19)

Enabling cryo-EM density interpretation from yeast native cell extracts by proteomics data and AlphaFold structures

Autori: Tüting C, Schmidt L, Skalidis I, Sinz A, Kastritis PL.
Pubblicato in: Proteomics, Numero Apr 4:e2200096, 2023, ISSN 1615-9861
Editore: Wiley
DOI: 10.1002/pmic.202200096

Self-supervised learning for generalizable particle picking in cryo-EM micrographs

Autori: Andreas Zamanos, Panagiotis Koromilas, Giorgos Bouritsas, Panagiotis L. Kastritis, Yannis Panagakis
Pubblicato in: Cell Reports Methods, Numero 5, 2025, ISSN 2667-2375
Editore: Elsevier BV
DOI: 10.1016/J.CRMETH.2025.101089

The human touch: Utilizing AlphaFold 3 to analyze structures of endogenous metabolons

Autori: Toni K. Träger, Christian Tüting, Panagiotis L. Kastritis
Pubblicato in: Structure, Numero 32, 2024, ISSN 0969-2126
Editore: Elsevier BV
DOI: 10.1016/J.STR.2024.08.018

Protein-Rich Rafts in Hybrid Polymer/Lipid Giant Unilamellar Vesicles

Autori: Nika Otrin*, Lado Otrin, Claudia Bednarz, Toni K. Träger, Farzad Hamdi, Panagiotis L. Kastritis, Ivan Ivanov, and Kai Sundmacher
Pubblicato in: Biomacromolecules, Numero Feb 12;25(2):778-791, 2024, ISSN 1525-7797
Editore: ACS Publications
DOI: 10.1021/acs.biomac.3c00972

Solid-state NMR reveals mixed side-chain organization across pores in amphiphilic covalent organic frameworks

Autori: Simona Bassoli, Alexey Krushelnitsky, Farzad Hamdi, Jonas Lührs, Panagiotis L. Kastritis, Daniel Sebastiani, Kay Saalwächter, Frederik Haase
Pubblicato in: Journal of Materials Chemistry A, Numero 14, 2025, ISSN 2050-7488
Editore: Royal Society of Chemistry (RSC)
DOI: 10.1039/D5TA06558E

Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals

Autori: Alaa Shaikhqasem, Farzad Hamdi, Lisa Machner, Christoph Parthier, Constanze Breithaupt, Fotis L. Kyrilis, Stephan M. Feller, Panagiotis L. Kastritis, Milton T. Stubbs
Pubblicato in: Acta Crystallographica Section D Structural Biology, Numero 82, 2026, ISSN 2059-7983
Editore: International Union of Crystallography (IUCr)
DOI: 10.1107/S2059798325011258

Cryo-EM structure of a plant photosystem II supercomplex with light-harvesting protein Lhcb8 and α-tocopherol

Autori: Opatíková M, Semchonok DA, Kopečný D, Ilík P, Pospíšil P, Ilíková I, Roudnický P, Zeljković SĆ, Tarkowski P, Kyrilis FL, Hamdi F, Kastritis PL, Kouřil R.
Pubblicato in: Nature Plants, Numero Aug;9(8):1359-1369, 2023, ISSN 2055-0278
Editore: Nature Press
DOI: 10.1038/s41477-023-01483-0

Cryo-EM of a heterogeneous biochemical fraction elucidates multiple protein complexes from a multicellular thermophilic eukaryote

Autori: Semchonok DA, Kyrilis FL, Hamdi F, Kastritis PL.
Pubblicato in: J Struct Biol: X, Numero Aug 9;8:100094, 2023, ISSN 2590-1524
Editore: Elsevier
DOI: 10.1016/j.yjsbx.2023.100094

Disorder-to-order active site capping regulates the rate-limiting step of the inositol pathway

Autori: Toni K. Träger, Fotis L. Kyrilis, Farzad Hamdi, Christian Tüting, Marie Alfes, Tommy Hofmann, Carla Schmidt, Panagiotis L. Kastritis
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 121, 2024, ISSN 0027-8424
Editore: Proceedings of the National Academy of Sciences
DOI: 10.1073/PNAS.2400912121

Form follows function – structural interplay between DCL1 and pri-miRNAs

Autori: Cecile R. Scholl, Lars Grosch, Jana Baradei, Panagiotis L. Kastritis, Clara T. Schoeder, Sascha Laubinger
Pubblicato in: Trends in Plant Science, Numero 30, 2025, ISSN 1360-1385
Editore: Elsevier BV
DOI: 10.1016/J.TPLANTS.2025.07.016

Cryo-EM structure of photosystem II supercomplex from a green microalga with extreme phototolerance

Autori: Rameez Arshad, Ioannis Skalidis, David Kopečný, Sylva Brabencová, Monika Opatíková, Petr Ilík, Pavel Pospíšil, Farzad Hamdi, Sanja Ćavar Zeljković, Martina Kopečná, Pavel Roudnický, Dušan Lazár, Eduard Elias, Roberta Croce, Panagiotis L. Kastritis, Roman Kouřil
Pubblicato in: Nature Communications, Numero 17, 2026, ISSN 2041-1723
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-65861-2

Conserved hydrophilic checkpoints tune FocA-mediated formate:H+ symport

Autori: Christian Tüting, Kevin Janson, Michelle Kammel, Christian Ihling, Jana Lorenz, Fotis L. Kyrilis, Farzad Hamdi, Christopher Erdmann, Andrea Sinz, R. Gary Sawers, Panagiotis L. Kastritis
Pubblicato in: Nature Communications, Numero 16, 2025, ISSN 2041-1723
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-65159-3

Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts

Autori: Lisa Schmidt, Christian Tüting, Fotis L. Kyrilis, Farzad Hamdi, Dmitry A. Semchonok, Gerd Hause, Annette Meister, Christian Ihling, Milton T. Stubbs, Andrea Sinz & Panagiotis L. Kastritis
Pubblicato in: Communications Biology, Numero May 10;7(1):557, 2024, ISSN 2399-3642
Editore: Springer Nature
DOI: 10.1038/s42003-024-06204-7

The Fluorescence Yield of Red pH Sensitive Proteins is Mainly Determined by Stability and Internal Water Contact of the Chromophore

Autori: Franz-Josef Schmitt, Amna Shah Mehmood, Hoang Trong Phan, Christian Tüting, Fabian Rieder, Alina-Sophie Henneberger, Farzin Ghane Golmohamadi, Panagiotis L. Kastritis, Jan Laufer
Pubblicato in: European Conferences on Biomedical Optics 2025, 2025, ISSN 2059-7983
Editore: Optica Publishing Group
DOI: 10.1107/S2059798325011258

Structural biology in cellulo: Minding the gap between conceptualization and realization

Autori: Fotis L Kyrilis, Jason K K Low, Joel P Mackay, Panagiotis L Kastritis
Pubblicato in: Current Opinion Structural Biology, Numero May 23:87:102843, 2024, ISSN 1879-033X
Editore: Elsevier
DOI: 10.1016/j.sbi.2024.102843

Rational Design of New Biocompatible Near-Infrared Conjugated Polymer Nanoparticles for Biomedical Applications

Autori: Aristea Pavlou, Athanasios Katsouras, Maria Markou, Alkmini Nega, Sofia Bellou, Panagiota Koralli, Andriana Schiza, Nikolaos Chalmpes, Vasilis G. Gregoriou, Antonia Dimitrakopoulou-Strauss, Theodore Fotsis, Apostolos Avgeropoulos, Michael G. Siskos, Carol Murphy, Christos L. Chochos
Pubblicato in: Macromolecules, Numero 59, 2026, ISSN 0024-9297
Editore: American Chemical Society (ACS)
DOI: 10.1021/ACS.MACROMOL.5C01788

Structural analysis of an endogenous 4-megadalton succinyl-CoA-generating metabolon

Autori: Skalidis I, Kyrilis FL, Tüting C, Hamdi F, Träger TK, Belapure J, Hause G, Fratini M, O'Reilly FJ, Heilmann I, Rappsilber J, Kastritis PL.
Pubblicato in: Commun Biol, Numero May 22;6(1):552., 2023, ISSN 2399-3642
Editore: Nature Press
DOI: 10.1038/s42003-023-04885-0

Cracking the code of cellular protein-protein interactions: Alphafold and whole-cell crosslinking to the rescue

Autori: Träger T, Kastritis PL
Pubblicato in: Mol Syst Biol, Numero Apr 12;19(4):e11587, 2023, ISSN 1744-4292
Editore: Nature Publishing Group
DOI: 10.15252/msb.202311587

Evidence of Heteroepitaxy and Solid Solutions in Lattice Matched Ternary Covalent Organic Framework Systems

Autori: Alena Winter, Juliane Lange, Farzad Hamdi, Panagiotis L. Kastritis, Frederik Haase
Pubblicato in: Journal of the American Chemical Society, Numero 147, 2025, ISSN 0002-7863
Editore: American Chemical Society (ACS)
DOI: 10.1021/JACS.5C02502