FUnctional Nucleic Acids as Versatile SMart BUilding BLocks in Non-ConventIonal SolvenTs

Informazioni relative al progetto

FUNAMBULIST

ID dell’accordo di sovvenzione: 101099652

DOI

10.3030/101099652

Progetto chiuso

Data della firma CE 8 Marzo 2023

Data di avvio 1 Maggio 2023

Data di completamento 30 Aprile 2026

Finanziato da

• The European Innovation Council (EIC)

Costo totale

€ 2 533 255,25

Contributo UE

€ 2 533 255,00

Coordinato da

UNIVERSIDAD DEL PAIS VASCO/ EUSKAL HERRIKO UNIBERTSITATEA
Spain

Periodic Reporting for period 1 - FUNAMBULIST (FUnctional Nucleic Acids as Versatile SMart BUilding BLocks in Non-ConventIonal SolvenTs)

Periodo di rendicontazione: 2023-05-01 al 2024-04-30

Sintesi del contesto e degli obiettivi generali del progetto

The main objective of FUNAMBULIST is to create a modular transversal toolkit allowed to control estereoselectivity for fine chemicals synthesis by leveraging the helical chirality of nucleic acids. This is achieved by replacing water with (chiral) ionic liquids or deep eutectic solvents as the bulk solvent. Despite initial hiring challenges, the project is now on track with strong collaboration and coordination among partners.

Lavoro eseguito dall’inizio del progetto fino alla fine del periodo coperto dalla relazione e principali risultati finora ottenuti

Objective 1: Achieve semi-preparative scale synthesis of unnatural XNA oligomers.
Successfully introduced biotin and primary amine modifications, key factor to facilitate the further immobilization -and its evaluation- on membranes.

Objective 2: Synthesize sequence-defined polymers and assessed their interaction with model ionic liquids. Initial studies of interactions between sequence-controlled polymers and ionic liquids, finding that their fundamental physico-chemical properties remain unaltered.

Objective 3: Develop a screening method for nucleic acid scaffolds and evaluated bi-molecular systems in ionic liquids. Implemented new microfluidic device designs and hydrogel beads for handling modified DNAs and XNAs.

Objective 4: Synthesized new compounds capable of enhance the catalysis monitoring in the Diels-Alder reaction. Initial studies regarding membrane modification by DNA-scaffolds

Objective 5: Created a Data Management Plan and developed the FUNAMBULIST website as a communication tool. Consortium meetings were carried out and future meetings are planned.

Objective 6: Designed a Quality Management Plan and established efficient document sharing through One-Drive folder created for FUNAMBULIST.
Held regular consortium meetings to monitor progress.

Progressi oltre lo stato dell’arte e potenziale impatto previsto (incluso l’impatto socioeconomico e le implicazioni sociali più ampie del progetto fino ad ora)

Development of a novel screening method for nucleic acid scaffolds with chemical base modifications, capable of catalyzing reactions.
Innovative microfluidic device designs compatible with the properties of ionic liquids.
Develop of a several sequence-defined polymers that can serve as platform for the membrane.
New fluorescent -and more reactive- compounds were created to control de monitoring of the catalysis.
Initial studies regarding DNA-immobilization over a membrane were carried out.

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