Graph Signal Processing Models for Neurovascular Decoupling.

Projektbeschreibung

Graphenmodelle für Hirngefäße zum Verständnis neurovaskulärer Prozesse

Der BOLD-Effekt (Blood Oxygenation Level Dependent) ist ein wichtiges fMRI-Verfahren zur Messung der Gehirnaktivität auf der Grundlage der neurovaskulären Kopplung. Faktoren wie physiologische Veränderungen und neurovaskulären Entkopplung erschweren jedoch die Interpretation der Signale. Da es keine Methode gibt, um neuronale Aktivität von vaskulären Reaktionen zu trennen, wird bei BOLD-Analysen die Rolle der Blutgefäße oft übersehen. Im Rahmen des über die Marie-Skłodowska-Curie-Maßnahmen unterstützten Projekts GRASPING NERVE wird angestrebt, ein Graphenmodell der Hirngefäße zu entwickeln, um die zerebrovaskuläre Konnektivität zu bewerten und BOLD-Signalschwankungen zu integrieren. Dieser nicht invasive Ansatz wird das Verständnis der neurovaskulären Kopplung verbessern und Studien über pathologische neurovaskuläre Entkopplung erleichtern. Dazu wird das Modell unter Verwendung hoher und ultrahoher MRT-Felder validiert, wobei BOLD- mit Nicht-BOLD-Verfahren verglichen werden.

Ziel

Blood Oxygenation Level Dependent (BOLD) signal is a widespread functional Magnetic Resonance Imaging (fMRI) technique to non-invasively study brain activity, and it relies on the mechanism of Neurovascular Coupling (NC), i.e. changes in cerebral blood flow driven by neuronal activity. However, there are various confounding factors for NC, such as homoeostatic physiological changes, or NC uncoupling driven by certain pathologies. Currently, there is no method to disentangle the information associated with neuronal activity from the vascular response. This is an important issue in both physiological imaging and brain activity investigation, as the two signal sources act as competing confounding factors. Furthermore, although vessels are the main blood distribution system, they are mostly ignored when taking into account BOLD analyses.
In this project, I will first create a graph model of cerebral vessels to assess “cerebrovascular connectivity”. I will then use graph signal processing, a novel signal processing technique based on graphs, to embed BOLD signal fluctuations into the vascular and the more traditional tractography-based graphs to disentangle the propagation of neuronal activity and blood flow in these two pathways. This technique will allow disentangling the components of NC non-invasively, offering new insight on brain activity and neurovascular coupling, and allowing further studies on pathological NC uncoupling, such as pre-surgical imaging for tumour.
I will apply this model at the macro- (whole-brain) and meso- (grey-matter- layers) scale, connecting the properties of NC in functional imaging between them. The application will be validated in high (3 Tesla) and ultra-high (7 Tesla) MRI fields to address its feasibility for both research and clinical application. Finally, BOLD will be compared with non-BOLD functional techniques, such as Arterial Spin Labelling and Vascular Space Occupancy, to shed light into the imaging of NC and brain activity.