Objective
From immunotherapy to restoring neural function in Parkinson’s disease, cell-based therapies are shaping medicine. Human pluripotent stem cells (hPSCs) could be an invaluable resource, given their unlimited in vitro self-renewal and ability to differentiate into any cell-type. However, in vitro hPSC-derived cells show defective performance in transplantation assays. Using the long-sought in vitro production of hematopoietic stem cells (HSCs) as a model, I propose a strategy to assess and rectify the accuracy of hPSC differentiation protocols in recapitulating embryonic cell fate decision processes:
AIM1 Establish an analysis workflow to systematically compare hPSC-derived developmental trajectories with in vivo mammalian embryonic development, using time-resolved single-cell RNA sequencing (scRNAseq) data. I will benchmark an hPSC differentiation dataset featuring emergence of an HSC signature against a range of published developmental datasets, including both human fetal samples and animal models.
AIM2 Probe intrinsic variability of embryoid body (EB)-based hPSC differentiation by integrating cell-type composition (by scRNAseq), time-lapse secretome and morphology of 24 individual EBs. I will thus identify cell-types and secreted factors enriched in the rare EBs comprising an HSC signature.
AIM3 Determine the functional relevance of genes identified as in vitro de-regulated (Aim 1) and/or associated with HSC signature emergence in vitro (Aim 2) using a targeted genetic screen in hPSC differentiation followed by robust transplantation in immunodeficient mice.
AIM4 Define the cell autonomous role of genes with confirmed functional relevance for production of HSCs from hPSC (Aim 3) by multiomic profiling of mosaic EBs.
By leveraging the rise in single-cell developmental Atlases, I will obtain robust generation of hPSC-derived HSCs through innovative declinations of EB-based hPSC differentiation, establishing a new paradigm towards clinical translation of hPSCs.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine immunology immunotherapy
- medical and health sciences clinical medicine transplantation
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2025-STG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
405 30 Goeteborg
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.