Cel Combating bacteria with antibiotics is an endless race because bacteria acquire antibiotic resistance (AR) genes easily from unknown environmental sources. Until now, understanding and control of AR spread was attempted through the precise descriptions of AR genes presently found in hospitals, and inference from this of the working mechanisms of dispersion (epidemiological approach). We think that an appropriate long-term public health objective would be to elucidate the molecular mechanisms behind the observed AR spread in a concerted strategy targeting the dissemination modules, from AR recruitment to their ultimate acquisition by bacterial pathogens. Thus, the focus of this project is to explore a mechanistic approach to combat AR. A coherent picture, which could explain the road to multiple AR in gram-negative species, is beginning to emerge from research on gene mobility. Starting from the superintegrons, which could be considered as the "cradles" of many AR, integron cassettes are recruited into integron platforms carried by transposons or plasmids. Insertion sequences (IS) capture genes or integron platforms to form transposons, which are subsequently translocated to conjugative plasmids. Plasmids transfer AR and establish themselves with variable efficiency in pathogenic bacteria. Finally, plasmids need to be stably inherited once they established themselves in a recipient cell. Specific mechanisms ensure that plasmid-free cells are eliminated. The objectives of CRAB deal with each of the dissemination modules in this chain ' integrons (WP1), transposons (WP2), conjugative plasmids (WP3) and stability modules (WP4) ' in a concerted approach. We concentrate on the as yet improperly studied aspects of these mechanisms. WP1: mechanism of integron cassette recombination, signals triggering integrase expression and molecular evolution of gene cassettes. WP2: Propensities of three major classes of IS to acquire, stabilise and vehicle AR genes and identification... Dziedzina nauki natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesgeneticsDNAmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibioticsmedical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistancenatural sciencesbiological sciencesmolecular biologymolecular evolution Program(-y) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Temat(-y) LSH-2004-2.1.2-2 - The role of mobile genetic elements in the generation of antimicrobial resistance LSH-2004-2.1.2-3 - Improved understanding of ecological factors with impact on the genetic and molecular determinants of fitness and virulence of resistant bacterial pathogens Zaproszenie do składania wniosków FP6-2004-LIFESCIHEALTH-5 Zobacz inne projekty w ramach tego zaproszenia System finansowania STREP - Specific Targeted Research Project Koordynator INSTITUT PASTEUR Wkład UE Brak danych Adres 25- 28, rue du Docteur Roux PARIS Francja Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych Uczestnicy (7) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Francja Wkład UE Brak danych Adres Rue Michel Ange, 3 PARIS Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych UNIVERSIDAD DE CANTABRIA Hiszpania Wkład UE Brak danych Adres Vicerrectorado de Investigación, Pabellón de Gobierno, Avda de los Castros s/n SANTANDER Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych AGRICULTURAL BIOTECHNOLOGY CENTER Węgry Wkład UE Brak danych Adres Szent-Györgyi Albert u. 4. pob 411 GÖDÖLLÖ Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych UNIVERSITAET GRAZ Austria Wkład UE Brak danych Adres Universitaetsplatz 3 GRAZ Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych UNIVERSITÉ LIBRE DE BRUXELLES Belgia Wkład UE Brak danych Adres 50 avenue F Roosevelt BRUXELLES Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych THE UNIVERSITY OF MANCHESTER Zjednoczone Królestwo Wkład UE Brak danych Adres Oxford Road MANCHESTER Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych DELPHI GENETICS SA Belgia Wkład UE Brak danych Adres Rue Adrienne Bolland 8 CHARLEROI Zobacz na mapie Linki Strona internetowa Opens in new window Koszt całkowity Brak danych
